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Article

A Cell-Free SDKP-Conjugated Self-Assembling Peptide Hydrogel Sufficient for Improvement of Myocardial Infarction

1
Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran 1449614535, Iran
2
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran 1665659911, Iran
3
Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran 1665659911, Iran
4
Research Center for Advanced Technologies in Cardiovascular Medicine, Tehran Heart Center, Tehran University of Medical Sciences, Tehran 1416753955, Iran
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Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran 1449614535, Iran
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Department of Regenerative Medicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran 1665659911, Iran
7
Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
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Department of Developmental Biology, University of Science and Culture, ACECR, Tehran 1461968151, Iran
*
Authors to whom correspondence should be addressed.
Biomolecules 2020, 10(2), 205; https://doi.org/10.3390/biom10020205
Received: 4 December 2019 / Revised: 12 January 2020 / Accepted: 25 January 2020 / Published: 30 January 2020
(This article belongs to the Special Issue Self-Assembling Peptides)
Biomaterials in conjunction with stem cell therapy have recently attracted attention as a new therapeutic approach for myocardial infarction (MI), with the aim to solve the delivery challenges that exist with transplanted cells. Self-assembling peptide (SAP) hydrogels comprise a promising class of synthetic biomaterials with cardiac-compatible properties such as mild gelation, injectability, rehealing ability, and potential for sequence modification. Herein, we developed an SAP hydrogel composed of a self-assembling gel-forming core sequence (RADA) modified with SDKP motif with pro-angiogenic and anti-fibrotic activity to be used as a cardioprotective scaffold. The RADA-SDKP hydrogel was intramyocardially injected into the infarct border zone of a rat model of MI induced by left anterior descending artery (LAD) ligation as a cell-free or a cell-delivering scaffold for bone marrow mesenchymal stem cells (BM-MSCs). The left ventricular ejection fraction (LVEF) was markedly improved after transplantation of either free hydrogel or cell-laden hydrogel. This cardiac functional repair coincided very well with substantially lower fibrotic tissue formation, expanded microvasculature, and lower inflammatory response in the infarct area. Interestingly, BM-MSCs alone or in combination with hydrogel could not surpass the cardiac repair effects of the SDKP-modified SAP hydrogel. Taken together, we suggest that the RADA-SDKP hydrogel can be a promising cell-free construct that has the capability for functional restoration in the instances of acute myocardial infarction (AMI) that might minimize the safety concerns of cardiac cell therapy and facilitate clinical extrapolation. View Full-Text
Keywords: mesenchymal stem cells; cardiac cell therapy; self-assembling peptide hydrogel; myocardial infarction mesenchymal stem cells; cardiac cell therapy; self-assembling peptide hydrogel; myocardial infarction
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MDPI and ACS Style

Firoozi, S.; Pahlavan, S.; Ghanian, M.-H.; Rabbani, S.; Tavakol, S.; Barekat, M.; Yakhkeshi, S.; Mahmoudi, E.; Soleymani, M.; Baharvand, H. A Cell-Free SDKP-Conjugated Self-Assembling Peptide Hydrogel Sufficient for Improvement of Myocardial Infarction. Biomolecules 2020, 10, 205. https://doi.org/10.3390/biom10020205

AMA Style

Firoozi S, Pahlavan S, Ghanian M-H, Rabbani S, Tavakol S, Barekat M, Yakhkeshi S, Mahmoudi E, Soleymani M, Baharvand H. A Cell-Free SDKP-Conjugated Self-Assembling Peptide Hydrogel Sufficient for Improvement of Myocardial Infarction. Biomolecules. 2020; 10(2):205. https://doi.org/10.3390/biom10020205

Chicago/Turabian Style

Firoozi, Saman, Sara Pahlavan, Mohammad-Hossein Ghanian, Shahram Rabbani, Shima Tavakol, Maryam Barekat, Saeed Yakhkeshi, Elena Mahmoudi, Mansoureh Soleymani, and Hossein Baharvand. 2020. "A Cell-Free SDKP-Conjugated Self-Assembling Peptide Hydrogel Sufficient for Improvement of Myocardial Infarction" Biomolecules 10, no. 2: 205. https://doi.org/10.3390/biom10020205

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