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Methods and Protocols, Volume 1, Issue 4

December 2018 - 12 articles

Cover Story: In this study, we report the modification of the genetic transformation protocol for cassava, in order to obtain transgenic lines of a Brazilian recalcitrant cultivar, known as Verdinha BRS 222. View this paper
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Articles (12)

  • Article
  • Open Access
8 Citations
3,925 Views
9 Pages

New Protocol for Cell Culture to Obtain Mitotic Chromosomes in Fishes

  • Fabilene G. Paim,
  • Leandro Maia,
  • Fernanda da Cruz Landim-Alvarenga,
  • Fausto Foresti and
  • Claudio Oliveira

10 December 2018

Cell culture is an excellent alternative for the maintenance of cell lines and to obtain quality chromosome preparations of fishes. However, this methodology is still little employed, mainly because of the difficulty of standardization of cell cultur...

  • Protocol
  • Open Access
42 Citations
5,730 Views
15 Pages

A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria

  • Siripat Ngoennet,
  • Yasuhiro Nishikawa,
  • Takashi Hibino,
  • Rungaroon Waditee-Sirisattha and
  • Hakuto Kageyama

3 December 2018

This report provides a broadly applicable and cost-effective method for the purification of mycosporine-like amino acids (MAAs) from cyanobacteria. As MAAs are known to have multiple bioactivities for health and beauty, a universal isolation method o...

  • Benchmark
  • Open Access
9 Citations
3,824 Views
12 Pages

20 November 2018

We propose an optical tweezers setup based on an annular-shaped laser beam that is efficient to trap 2.8 μ m-diameter superparamagnetic particles. The optical trapping of such particles was fully characterized, and a direct absolute compariso...

  • Benchmark
  • Open Access
1 Citations
3,924 Views
11 Pages

Influence of Metabolite Extraction Methods on 1H-NMR-Based Metabolomic Profiling of Enteropathogenic Yersinia

  • Brandon R. Gines,
  • Willard E. Collier,
  • Mohamed A. Abdalla and
  • Teshome Yehualaeshet

20 November 2018

Metabolite extraction is one of the critical steps in microbial metabolome analysis. It affects both the observed metabolite content and biological interpretation of the data. Several methods exist for metabolite extraction of microbes, but the liter...

  • Protocol
  • Open Access
12 Citations
7,912 Views
8 Pages

19 November 2018

Segmentation is one of the most important steps in microscopy image analysis. Unfortunately, most of the methods use fluorescence images for this task, which is not suitable for analysis that requires a knowledge of area occupied by cells and an expe...

  • Technical Note
  • Open Access
11 Citations
4,310 Views
10 Pages

Genetic Transformation of Recalcitrant Cassava by Embryo Selection and Increased Hormone Levels

  • Ezequiel Matías Lentz,
  • Sabrina Eisner,
  • Emily Jane McCallum,
  • Kim Schlegel,
  • Francisco de Assis de Paiva Campos,
  • Wilhelm Gruissem and
  • Hervé Vanderschuren

13 November 2018

Genetic engineering is considered to be an important tool for the improvement of cassava. Cassava is a highly heterozygous crop species for which conventional breeding is a lengthy and tedious process. Robust transformation is based on Agrobacterium-...

  • Article
  • Open Access
2 Citations
3,246 Views
11 Pages

9 November 2018

A robust method was developed to investigate the liposomal behavior of novel enzymatically-synthesized hydroxytyrosol and tyrosol phospholipids. Bilayer characteristic obtained by this method, including bilayer formation stability and adsorption prop...

  • Protocol
  • Open Access
30 Citations
14,353 Views
16 Pages

Build Your Own Microscope: Step-By-Step Guide for Building a Prism-Based TIRF Microscope

  • Dalton R. Gibbs,
  • Anisa Kaur,
  • Anoja Megalathan,
  • Kumar Sapkota and
  • Soma Dhakal

3 November 2018

Prism-based total internal reflection fluorescence (pTIRF) microscopy is one of the most widely used techniques for the single molecule analysis of a vast range of samples including biomolecules, nanostructures, and cells, to name a few. It allows fo...

  • Review
  • Open Access
17 Citations
6,523 Views
20 Pages

Review of Biomedical Applications of Contactless Imaging of Neonates Using Infrared Thermography and Beyond

  • Abbas K. AlZubaidi,
  • Yahya Ethawi,
  • Georg M. Schmölzer,
  • Sherif Sherif,
  • Michael Narvey and
  • Molly Seshia

29 October 2018

The sick preterm infant monitoring is an intriguing job that medical staff in Neonatal Intensive Care Units (NICU) must deal with on a daily basis. As a standards monitoring procedure, preterm infants are monitored via sensors and electrodes that are...

  • Protocol
  • Open Access
5 Citations
6,124 Views
8 Pages

24 October 2018

Acceptance and commitment therapy (ACT) has been reported to be effective in the treatment of some psychiatric disorders. It remains uncertain, however, whether ACT is safe and effective in treating schizophrenia spectrum and other psychotic disorder...

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Methods Protoc. - ISSN 2409-9279Creative Common CC BY license