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Insights into the Global Transcriptome Response of Lentinula edodes Mycelia during Aging
 
 
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Article

HOG1 Mitogen-Activated Protein Kinase Pathway–Related Autophagy Induced by H2O2 in Lentinula edodes Mycelia

by
Dong Yan
1,
Yangyang Fan
1,
Shuang Song
1,
Yuan Guo
1,
Yu Liu
1,
Xiaoling Xu
2,
Fang Liu
2,
Qi Gao
1,* and
Shouxian Wang
1,*
1
Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, 9 Shuguang Garden Zhonglu, Haidian District, Beijing 100097, China
2
College of Agriculture and Food Engineering, Baise University, 21 Zhongshan Second Street, Youjiang District, Baise 533000, China
*
Authors to whom correspondence should be addressed.
J. Fungi 2023, 9(4), 413; https://doi.org/10.3390/jof9040413
Submission received: 28 January 2023 / Revised: 8 March 2023 / Accepted: 24 March 2023 / Published: 28 March 2023
(This article belongs to the Special Issue Genomics and Evolution of Macrofungi)
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Abstract

Mycelial ageing is associated with ROS and autophagy in Lentinula edodes. However, the underlying cellular and molecular mechanisms between ROS and autophagy remain obscure. This study induced autophagy in L. edodes mycelia through exogenous H2O2 treatment. Results showed that 100 μM H2O2 treatment for 24 h significantly inhibited mycelial growth. H2O2 caused the depolarisation of MMP and accumulation of TUNEL-positive nuclei, which was similar to the ageing phenotype of L. edodes mycelia. Transcriptome analysis showed that differentially expressed genes were enriched in the mitophagic, autophagic, and MAPK pathways. LeAtg8 and LeHog1 were selected as hub genes. RNA and protein levels of LeATG8 increased in the H2O2-treated mycelia. Using fluorescent labelling, we observed for the first time the classic ring structure of autophagosomes in a mushroom, while 3D imaging suggested that these autophagosomes surrounded the nuclei to degrade them at specific growth stages. Phospho-LeHOG1 protein can translocate from the cytoplasm to the nucleus to regulate mycelial cells, resisting ROS-induced oxidative stress. Furthermore, LeATG8 expression was suppressed when LeHOG1 phosphorylation was inhibited. These results suggest that the LeATG8-dependent autophagy in L. edodes mycelial is closely associated with the activity or even phosphorylation of LeHOG1.
Keywords: Lentinula edodes; H2O2; autophagy; LeATG8; LeHOG1 Lentinula edodes; H2O2; autophagy; LeATG8; LeHOG1

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MDPI and ACS Style

Yan, D.; Fan, Y.; Song, S.; Guo, Y.; Liu, Y.; Xu, X.; Liu, F.; Gao, Q.; Wang, S. HOG1 Mitogen-Activated Protein Kinase Pathway–Related Autophagy Induced by H2O2 in Lentinula edodes Mycelia. J. Fungi 2023, 9, 413. https://doi.org/10.3390/jof9040413

AMA Style

Yan D, Fan Y, Song S, Guo Y, Liu Y, Xu X, Liu F, Gao Q, Wang S. HOG1 Mitogen-Activated Protein Kinase Pathway–Related Autophagy Induced by H2O2 in Lentinula edodes Mycelia. Journal of Fungi. 2023; 9(4):413. https://doi.org/10.3390/jof9040413

Chicago/Turabian Style

Yan, Dong, Yangyang Fan, Shuang Song, Yuan Guo, Yu Liu, Xiaoling Xu, Fang Liu, Qi Gao, and Shouxian Wang. 2023. "HOG1 Mitogen-Activated Protein Kinase Pathway–Related Autophagy Induced by H2O2 in Lentinula edodes Mycelia" Journal of Fungi 9, no. 4: 413. https://doi.org/10.3390/jof9040413

APA Style

Yan, D., Fan, Y., Song, S., Guo, Y., Liu, Y., Xu, X., Liu, F., Gao, Q., & Wang, S. (2023). HOG1 Mitogen-Activated Protein Kinase Pathway–Related Autophagy Induced by H2O2 in Lentinula edodes Mycelia. Journal of Fungi, 9(4), 413. https://doi.org/10.3390/jof9040413

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