Creating Supported Plasma Membrane Bilayers Using Acoustic Pressure

Sezgin, Erdinc; Carugo, Dario; Levental, Ilya; Stride, Eleanor; Eggeling, Christian

doi:10.3390/membranes10020030

Open AccessBrief Report

Creating Supported Plasma Membrane Bilayers Using Acoustic Pressure

by

Erdinc Sezgin

^1,2,*

,

Dario Carugo

³,

Ilya Levental

⁴,

Eleanor Stride

⁵

and

Christian Eggeling

^1,6,7,*

¹

MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, UK

²

Science for Life Laboratory, Department of Women’s and Children’s Health, Karolinska Institutet, 171 65 Stockholm, Sweden

³

Bioengineering Science Research Group, Faculty of Engineering and Physical Sciences, Institute for Life Sciences (IfLS), University of Southampton, SO17 1BJ Southampton, UK

⁴

McGovern Medical School, Department of Integrative Biology and Pharmacology, University of Texas Health Science Center at Houston, Houston, TX 77030, USA

⁵

Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford OX3 7DQ, UK

⁶

Institute of Applied Optics and Biophysics, Friedrich-Schiller-University Jena, Max-Wien Platz 4, 07743 Jena, Germany

⁷

Leibniz Institute of Photonic Technology e.V., Albert-Einstein-Straße 9, 07745 Jena, Germany

^*

Authors to whom correspondence should be addressed.

Membranes 2020, 10(2), 30; https://doi.org/10.3390/membranes10020030

Submission received: 23 January 2020 / Revised: 12 February 2020 / Accepted: 14 February 2020 / Published: 18 February 2020

(This article belongs to the Special Issue Dynamics and Nano-Organization in Plasma Membranes)

Download

Browse Figures

Review Reports Versions Notes

Abstract

Model membrane systems are essential tools for the study of biological processes in a simplified setting to reveal the underlying physicochemical principles. As cell-derived membrane systems, giant plasma membrane vesicles (GPMVs) constitute an intermediate model between live cells and fully artificial structures. Certain applications, however, require planar membrane surfaces. Here, we report a new approach for creating supported plasma membrane bilayers (SPMBs) by bursting cell-derived GPMVs using ultrasound within a microfluidic device. We show that the mobility of outer leaflet molecules is preserved in SPMBs, suggesting that they are accessible on the surface of the bilayers. Such model membrane systems are potentially useful in many applications requiring detailed characterization of plasma membrane dynamics.

Keywords: GPMVs; acoustic pressure; supported bilayers; plasma membrane vesicles; plasma membrane bilayers

Share and Cite

MDPI and ACS Style

Sezgin, E.; Carugo, D.; Levental, I.; Stride, E.; Eggeling, C. Creating Supported Plasma Membrane Bilayers Using Acoustic Pressure. Membranes 2020, 10, 30. https://doi.org/10.3390/membranes10020030

AMA Style

Sezgin E, Carugo D, Levental I, Stride E, Eggeling C. Creating Supported Plasma Membrane Bilayers Using Acoustic Pressure. Membranes. 2020; 10(2):30. https://doi.org/10.3390/membranes10020030

Chicago/Turabian Style

Sezgin, Erdinc, Dario Carugo, Ilya Levental, Eleanor Stride, and Christian Eggeling. 2020. "Creating Supported Plasma Membrane Bilayers Using Acoustic Pressure" Membranes 10, no. 2: 30. https://doi.org/10.3390/membranes10020030

APA Style

Sezgin, E., Carugo, D., Levental, I., Stride, E., & Eggeling, C. (2020). Creating Supported Plasma Membrane Bilayers Using Acoustic Pressure. Membranes, 10(2), 30. https://doi.org/10.3390/membranes10020030

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Menu

Creating Supported Plasma Membrane Bilayers Using Acoustic Pressure

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI