A Rapid RPA-CRISPR/Cas12a Detection Method for Adulteration of Goat Milk Powder
Abstract
1. Introduction
2. Materials and Methods
2.1. Materials
2.2. DNA Extraction
2.3. Primer Design and RPA Amplification
2.4. Establishment of RPA-CRISPR/Cas12a Assay
2.5. Specificity and Detectability of RPA-CRISPR/Cas12a
2.6. Real Sample Testing and Statistical Analysis
2.7. Method Validation
3. Results and Discussion
3.1. Primers Screening and RPA Optimization
3.2. Establishment of RPA-CRISPR/Cas12a Method
3.3. The Specificity and Sensitivity of RPA-CRISPR/Cas12a Method
3.4. Real Sample Testing
3.5. Method Validation
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Huang, S.; Liu, Y.; Zhang, X.; Gai, Z.; Lei, H.; Shen, X. A Rapid RPA-CRISPR/Cas12a Detection Method for Adulteration of Goat Milk Powder. Foods 2023, 12, 1569. https://doi.org/10.3390/foods12081569
Huang S, Liu Y, Zhang X, Gai Z, Lei H, Shen X. A Rapid RPA-CRISPR/Cas12a Detection Method for Adulteration of Goat Milk Powder. Foods. 2023; 12(8):1569. https://doi.org/10.3390/foods12081569
Chicago/Turabian StyleHuang, Shuqin, Yan Liu, Xu Zhang, Zuoqi Gai, Hongtao Lei, and Xing Shen. 2023. "A Rapid RPA-CRISPR/Cas12a Detection Method for Adulteration of Goat Milk Powder" Foods 12, no. 8: 1569. https://doi.org/10.3390/foods12081569
APA StyleHuang, S., Liu, Y., Zhang, X., Gai, Z., Lei, H., & Shen, X. (2023). A Rapid RPA-CRISPR/Cas12a Detection Method for Adulteration of Goat Milk Powder. Foods, 12(8), 1569. https://doi.org/10.3390/foods12081569