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Open AccessArticle

Evaluation of Three Different Vaccination Protocols against EHV1/EHV4 Infection in Mares: Double Blind, Randomized Clinical Trial

1
School of Biosciences and Veterinary Medicine, University of Camerino, 62024 Matelica, Italy
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Biologist, Senior Consultant, 20027 Ispra, Italy
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Veterinary Practitioner, 60010 Ostra, Italy
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Veterinary Practitioner, 30028 San Michele al Tagliamento, Italy
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Istituto Zooprofilattico Sperimentale Umbria e Marche “Togo Rosati”, 06126 Perugia, Italy
*
Author to whom correspondence should be addressed.
Vaccines 2020, 8(2), 268; https://doi.org/10.3390/vaccines8020268
Received: 12 April 2020 / Accepted: 27 May 2020 / Published: 1 June 2020
(This article belongs to the Section Veterinary Vaccines)
EHV1 and EHV4 are the most important herpesviruses in horses. Repeated cases of abortion in mares regularly vaccinated, prompted us to investigate the immune response after vaccination with the same inactivated vaccine, but with three different protocols. Eighteen mares were chosen and randomly divided in three study groups (G1-G2-G3) and a control group (Ctrl). For serologic and PCR investigations nasal swabs, sera and blood were collected. The protocol used in G3 (4 doses) increased the titer recorded by ELISA and seroneutralization (SN). Poor agreement and no correlation were observed in titer values between ELISA and SN and between SN and PCR. A very weak positive correlation between ELISA and PCR was obtained. Seven out of 18 nasal swabs were positive by PCR; none showed viremia and no abortion occurred, regardless of vaccination status and despite active circulation of EHV-1 in the farm at the time of the study. The study was conducted in field conditions, in a susceptible population with a known history of infection and abortion, and among the three protocols, the one proposed in the G1 was the least efficient while the one proposed for the G3, seems to have induced a higher antibody titer in both SN and ELISA. View Full-Text
Keywords: horses; vaccines; herpesvirus; EHV1; EHV4; abortion; PCR; seroneutralization; ELISA horses; vaccines; herpesvirus; EHV1; EHV4; abortion; PCR; seroneutralization; ELISA
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MDPI and ACS Style

Attili, A.-R.; Colognato, R.; Preziuso, S.; Moriconi, M.; Valentini, S.; Petrini, S.; De Mia, G.M.; Cuteri, V. Evaluation of Three Different Vaccination Protocols against EHV1/EHV4 Infection in Mares: Double Blind, Randomized Clinical Trial. Vaccines 2020, 8, 268.

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