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Open AccessArticle

Novel Blotting Method for Mass Spectrometry Imaging of Metabolites in Strawberry Fruit by Desorption/Ionization Using Through Hole Alumina Membrane

1
Department of Biosciences, Faculty of Science and Engineering, Teikyo University, Utsunomiya 320-8551, Japan
2
Division of Integrated Science and Engineering, Graduate School of Science and Engineering, Teikyo University, Utsunomiya 320-8551, Japan
3
Advanced Instrumental Analysis Center, Teikyo University, Utsunomiya 320-8551, Japan
4
Hamamatsu Photonics K.K., 314-5 Shimokanzo, Iwata 438-0193, Japan
*
Author to whom correspondence should be addressed.
Foods 2020, 9(4), 408; https://doi.org/10.3390/foods9040408
Received: 28 February 2020 / Revised: 26 March 2020 / Accepted: 27 March 2020 / Published: 1 April 2020
(This article belongs to the Special Issue Advancement of Mass Spectrometry Imaging for Food Science)
Mass spectrometry imaging (MSI) using matrix-assisted laser desorption/ionization (MALDI) is a powerful technique for visualizing metabolites in the strawberry fruit. During sample preparation for MALDI-MSI, sectioning of the samples is usually required. In general, MALDI-MSI analysis of strawberry fruits that are larger than a single glass slide is difficult because thin sections cannot be prepared. In this study, we attempted to visualize metabolites in large strawberry fruits by MSI, employing a blotting method that uses desorption ionization using a through-hole alumina membrane (DIUTHAME) chip. Large strawberry fruits were cut and a DIUTHAME chip was set on the cross-section to blot the metabolites. After drying the DIUTHAME chip, the metabolites were measured in positive and negative ion modes using a commercial MALDI-type mass spectrometer. Several peaks were detected in both the ion modes. Various metabolites related to food quality, such as sugars, organic acids, and anthocyanins, were detected and successfully visualized by blotting on a DIUTHAME chip in MSI. These results suggest that blotting using a DIUTHAME chip in MSI is useful for visualizing the metabolites present in the strawberry fruit. View Full-Text
Keywords: strawberry; metabolites; mass spectrometry imaging; desorption ionization using through hole alumina membrane (DIUTHAME); blotting strawberry; metabolites; mass spectrometry imaging; desorption ionization using through hole alumina membrane (DIUTHAME); blotting
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Enomoto, H.; Kotani, M.; Ohmura, T. Novel Blotting Method for Mass Spectrometry Imaging of Metabolites in Strawberry Fruit by Desorption/Ionization Using Through Hole Alumina Membrane. Foods 2020, 9, 408.

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