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Open AccessArticle

Detection of Breast Cancer Cells Using Acoustics Aptasensor Specific to HER2 Receptors

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Institute of Animal Biochemistry and Genetics, Center of Biosciences SAS, Dúbravská cesta 9, 840 05 Bratislava, Slovakia
2
Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynská dolina F1, 842 48 Bratislava, Slovakia
3
Cancer Research Institute, Biomedical Research Center SAS, Dúbravská cesta 9, 840 05 Bratislava, Slovakia
*
Author to whom correspondence should be addressed.
Biosensors 2019, 9(2), 72; https://doi.org/10.3390/bios9020072
Received: 23 April 2019 / Revised: 17 May 2019 / Accepted: 23 May 2019 / Published: 27 May 2019
(This article belongs to the Special Issue Feature Papers: State-of-the-Art Biosensors Technology 2018)
Detection of the breast cancer cells is important for early diagnosis of the cancer. We applied thickness shear mode acoustics method (TSM) for detection of SK-BR-3 breast cancer cells using DNA aptamers specific to HER2 positive membrane receptors. The biotinylated aptamers were immobilized at the neutravidin layer chemisorbed at gold surface of TSM transducer. Addition of the cells resulted in decrease of resonant frequency, fs, and in increase of motional resistance, Rm. Using gold nanoparticles (AuNPs), modified by aptamers it was possible improving the limit of detection (LOD) that reached 550 cells/mL, while without amplification the sensitivity of the detection of SK-BR-3 cells was 1574 cells/mL. HER2 negative cell line MDA-MB-231 did not resulted in significant changes of fs. The viability studies demonstrated that cells are stable at experimental conditions used during at least 8 h. AuNPs were not toxic on the cells up to concentration of 1 μg/mL. View Full-Text
Keywords: breast cancer cells; HER2 receptors; DNA aptamer; acoustic biosensor; gold nanoparticles breast cancer cells; HER2 receptors; DNA aptamer; acoustic biosensor; gold nanoparticles
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Poturnayová, A.; Dzubinová, Ľ.; Buríková, M.; Bízik, J.; Hianik, T. Detection of Breast Cancer Cells Using Acoustics Aptasensor Specific to HER2 Receptors. Biosensors 2019, 9, 72.

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