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Methods Protoc. 2018, 1(2), 12; https://doi.org/10.3390/mps1020012

Paramyxovirus Infections in Ex Vivo Lung Slice Cultures of Different Host Species

1
Department of Viroscience, Erasmus MC, P.O. Box 2040, 3000 CA Rotterdam, The Netherlands
2
Department of Microbiology, Boston University School of Medicine, Boston, MA 02118, USA
*
Author to whom correspondence should be addressed.
Received: 20 February 2018 / Revised: 11 March 2018 / Accepted: 21 March 2018 / Published: 27 March 2018
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Abstract

In vivo experiments in animal models of disease are of crucial importance for viral tropism and pathogenesis studies. However, these experiments must be complemented with in vitro and ex vivo experiments. Here, we describe a protocol for the preparation and ex vivo infection of lung slices from different mammalian host species with various respiratory paramyxoviruses expressing fluorescent reporter proteins, and suggest follow-up experiments including immunohistochemistry, flow cytometry and confocal microscopy. View Full-Text
Keywords: lung slice; paramyxovirus; respiratory virus; ex vivo model; infection; pathogenesis lung slice; paramyxovirus; respiratory virus; ex vivo model; infection; pathogenesis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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de Vries, R.D.; Rennick, L.J.; Duprex, W.P.; de Swart, R.L. Paramyxovirus Infections in Ex Vivo Lung Slice Cultures of Different Host Species. Methods Protoc. 2018, 1, 12.

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