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Methods Protoc. 2018, 1(4), 37; https://doi.org/10.3390/mps1040037

DNA Extraction with DNAzol and LAMP, Performed in a Heating Block as a Simple Procedure for Detection of Mycobacterium tuberculosis in Sputum Specimens

1
Facultad de Ciencias Químicas e Ingeniería, Universidad Autónoma de Baja California, Calzada Universidad 14418, Tijuana, BCN 22390, Mexico
2
Laboratorio de Diagnóstico Clínico, Hospital General de Tijuana, Vía de la Juventud Oriente 4910, Tijuana, BCN 22010, Mexico
3
Departamento de Innovación Biomédica, Centro de Investigación Científica y Educación Superior de Ensenada, Carretera Ensenada-Tijuana 3918, Ensenada, BCN 22860, Mexico
*
Author to whom correspondence should be addressed.
Received: 25 September 2018 / Revised: 16 October 2018 / Accepted: 19 October 2018 / Published: 23 October 2018
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Abstract

Tuberculosis (TB) remains as a major public health issue in developing countries. Accurate detection is essential for the proper management of patients with active disease. Here, we present a simple DNAzol-LAMP (loop-mediated isothermal amplification) procedure for the detection of Mycobacterium tuberculosis in sputum specimens. Twenty smear-positive sputum samples were analyzed as follows: (i) Genetic material was extracted by a standard DNAzol protocol, and (ii) mycobacterial DNA was detected by a typical TB-specific loop-mediated isothermal amplification method. Results and diagnostic test performance attests to the suitability of the proposed procedure. View Full-Text
Keywords: tuberculosis; molecular detection; loop-mediated isothermal amplification tuberculosis; molecular detection; loop-mediated isothermal amplification
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Rodríguez-García, Á.; Mares, R.E.; Muñoz, P.L.A.; Meléndez-López, S.G.; Licea-Navarro, A.F.; Ramos, M.A. DNA Extraction with DNAzol and LAMP, Performed in a Heating Block as a Simple Procedure for Detection of Mycobacterium tuberculosis in Sputum Specimens. Methods Protoc. 2018, 1, 37.

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