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Chemosensors 2018, 6(2), 18; https://doi.org/10.3390/chemosensors6020018

The Use of Hoechst Dyes for DNA Staining and Beyond

1
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany
2
Department of Cellular Logistics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany
*
Authors to whom correspondence should be addressed.
Received: 20 March 2018 / Revised: 15 April 2018 / Accepted: 17 April 2018 / Published: 18 April 2018
(This article belongs to the Special Issue Fluorescent Probes for Live Cell Imaging)
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Abstract

Hoechst dyes are among the most popular fluorophores used to stain DNA in living and fixed cells. Moreover, their high affinity and specificity towards DNA make Hoechst dyes excellent targeting moieties, which can be conjugated to various other molecules in order to tether them to DNA. The recent developments in the fields of microscopy and flow cytometry have sparked interest in such composite molecules, whose applications range from investigating nucleus microenvironment to drug delivery into tumours. Here we provide an overview of the properties of Hoechst dyes and discuss recent developments in Hoechst-based composite probes. View Full-Text
Keywords: fluorescent probes; biosensors; fluorescent indicators; DNA staining; protein labelling; optical microscopy; proteomics; living cells fluorescent probes; biosensors; fluorescent indicators; DNA staining; protein labelling; optical microscopy; proteomics; living cells
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Bucevičius, J.; Lukinavičius, G.; Gerasimaitė, R. The Use of Hoechst Dyes for DNA Staining and Beyond. Chemosensors 2018, 6, 18.

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