Genetic Transformation of Recalcitrant Cassava by Embryo Selection and Increased Hormone Levels
AbstractGenetic engineering is considered to be an important tool for the improvement of cassava. Cassava is a highly heterozygous crop species for which conventional breeding is a lengthy and tedious process. Robust transformation is based on Agrobacterium-mediated transformation of friable embryogenic callus (FEC). Production of FEC is genotype-dependent and considered to be a major bottleneck for the genetic transformation of cassava. As a consequence, routine genetic transformation has only been established for a handful of cassava cultivars. Therefore, development of procedures enabling efficient production of high-quality cassava FEC is required to allow the translation of research from the model cultivar to farmer-preferred cassava cultivars. Here we study the FEC production capacity of Brazilian cassava cultivars and report the modification of the protocol for the genetic transformation of Verdinha (BRS 222), a recalcitrant cultivar with high potential for protein production that is extensively used by farmers in Brazil. View Full-Text
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Lentz, E.M.; Eisner, S.; McCallum, E.J.; Schlegel, K.; Campos, F.A.P.; Gruissem, W.; Vanderschuren, H. Genetic Transformation of Recalcitrant Cassava by Embryo Selection and Increased Hormone Levels. Methods Protoc. 2018, 1, 42.
Lentz EM, Eisner S, McCallum EJ, Schlegel K, Campos FAP, Gruissem W, Vanderschuren H. Genetic Transformation of Recalcitrant Cassava by Embryo Selection and Increased Hormone Levels. Methods and Protocols. 2018; 1(4):42.Chicago/Turabian Style
Lentz, Ezequiel M.; Eisner, Sabrina; McCallum, Emily J.; Schlegel, Kim; Campos, Francisco A.P.; Gruissem, Wilhelm; Vanderschuren, Hervé. 2018. "Genetic Transformation of Recalcitrant Cassava by Embryo Selection and Increased Hormone Levels." Methods Protoc. 1, no. 4: 42.
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