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Methods Protoc. 2018, 1(4), 42; https://doi.org/10.3390/mps1040042

Genetic Transformation of Recalcitrant Cassava by Embryo Selection and Increased Hormone Levels

1
Department of Biology, Plant Biotechnology, ETH Zurich, 8092 Zürich, Switzerland
2
Department of Biochemistry and Molecular Biology, Universidade Federal do Ceará, 60455-900 Fortaleza, Brazil
3
Plant Genetics, TERRA Teaching and Research Center, University of Liège, Passage des Déportés 2, 5030 Gembloux, Belgium
*
Author to whom correspondence should be addressed.
Received: 28 August 2018 / Revised: 30 October 2018 / Accepted: 30 October 2018 / Published: 13 November 2018
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Abstract

Genetic engineering is considered to be an important tool for the improvement of cassava. Cassava is a highly heterozygous crop species for which conventional breeding is a lengthy and tedious process. Robust transformation is based on Agrobacterium-mediated transformation of friable embryogenic callus (FEC). Production of FEC is genotype-dependent and considered to be a major bottleneck for the genetic transformation of cassava. As a consequence, routine genetic transformation has only been established for a handful of cassava cultivars. Therefore, development of procedures enabling efficient production of high-quality cassava FEC is required to allow the translation of research from the model cultivar to farmer-preferred cassava cultivars. Here we study the FEC production capacity of Brazilian cassava cultivars and report the modification of the protocol for the genetic transformation of Verdinha (BRS 222), a recalcitrant cultivar with high potential for protein production that is extensively used by farmers in Brazil. View Full-Text
Keywords: cassava; somatic embryo; FEC; transformation; Agrobacterium; farmer-preferred cultivar cassava; somatic embryo; FEC; transformation; Agrobacterium; farmer-preferred cultivar
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Lentz, E.M.; Eisner, S.; McCallum, E.J.; Schlegel, K.; Campos, F.A.P.; Gruissem, W.; Vanderschuren, H. Genetic Transformation of Recalcitrant Cassava by Embryo Selection and Increased Hormone Levels. Methods Protoc. 2018, 1, 42.

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