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Article

Perilla frutescens Leaf Extract and Fractions: Polyphenol Composition, Antioxidant, Enzymes (α-Glucosidase, Acetylcholinesterase, and Tyrosinase) Inhibitory, Anticancer, and Antidiabetic Activities

by 1,2, 1,3,4,5,*, 5, 3, 6 and 1,3,4
1
College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, China
2
College of Life Sciences, Southwest Forestry University, Kunming 650224, China
3
College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China
4
National R&D Center for Freshwater Fish Processing, Jiangxi Normal University, Nanchang 330022, China
5
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
6
Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia
*
Author to whom correspondence should be addressed.
Academic Editor: Kwang-Geun Lee
Foods 2021, 10(2), 315; https://doi.org/10.3390/foods10020315
Received: 16 January 2021 / Revised: 29 January 2021 / Accepted: 30 January 2021 / Published: 3 February 2021
This study aims to evaluate the bioactive components, in vitro bioactivities, and in vivo hypoglycemic effect of P. frutescens leaf, which is a traditional medicine-food homology plant. P. frutescens methanol crude extract and its fractions (petroleum ether, chloroform, ethyl acetate, n-butanol fractions, and aqueous phase residue) were prepared by ultrasound-enzyme assisted extraction and liquid–liquid extraction. Among the samples, the ethyl acetate fraction possessed the high total phenolic (440.48 μg GAE/mg DE) and flavonoid content (455.22 μg RE/mg DE), the best antioxidant activity (the DPPH radical, ABTS radical, and superoxide anion scavenging activity, and ferric reducing antioxidant power were 1.71, 1.14, 2.40, 1.29, and 2.4 times higher than that of control Vc, respectively), the most powerful α-glucosidase inhibitory ability with the IC50 value of 190.03 μg/mL which was 2.2-folds higher than control acarbose, the strongest proliferative inhibitory ability against MCF-7 and HepG2 cell with the IC50 values of 37.92 and 13.43 μg/mL, which were considerable with control cisplatin, as well as certain inhibition abilities on acetylcholinesterase and tyrosinase. HPLC analysis showed that the luteolin, rosmarinic acid, rutin, and catechin were the dominant components of the ethyl acetate fraction. Animal experiments further demonstrated that the ethyl acetate fraction could significantly decrease the serum glucose level, food, and water intake of streptozotocin-induced diabetic SD rats, increase the body weight, modulate their serum levels of TC, TG, HDL-C, and LDL-C, improve the histopathology and glycogen accumulation in liver and intestinal tissue. Taken together, P. frutescens leaf exhibits excellent hypoglycemic activity in vitro and in vivo, and could be exploited as a source of natural antidiabetic agent. View Full-Text
Keywords: P. frutescens leaf; phytochemical composition; antioxidant activity; enzyme inhibitory; anticancer ability; antidiabetic activity P. frutescens leaf; phytochemical composition; antioxidant activity; enzyme inhibitory; anticancer ability; antidiabetic activity
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MDPI and ACS Style

Wang, Z.; Tu, Z.; Xie, X.; Cui, H.; Kong, K.W.; Zhang, L. Perilla frutescens Leaf Extract and Fractions: Polyphenol Composition, Antioxidant, Enzymes (α-Glucosidase, Acetylcholinesterase, and Tyrosinase) Inhibitory, Anticancer, and Antidiabetic Activities. Foods 2021, 10, 315. https://doi.org/10.3390/foods10020315

AMA Style

Wang Z, Tu Z, Xie X, Cui H, Kong KW, Zhang L. Perilla frutescens Leaf Extract and Fractions: Polyphenol Composition, Antioxidant, Enzymes (α-Glucosidase, Acetylcholinesterase, and Tyrosinase) Inhibitory, Anticancer, and Antidiabetic Activities. Foods. 2021; 10(2):315. https://doi.org/10.3390/foods10020315

Chicago/Turabian Style

Wang, Zhenxing, Zongcai Tu, Xing Xie, Hao Cui, Kin W. Kong, and Lu Zhang. 2021. "Perilla frutescens Leaf Extract and Fractions: Polyphenol Composition, Antioxidant, Enzymes (α-Glucosidase, Acetylcholinesterase, and Tyrosinase) Inhibitory, Anticancer, and Antidiabetic Activities" Foods 10, no. 2: 315. https://doi.org/10.3390/foods10020315

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