Special Issue "Single-Molecule Techniques"
A special issue of Methods and Protocols (ISSN 2409-9279).
Deadline for manuscript submissions: closed (31 October 2018)
The recent implementation of state-of-the-art single-molecule techniques in cross-disciplinary science has transformed our understanding of biology and physics of life. Single-molecule techniques are the most desirable tool kits in many aspects of research today, not only to answer fundamental questions but also to enable high-sensitivity detection of small molecules and biomarkers. With their ever-improving spatial and temporal resolution, single-molecule techniques such as fluorescence microscopy, optical tweezers, and atomic force microscopy (AFM) have enabled single-molecule analysis of increasingly complex systems, such as live cells. Not amenable by bulk biochemical methods—because of averaging over large populations of molecules—single-molecule studies are significant to map reaction trajectories of individual molecules without the need for synchronization and to identify properties displayed by various molecular subpopulations. In addition, contrary to traditional ensemble methods that rely mainly in or close to the state of equilibrium, single-molecule techniques such as optical tweezers and AFM can illuminate the mechanism of biomolecular mechanics under strained conditions.
In this Special Issue on “Single-Molecule Techniques”, we welcome both original research and review articles as well as protocols pertaining to current applications of single-molecule techniques in many different areas of scientific inquiry including structural studies, DNA- as well as protein-DNA biophysics, enzymology, chemical biology, medicinal chemistry, nanotechnology and many more. In addition, we aim to present exemplary research findings from single-molecule studies.
Dr. Soma Dhakal
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Methods and Protocols is an international peer-reviewed open access quarterly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) is waived for well-prepared manuscripts submitted to this issue. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.
- Fluorescence Microscopy
- Fluorescence resonance energy transfer (FRET)
- Optical Tweezers
- Atomic Force Microscopy (AFM)
- Mechanical manipulation