A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria
AbstractThis report provides a broadly applicable and cost-effective method for the purification of mycosporine-like amino acids (MAAs) from cyanobacteria. As MAAs are known to have multiple bioactivities for health and beauty, a universal isolation method of MAAs from biomass is attractive. In particular, the biomass of photosynthetic microorganisms such as cyanobacteria is of interest as a natural source of useful compound production, because of their photoautotrophic property. The method presented here is applicable for the isolation of mycosporine-2-glycine (M2G), which is a rare MAA produced in a halotolerant cyanobacterium. This method also allowed for the isolation of two of the most common MAAs, shinorine (SHI) and porphyra-334 (P334). A three-step separation process using low pressure liquid chromatography yielded purified MAAs, which were characterized by nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC/MS) analyses. The purified MAAs exhibited free radical scavenging activity in the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay. The experimental parameters obtained in this report may allow for a scale-up of the MAA purification process for future industrial applications. View Full-Text
Share & Cite This Article
Ngoennet, S.; Nishikawa, Y.; Hibino, T.; Waditee-Sirisattha, R.; Kageyama, H. A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria. Methods Protoc. 2018, 1, 46.
Ngoennet S, Nishikawa Y, Hibino T, Waditee-Sirisattha R, Kageyama H. A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria. Methods and Protocols. 2018; 1(4):46.Chicago/Turabian Style
Ngoennet, Siripat; Nishikawa, Yasuhiro; Hibino, Takashi; Waditee-Sirisattha, Rungaroon; Kageyama, Hakuto. 2018. "A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria." Methods Protoc. 1, no. 4: 46.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.