Next Article in Journal
Quantification of Ethanedinitrile in Air Using a New and Accurate Gas Chromatography Method
Previous Article in Journal
A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria
Article Menu
Issue 4 (December) cover image

Export Article

Open AccessArticle
Methods Protoc. 2018, 1(4), 47;

New Protocol for Cell Culture to Obtain Mitotic Chromosomes in Fishes

Department of Morphology, Sao Paulo State University, Botucatu 18618-689, Sao Paulo, Brazil
Department of Animal Reproduction and Radiology, Sao Paulo State University, UNESP, Botucatu 18618-681, Sao Paulo, Brazil
Author to whom correspondence should be addressed.
Received: 15 November 2018 / Revised: 5 December 2018 / Accepted: 7 December 2018 / Published: 10 December 2018
Full-Text   |   PDF [889 KB, uploaded 10 December 2018]   |  


Cell culture is an excellent alternative for the maintenance of cell lines and to obtain quality chromosome preparations of fishes. However, this methodology is still little employed, mainly because of the difficulty of standardization of cell cultures. In this study, we describe a methodology for the rapid acquisition of cell lineages and mitotic chromosomes for cytogenetic studies of fish species from muscle tissue cells. Our methodology is based on the use of a gelatin film, which provides better adhesion of a large number of cells and appropriate conditions for multiplication. The cells of Astyanax altiparanae, used as an experimental model, with fibroblast-like morphology, showed rapid cellular proliferation, resulting in a great number of cells. Chromosomal preparations of cultured cells showed the diploid number of the species, 2n = 50 chromosomes, in 80% of the cells examined, with chromosomes intact and distended. Cell populations were cryopreserved and after being recovered, these cells maintained their proliferative capacity. The development of this methodology represents an innovation for the fish cytogenetics area and it may bring a significant contribution to the conservation and study of several groups due to the difficulty of obtaining good-quality chromosome preparations. View Full-Text
Keywords: cell culture; cryopreservation; fish cytogenetic; chromosomes; mitosis cell culture; cryopreservation; fish cytogenetic; chromosomes; mitosis

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Paim, F.G.; Maia, L.; Landim-Alvarenga, F.C.; Foresti, F.; Oliveira, C. New Protocol for Cell Culture to Obtain Mitotic Chromosomes in Fishes. Methods Protoc. 2018, 1, 47.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Statistics



[Return to top]
Methods Protoc. EISSN 2409-9279 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top