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Volume 12
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10.3390/plants12223916
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Article

A Novel Non-Specific Lipid Transfer Protein Gene, CmnsLTP6.9, Enhanced Osmotic and Drought Tolerance by Regulating ROS Scavenging and Remodeling Lipid Profiles in Chinese Chestnut (Castanea mollissima Blume)

by
Yuxiong Xiao
†,
Cui Xiao
†,
Xiujuan He
†,
Xin Yang
,
Zhu Tong
,
Zeqiong Wang
,
Zhonghai Sun
and
Wenming Qiu
*
Hubei Key Laboratory of Germplasm Innovation and Utilization of Fruit Trees, Institute of Fruit and Tea, Hubei Academy of Agricultural Sciences, Wuhan 430064, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Plants 2023, 12(22), 3916; https://doi.org/10.3390/plants12223916
Submission received: 15 September 2023 / Revised: 17 November 2023 / Accepted: 18 November 2023 / Published: 20 November 2023
(This article belongs to the Special Issue Molecular Responses to Abiotic Stresses and Signaling in Horticultural Plants)
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Abstract

Chestnut (Castanea mollissima Blume) is an important economic tree owing to its tasty fruit and adaptability to environmental stresses, especially drought. Currently, there is limited information about non-specific lipid transfer protein (nsLTP) genes that respond to abiotic stress in chestnuts. Here, a chestnut nsLTP, named CmnsLTP6.9, was identified and analyzed. The results showed that the CmnsLTP6.9 protein localized in the extracellular matrix had two splicing variants (CmnsLTP6.9L and CmnsLTP6.9S). Compared with CmnsLTP6.9L, CmnsLTP6.9S had an 87 bp deletion in the 5′-terminal. Overexpression of CmnsLTP6.9L in Arabidopsis enhanced tolerance to osmotic and drought stress. Upon exposure to osmotic and drought treatment, CmnsLTP6.9L could increase reactive oxygen species (ROS)-scavenging enzyme activity, alleviating ROS damage. However, CmnsLTP6.9S-overexpressing lines showed no significant differences in phenotype, ROS content, and related enzyme activities compared with the wild type (WT) under osmotic and drought treatment. Moreover, lipid metabolism analysis confirmed that, unlike CmnsLTP6.9S, CmnsLTP6.9L mainly altered and upregulated many fatty acyls and glycerophospholipids, which implied that CmnsLTP6.9L and CmnsLTP6.9S played different roles in lipid transference in the chestnut. Taken together, we analyzed the functions of CmnsLTP6.9L and CmnsLTP6.9S, and demonstrated that CmnsLTP6.9L enhanced drought and osmotic stress tolerance through ROS scavenging and lipid metabolism.
Keywords: chestnut; CmnsLTP; CmnsLTP6.9; osmotic; drought chestnut; CmnsLTP; CmnsLTP6.9; osmotic; drought

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MDPI and ACS Style

Xiao, Y.; Xiao, C.; He, X.; Yang, X.; Tong, Z.; Wang, Z.; Sun, Z.; Qiu, W. A Novel Non-Specific Lipid Transfer Protein Gene, CmnsLTP6.9, Enhanced Osmotic and Drought Tolerance by Regulating ROS Scavenging and Remodeling Lipid Profiles in Chinese Chestnut (Castanea mollissima Blume). Plants 2023, 12, 3916. https://doi.org/10.3390/plants12223916

AMA Style

Xiao Y, Xiao C, He X, Yang X, Tong Z, Wang Z, Sun Z, Qiu W. A Novel Non-Specific Lipid Transfer Protein Gene, CmnsLTP6.9, Enhanced Osmotic and Drought Tolerance by Regulating ROS Scavenging and Remodeling Lipid Profiles in Chinese Chestnut (Castanea mollissima Blume). Plants. 2023; 12(22):3916. https://doi.org/10.3390/plants12223916

Chicago/Turabian Style

Xiao, Yuxiong, Cui Xiao, Xiujuan He, Xin Yang, Zhu Tong, Zeqiong Wang, Zhonghai Sun, and Wenming Qiu. 2023. "A Novel Non-Specific Lipid Transfer Protein Gene, CmnsLTP6.9, Enhanced Osmotic and Drought Tolerance by Regulating ROS Scavenging and Remodeling Lipid Profiles in Chinese Chestnut (Castanea mollissima Blume)" Plants 12, no. 22: 3916. https://doi.org/10.3390/plants12223916

APA Style

Xiao, Y., Xiao, C., He, X., Yang, X., Tong, Z., Wang, Z., Sun, Z., & Qiu, W. (2023). A Novel Non-Specific Lipid Transfer Protein Gene, CmnsLTP6.9, Enhanced Osmotic and Drought Tolerance by Regulating ROS Scavenging and Remodeling Lipid Profiles in Chinese Chestnut (Castanea mollissima Blume). Plants, 12(22), 3916. https://doi.org/10.3390/plants12223916

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