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Volume 8
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10.3390/plants8110474
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Article

Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai (Brassica Campestris L.)

by
Lingyun Yuan
1,2,3,†,
Shilei Xie
1,2,†,
Libing Nie
1,2,
Yushan Zheng
1,2,
Jie Wang
1,2,
Ju Huang
1,
Mengru Zhao
1,2,
Shidong Zhu
1,2,3,
Jinfeng Hou
1,2,3,
Guohu Chen
1,2,3 and
Chenggang Wang
1,2,3,*
1
Vegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, China
2
Provincial Engineering Laboratory for Horticultural Crop Breeding of Anhui, Hefei 230036, China
3
Wanjiang Vegetable Industrial Technology Institute, Maanshan 238200, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Plants 2019, 8(11), 474; https://doi.org/10.3390/plants8110474
Submission received: 19 September 2019 / Revised: 30 October 2019 / Accepted: 30 October 2019 / Published: 6 November 2019
(This article belongs to the Section Plant Physiology and Metabolism)
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Abstract

Limited information is available on the cold acclimation of non-heading Chinese cabbage (NHCC) under low temperatures. In this study, the isobaric tags for relative and absolute quantification (iTRAQ) were used to illustrate the molecular machinery of cold acclimation. Compared to the control (Cont), altogether, 89 differentially expressed proteins (DEPs) were identified in wucai leaves responding to low temperatures (LT). Among these proteins, 35 proteins were up-regulated ((and 54 were down-regulated). These differentially expressed proteins were categorized as having roles in carbohydrate metabolism, photosynthesis and energy metabolism, oxidative defense, amino acid metabolism, metabolic progress, cold regulation, methylation progress, and signal transduction. The fructose, glucose, and sucrose were dramatically increased in response to cold acclimation. It was firstly reported that aspartate, serine, glutamate, proline, and threonine were significantly accumulated under low temperatures. Results of quantitative real-time PCR analysis of nine DEPs displayed that the transcriptional expression patterns of six genes were consistent with their protein expression abundance. Our results demonstrated that wucai acclimated to low temperatures through regulating the expression of several crucial proteins. Additionally, carbohydrate and amino acid conversion played indispensable and vital roles in improving cold assimilation in wucai.
Keywords: Brassica campestris; cold acclimation; proteomics; carbohydrate metabolism; amino acid Brassica campestris; cold acclimation; proteomics; carbohydrate metabolism; amino acid

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MDPI and ACS Style

Yuan, L.; Xie, S.; Nie, L.; Zheng, Y.; Wang, J.; Huang, J.; Zhao, M.; Zhu, S.; Hou, J.; Chen, G.; et al. Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai (Brassica Campestris L.). Plants 2019, 8, 474. https://doi.org/10.3390/plants8110474

AMA Style

Yuan L, Xie S, Nie L, Zheng Y, Wang J, Huang J, Zhao M, Zhu S, Hou J, Chen G, et al. Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai (Brassica Campestris L.). Plants. 2019; 8(11):474. https://doi.org/10.3390/plants8110474

Chicago/Turabian Style

Yuan, Lingyun, Shilei Xie, Libing Nie, Yushan Zheng, Jie Wang, Ju Huang, Mengru Zhao, Shidong Zhu, Jinfeng Hou, Guohu Chen, and et al. 2019. "Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai (Brassica Campestris L.)" Plants 8, no. 11: 474. https://doi.org/10.3390/plants8110474

APA Style

Yuan, L., Xie, S., Nie, L., Zheng, Y., Wang, J., Huang, J., Zhao, M., Zhu, S., Hou, J., Chen, G., & Wang, C. (2019). Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai (Brassica Campestris L.). Plants, 8(11), 474. https://doi.org/10.3390/plants8110474

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