Behavior of Salmonella and Listeria monocytogenes in Raw Yellowfin Tuna during Cold Storage
Abstract
:1. Introduction
2. Materials and Methods
2.1. Target Pathogen Strains and Culture Preparation
2.2. Tuna Samples and Inoculation of Target Pathogens
2.3. Determination of Aerobic Plate Counts (APC) and Psychrotrophic Bacterial Counts (PBC)
2.4. Enumeration of Target Pathogens in Inoculated Tuna
2.5. Data Analysis
3. Results and Discussion
3.1. Changes of Aerobic Plate Counts (APC) and Psychrotrophic Bacterial Counts (PBC) in Tuna during Refrigerated and Frozen Storage
3.2. Behavior of Salmonella and Listeria monocytogenes in Tuna during Refrigeration Storage
3.3. Survival of Salmonella and L. monocytogenes in Tuna during Frozen Storage
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Pathogen | Gene | Primer | Sequence (5′–3′) | Size (bp) |
---|---|---|---|---|
Salmonella | invA | invAF | GTGAAATTATCGCCACGTTCGGGCAA | 284 |
invAR | TCATCGCACCGTCAAAGGAACC | |||
L. monocytogenes | lmo2234 | lmoF | TGTCCAGTTCCATTTTTAACT | 420 |
lmoR | TTGTTGTTCTGCTGTACGA |
Bacterial Counts | Day 0 | Day 2 | Day 4 | Day 6 | Day 8 | Day 10 | Day 12 |
---|---|---|---|---|---|---|---|
APC | 3.69 ± 0.06 a | 3.80 ± 0.09 a | 4.95 ± 0.05 b | 7.36 ± 0.02 c | 8.03 ± 0.08 d | 8.53 ± 0.17 e | 8.75 ± 0.08 e |
PBC | 3.98 ± 0.02 a | 3.98 ± 0.06 a | 5.57 ± 0.14 b | 7.61 ± 0.01 c | 8.73 ± 0.05 d | 9.11 ± 0.06 e | 9.18 ± 0.01 e |
Bacterial Counts | Day 0 | Day 14 | Day 28 | Day 42 | Day 56 | Day 70 | Day 84 |
---|---|---|---|---|---|---|---|
APC | 4.89 ± 0.06 a | 5.00 ± 0.03 a | 4.91 ± 0.05 a | 4.96 ± 0.05 a | 5.07 ± 0.03 a | 5.08 ± 0.04 a | 5.09 ± 0.01 a |
PBC | 5.30 ± 0.02 a | 4.93 ± 0.18 a | 5.01 ± 0.03 a | 5.13 ± 0.02 a | 5.13 ± 0.01 a | 5.17 ± 0.02 a | 5.12 ± 0.01 a |
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Liu, C.; Mou, J.; Su, Y.-C. Behavior of Salmonella and Listeria monocytogenes in Raw Yellowfin Tuna during Cold Storage. Foods 2016, 5, 16. https://doi.org/10.3390/foods5010016
Liu C, Mou J, Su Y-C. Behavior of Salmonella and Listeria monocytogenes in Raw Yellowfin Tuna during Cold Storage. Foods. 2016; 5(1):16. https://doi.org/10.3390/foods5010016
Chicago/Turabian StyleLiu, Chengchu, Jing Mou, and Yi-Cheng Su. 2016. "Behavior of Salmonella and Listeria monocytogenes in Raw Yellowfin Tuna during Cold Storage" Foods 5, no. 1: 16. https://doi.org/10.3390/foods5010016
APA StyleLiu, C., Mou, J., & Su, Y.-C. (2016). Behavior of Salmonella and Listeria monocytogenes in Raw Yellowfin Tuna during Cold Storage. Foods, 5(1), 16. https://doi.org/10.3390/foods5010016