Special Issue "Fluorescent Probes for Live Cell Imaging"
A special issue of Chemosensors (ISSN 2227-9040).
Deadline for manuscript submissions: closed (31 August 2018)
Dr. Zhijie Chen
University of California, Berkeley, CA, United States
Website | E-Mail
Interests: genetically encoded fluorescent probes; hydrogen sulfide; peroxynitrite; zinc; redox biology; unnatural amino acids; live cell imaging; enzyme catalysis; single molecule biophysics; optical tweezers; transcription; epigenetics
Fluorescent probes are essential molecular tools for the detection and imaging of biological molecules. The availability of robust probes for a particular molecular species often catalyzes unprecedented advancements of an entire field, as exemplified by genetically encoded calcium indicators. In order to understand cell physiology at a molecular level, we need fluorescent probes for each cellular component such as metal ions, metabolites, lipids, proteins and nucleic acids. These fluorescent probes when coupled with live cell imaging, empower the study of biological molecules noninvasively and in its native environment.
Over the last decade, many new fluorescence imaging methods have emerged allowing for the observation of whole body processes and molecular processes in living organisms. For example, techniques in whole body and thick tissue fluorescence imaging are quickly expanding because of their potential surgical applications. Camera image-guided techniques augment surgeons’ vision. The miniaturization of flow cytometry devices has brought fluorescence imaging to the point-of-care level. Finally, super-resolution microscopy, or nanoscopy, allows objects to be observed at the nanometre (nm, 10−9 m) scale at resolutions that are only slightly lower than with electron microscopy. The importance of this discovery is emphasized by 2014 Nobel Prize in Chemistry award.
All above mentioned techniques rely on and facilitate the development of the fluorescent probes and sensors. A special attention is payed to the molecules compatible with living cell imaging allowing vizualization of dynamics, transformations and localization. This Special Issue is focusing on such fluorescent probes and attempts to build a roadmap, which, ideally, should provide useful guidelines for future developments in this vast and dynamic field.
Dr. Zhijie Chen
Dr. Grazvydas Lukinavicius
Manuscript Submission Information
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- Fluorescent probes
- Fluorescence imaging
- Molecular imaging
- Fluorescent indicators
- Live cell imaging
- Whole body and thick tissue fluorescence imaging
- Camera image-guided surgery
- Super-resolution microscopy
- Small molecules
- Genetically encoded fluorescent probes
- Chemical probes
- Cell signaling