Quadruplex-Forming Motif Inserted into 3′UTR of Ty1his3-AI Retrotransposon Inhibits Retrotransposition in Yeast
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Plasmid Construction
2.2. Circular Dichroism Spectroscopy, PAGE
2.3. UV Absorption Spectroscopy and Thermal Melting
2.4. Retromobility Assay
2.5. RT-qPCR
2.6. cDNA Analysis
3. Results
3.1. Characterization and Biophysical Properties of G4 Sequences
3.2. Inhibition of Retrotransposition—Effect of Strand Orientation, Pif1 Deficiency and NMM
3.3. The Effect of G4 and Its Stabilization on mRNA Levels and Products of Reverse Transcription
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Tokan, V.; Rodriguez Lorenzo, J.L.; Jedlicka, P.; Kejnovska, I.; Hobza, R.; Kejnovsky, E. Quadruplex-Forming Motif Inserted into 3′UTR of Ty1his3-AI Retrotransposon Inhibits Retrotransposition in Yeast. Biology 2021, 10, 347. https://doi.org/10.3390/biology10040347
Tokan V, Rodriguez Lorenzo JL, Jedlicka P, Kejnovska I, Hobza R, Kejnovsky E. Quadruplex-Forming Motif Inserted into 3′UTR of Ty1his3-AI Retrotransposon Inhibits Retrotransposition in Yeast. Biology. 2021; 10(4):347. https://doi.org/10.3390/biology10040347
Chicago/Turabian StyleTokan, Viktor, Jose Luis Rodriguez Lorenzo, Pavel Jedlicka, Iva Kejnovska, Roman Hobza, and Eduard Kejnovsky. 2021. "Quadruplex-Forming Motif Inserted into 3′UTR of Ty1his3-AI Retrotransposon Inhibits Retrotransposition in Yeast" Biology 10, no. 4: 347. https://doi.org/10.3390/biology10040347
APA StyleTokan, V., Rodriguez Lorenzo, J. L., Jedlicka, P., Kejnovska, I., Hobza, R., & Kejnovsky, E. (2021). Quadruplex-Forming Motif Inserted into 3′UTR of Ty1his3-AI Retrotransposon Inhibits Retrotransposition in Yeast. Biology, 10(4), 347. https://doi.org/10.3390/biology10040347