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Open AccessTechnical Note
Methods Protoc. 2018, 1(4), 42;

Genetic Transformation of Recalcitrant Cassava by Embryo Selection and Increased Hormone Levels

Department of Biology, Plant Biotechnology, ETH Zurich, 8092 Zürich, Switzerland
Department of Biochemistry and Molecular Biology, Universidade Federal do Ceará, 60455-900 Fortaleza, Brazil
Plant Genetics, TERRA Teaching and Research Center, University of Liège, Passage des Déportés 2, 5030 Gembloux, Belgium
Author to whom correspondence should be addressed.
Received: 28 August 2018 / Revised: 30 October 2018 / Accepted: 30 October 2018 / Published: 13 November 2018
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Genetic engineering is considered to be an important tool for the improvement of cassava. Cassava is a highly heterozygous crop species for which conventional breeding is a lengthy and tedious process. Robust transformation is based on Agrobacterium-mediated transformation of friable embryogenic callus (FEC). Production of FEC is genotype-dependent and considered to be a major bottleneck for the genetic transformation of cassava. As a consequence, routine genetic transformation has only been established for a handful of cassava cultivars. Therefore, development of procedures enabling efficient production of high-quality cassava FEC is required to allow the translation of research from the model cultivar to farmer-preferred cassava cultivars. Here we study the FEC production capacity of Brazilian cassava cultivars and report the modification of the protocol for the genetic transformation of Verdinha (BRS 222), a recalcitrant cultivar with high potential for protein production that is extensively used by farmers in Brazil. View Full-Text
Keywords: cassava; somatic embryo; FEC; transformation; Agrobacterium; farmer-preferred cultivar cassava; somatic embryo; FEC; transformation; Agrobacterium; farmer-preferred cultivar

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Lentz, E.M.; Eisner, S.; McCallum, E.J.; Schlegel, K.; Campos, F.A.P.; Gruissem, W.; Vanderschuren, H. Genetic Transformation of Recalcitrant Cassava by Embryo Selection and Increased Hormone Levels. Methods Protoc. 2018, 1, 42.

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