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Methods Protoc. 2018, 1(4), 46;

A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria

Department of Microbiology, Faculty of Science, Chulalongkorn University, Payathai Road, Pathumwan, Bangkok 10330, Thailand
Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tenpaku-ku, Nagoya, Aichi 468-8503, Japan
Faculty of Science and Technology, Meijo University, 1-501 Shiogamaguchi, Tenpaku-ku, Nagoya, Aichi 468-8502, Japan
Authors to whom correspondence should be addressed.
Received: 26 October 2018 / Revised: 27 November 2018 / Accepted: 30 November 2018 / Published: 3 December 2018
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This report provides a broadly applicable and cost-effective method for the purification of mycosporine-like amino acids (MAAs) from cyanobacteria. As MAAs are known to have multiple bioactivities for health and beauty, a universal isolation method of MAAs from biomass is attractive. In particular, the biomass of photosynthetic microorganisms such as cyanobacteria is of interest as a natural source of useful compound production, because of their photoautotrophic property. The method presented here is applicable for the isolation of mycosporine-2-glycine (M2G), which is a rare MAA produced in a halotolerant cyanobacterium. This method also allowed for the isolation of two of the most common MAAs, shinorine (SHI) and porphyra-334 (P334). A three-step separation process using low pressure liquid chromatography yielded purified MAAs, which were characterized by nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC/MS) analyses. The purified MAAs exhibited free radical scavenging activity in the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay. The experimental parameters obtained in this report may allow for a scale-up of the MAA purification process for future industrial applications. View Full-Text
Keywords: mycosporine-like amino acids; mycosporine-2-glycine; shinorine; porphyra-334 mycosporine-like amino acids; mycosporine-2-glycine; shinorine; porphyra-334

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Ngoennet, S.; Nishikawa, Y.; Hibino, T.; Waditee-Sirisattha, R.; Kageyama, H. A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria. Methods Protoc. 2018, 1, 46.

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