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Methods Protoc. 2018, 1(4), 46; https://doi.org/10.3390/mps1040046

A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria

1
Department of Microbiology, Faculty of Science, Chulalongkorn University, Payathai Road, Pathumwan, Bangkok 10330, Thailand
2
Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tenpaku-ku, Nagoya, Aichi 468-8503, Japan
3
Faculty of Science and Technology, Meijo University, 1-501 Shiogamaguchi, Tenpaku-ku, Nagoya, Aichi 468-8502, Japan
*
Authors to whom correspondence should be addressed.
Received: 26 October 2018 / Revised: 27 November 2018 / Accepted: 30 November 2018 / Published: 3 December 2018
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Abstract

This report provides a broadly applicable and cost-effective method for the purification of mycosporine-like amino acids (MAAs) from cyanobacteria. As MAAs are known to have multiple bioactivities for health and beauty, a universal isolation method of MAAs from biomass is attractive. In particular, the biomass of photosynthetic microorganisms such as cyanobacteria is of interest as a natural source of useful compound production, because of their photoautotrophic property. The method presented here is applicable for the isolation of mycosporine-2-glycine (M2G), which is a rare MAA produced in a halotolerant cyanobacterium. This method also allowed for the isolation of two of the most common MAAs, shinorine (SHI) and porphyra-334 (P334). A three-step separation process using low pressure liquid chromatography yielded purified MAAs, which were characterized by nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC/MS) analyses. The purified MAAs exhibited free radical scavenging activity in the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay. The experimental parameters obtained in this report may allow for a scale-up of the MAA purification process for future industrial applications. View Full-Text
Keywords: mycosporine-like amino acids; mycosporine-2-glycine; shinorine; porphyra-334 mycosporine-like amino acids; mycosporine-2-glycine; shinorine; porphyra-334
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Ngoennet, S.; Nishikawa, Y.; Hibino, T.; Waditee-Sirisattha, R.; Kageyama, H. A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria. Methods Protoc. 2018, 1, 46.

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