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10.3390/mps1040037
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Open AccessTechnical Note

DNA Extraction with DNAzol and LAMP, Performed in a Heating Block as a Simple Procedure for Detection of Mycobacterium tuberculosis in Sputum Specimens

by
Álvaro Rodríguez-García
1,2,
Rosa E. Mares
1,
Patricia L. A. Muñoz
1,
Samuel G. Meléndez-López
1,
Alexei F. Licea-Navarro
3 and
Marco A. Ramos
1,*
1
Facultad de Ciencias Químicas e Ingeniería, Universidad Autónoma de Baja California, Calzada Universidad 14418, Tijuana, BCN 22390, Mexico
2
Laboratorio de Diagnóstico Clínico, Hospital General de Tijuana, Vía de la Juventud Oriente 4910, Tijuana, BCN 22010, Mexico
3
Departamento de Innovación Biomédica, Centro de Investigación Científica y Educación Superior de Ensenada, Carretera Ensenada-Tijuana 3918, Ensenada, BCN 22860, Mexico
*
Author to whom correspondence should be addressed.
Methods Protoc. 2018, 1(4), 37; https://doi.org/10.3390/mps1040037
Received: 25 September 2018 / Revised: 16 October 2018 / Accepted: 19 October 2018 / Published: 23 October 2018
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Abstract

Tuberculosis (TB) remains as a major public health issue in developing countries. Accurate detection is essential for the proper management of patients with active disease. Here, we present a simple DNAzol-LAMP (loop-mediated isothermal amplification) procedure for the detection of Mycobacterium tuberculosis in sputum specimens. Twenty smear-positive sputum samples were analyzed as follows: (i) Genetic material was extracted by a standard DNAzol protocol, and (ii) mycobacterial DNA was detected by a typical TB-specific loop-mediated isothermal amplification method. Results and diagnostic test performance attests to the suitability of the proposed procedure. View Full-Text
Keywords: tuberculosis; molecular detection; loop-mediated isothermal amplification tuberculosis; molecular detection; loop-mediated isothermal amplification
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
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MDPI and ACS Style

Rodríguez-García, Á.; Mares, R.E.; Muñoz, P.L.A.; Meléndez-López, S.G.; Licea-Navarro, A.F.; Ramos, M.A. DNA Extraction with DNAzol and LAMP, Performed in a Heating Block as a Simple Procedure for Detection of Mycobacterium tuberculosis in Sputum Specimens. Methods Protoc. 2018, 1, 37. https://doi.org/10.3390/mps1040037

AMA Style

Rodríguez-García Á, Mares RE, Muñoz PLA, Meléndez-López SG, Licea-Navarro AF, Ramos MA. DNA Extraction with DNAzol and LAMP, Performed in a Heating Block as a Simple Procedure for Detection of Mycobacterium tuberculosis in Sputum Specimens. Methods and Protocols. 2018; 1(4):37. https://doi.org/10.3390/mps1040037

Chicago/Turabian Style

Rodríguez-García, Álvaro; Mares, Rosa E.; Muñoz, Patricia L.A.; Meléndez-López, Samuel G.; Licea-Navarro, Alexei F.; Ramos, Marco A. 2018. "DNA Extraction with DNAzol and LAMP, Performed in a Heating Block as a Simple Procedure for Detection of Mycobacterium tuberculosis in Sputum Specimens" Methods Protoc. 1, no. 4: 37. https://doi.org/10.3390/mps1040037

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