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The Past, Present and Future of Flow Cytometry in Central Nervous System Malignancies

1
Faculty of Medicine, Neurosurgical Institute, School of Health Sciences, University of Ioannina, 45110 Ioannina, Greece
2
Haematology Laboratory-Unit of Molecular Biology, University Hospital of Ioannina, 45110 Ioannina, Greece
3
Department of Neurosurgery, University of Ioannina, 45110 Ioannina, Greece
*
Author to whom correspondence should be addressed.
Methods Protoc. 2021, 4(1), 11; https://doi.org/10.3390/mps4010011
Received: 9 January 2021 / Revised: 22 January 2021 / Accepted: 22 January 2021 / Published: 26 January 2021
Central nervous system malignancies (CNSMs) are categorized among the most aggressive and deadly types of cancer. The low median survival in patients with CNSMs is partly explained by the objective difficulties of brain surgeries as well as by the acquired chemoresistance of CNSM cells. Flow Cytometry is an analytical technique with the ability to quantify cell phenotype and to categorize cell populations on the basis of their characteristics. In the current review, we summarize the Flow Cytometry methodologies that have been used to study different phenotypic aspects of CNSMs. These include DNA content analysis for the determination of malignancy status and phenotypic characterization, as well as the methodologies used during the development of novel therapeutic agents. We conclude with the historical and current utility of Flow Cytometry in the field, and we propose how we can exploit current and possible future methodologies in the battle against this dreadful type of malignancy. View Full-Text
Keywords: central nervous system malignancies; flow cytometry; glioblastoma; intraoperative flow cytometry; phenotypic analysis; DNA content analysis central nervous system malignancies; flow cytometry; glioblastoma; intraoperative flow cytometry; phenotypic analysis; DNA content analysis
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MDPI and ACS Style

Vartholomatos, E.; Vartholomatos, G.; Alexiou, G.A.; Markopoulos, G.S. The Past, Present and Future of Flow Cytometry in Central Nervous System Malignancies. Methods Protoc. 2021, 4, 11. https://doi.org/10.3390/mps4010011

AMA Style

Vartholomatos E, Vartholomatos G, Alexiou GA, Markopoulos GS. The Past, Present and Future of Flow Cytometry in Central Nervous System Malignancies. Methods and Protocols. 2021; 4(1):11. https://doi.org/10.3390/mps4010011

Chicago/Turabian Style

Vartholomatos, Evrysthenis; Vartholomatos, George; Alexiou, George A.; Markopoulos, Georgios S. 2021. "The Past, Present and Future of Flow Cytometry in Central Nervous System Malignancies" Methods Protoc. 4, no. 1: 11. https://doi.org/10.3390/mps4010011

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