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Structural Characteristics and Hypolipidemic Activity of Theabrownins from Dark Tea Fermented by Single Species Eurotium cristatum PW-1

by 1,2, 1,2, 1,2, 1,2,* and 1,2
1
College of Biomass Science and Engineering and Healthy Food Evaluation Research Center, Sichuan University, Chengdu 610065, China
2
Key Laboratory of Food Science and Technology of Ministry of Education of Sichuan Province, Sichuan University, Chengdu 610065, China
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(2), 204; https://doi.org/10.3390/biom10020204
Received: 18 December 2019 / Revised: 22 January 2020 / Accepted: 27 January 2020 / Published: 30 January 2020
Recently, studies on theabrownins (TBs), the main bioactive polymeric pigments found in dark tea, have received increasing attention for its health effects. Thus far, information on their structural characteristics is unclear. In the present study, theabrownins were isolated from single species Eurotium cristatum PW-1-fermented loose tea and their structural and hypolipidemic characteristics were studied for the first time. The theabrownins were fractionated by their molecular weights and were then analyzed. Ultraviolet–visible spectrophotometry (UV-Vis) and Flourier transformation infrared spectroscopy (FT-IR) showed that they were polymerized phenolic substances containing abundant hydroxy and carboxyl groups. All theabrownin samples exhibited hypolipidemic activity in high-fat zebrafish; among which TBs-10-30k sample, decreased lipid level in high-fat zebrafish to 51.57% at 1000 μg/mL, was most effective. It was found that TBs-10-30k was a type of amorphous and thermostable polymer with slice shape and smooth surface under scanning electron microscope (SEM). Atomic force microscope (AFM) analysis showed that it had island-like structure because of aggregation of theabrownin molecules. Pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) analysis further showed that the main pyrolytic products of TBs-10-30k were hexadecanoic acid (33.72%), phenol (14.90%), and eicosane (12.95%), indicating TBs-10-30k was mainly composed of phenols, lipids, saccharides, and proteins. These results not only facilitate subsequent identification of theabrownins, but also provide insights into the applications of theabrownins in functional foods. View Full-Text
Keywords: theabrownins; fractionated; E. cristatum fermented loose tea; structural characteristics; hypolipidemic activity theabrownins; fractionated; E. cristatum fermented loose tea; structural characteristics; hypolipidemic activity
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MDPI and ACS Style

Xiao, Y.; Li, M.; Wu, Y.; Zhong, K.; Gao, H. Structural Characteristics and Hypolipidemic Activity of Theabrownins from Dark Tea Fermented by Single Species Eurotium cristatum PW-1. Biomolecules 2020, 10, 204. https://doi.org/10.3390/biom10020204

AMA Style

Xiao Y, Li M, Wu Y, Zhong K, Gao H. Structural Characteristics and Hypolipidemic Activity of Theabrownins from Dark Tea Fermented by Single Species Eurotium cristatum PW-1. Biomolecules. 2020; 10(2):204. https://doi.org/10.3390/biom10020204

Chicago/Turabian Style

Xiao, Yue, Maoyun Li, Yanping Wu, Kai Zhong, and Hong Gao. 2020. "Structural Characteristics and Hypolipidemic Activity of Theabrownins from Dark Tea Fermented by Single Species Eurotium cristatum PW-1" Biomolecules 10, no. 2: 204. https://doi.org/10.3390/biom10020204

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