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Chemopreventive Effect of β-Cryptoxanthin on Human Cervical Carcinoma (HeLa) Cells Is Modulated through Oxidative Stress-Induced Apoptosis

1
Department of Bioresources and Food Science, Konkuk University, Seoul 143-701, Korea
2
Institute of Natural Science and Agriculture, Konkuk University, Seoul 143-701, Korea
3
Department of Crop Science, Konkuk University, Seoul 143-701, Korea
*
Author to whom correspondence should be addressed.
Antioxidants 2020, 9(1), 28; https://doi.org/10.3390/antiox9010028
Received: 25 November 2019 / Revised: 19 December 2019 / Accepted: 25 December 2019 / Published: 27 December 2019
(This article belongs to the Special Issue Carotenoids, Oxidative Stress and Disease)
The present study was aimed to assess cellular and molecular events involved in the chemopreventive activities of β-cryptoxanthin derived from mandarin oranges (Citrus unshiu Marc.) on human cervical carcinoma (HeLa) cells. In vitro experiments established that β-cryptoxanthin significantly inhibited the proliferation of HeLa cells with the IC50 value of 4.5 and 3.7 µM after 24 and 48 h of treatments, respectively. β-cryptoxanthin-treated HeLa cells exhibited enhanced levels of oxidative stress correlated with significant downregulation of anti-apoptotic Bcl-2, and upregulation of pro-apoptotic Bax mRNA expression. Moreover, β-cryptoxanthin triggered nuclear condensation and disruption of the integrity of the mitochondrial membrane, upregulated caspase-3, -7, and -9 mRNA, and enhanced activation of caspase-3 proteins, resulting in nuclei DNA damage and apoptosis of HeLa cells. Remarkably, TUNEL assay carried out to detect nuclei DNA damage showed 52% TUNEL-positive cells after treatment with a physiological concentration of β-cryptoxanthin (1.0 μM), which validates its potential as an anticancer drug of natural origin. View Full-Text
Keywords: carotenoids; DNA fragmentation; mitochondrial membrane potential; ROS-induced apoptosis; TUNEL assay carotenoids; DNA fragmentation; mitochondrial membrane potential; ROS-induced apoptosis; TUNEL assay
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MDPI and ACS Style

Gansukh, E.; Nile, A.; Sivanesan, I.; Rengasamy, K.R.R.; Kim, D.-H.; Keum, Y.-S.; Saini, R.K. Chemopreventive Effect of β-Cryptoxanthin on Human Cervical Carcinoma (HeLa) Cells Is Modulated through Oxidative Stress-Induced Apoptosis. Antioxidants 2020, 9, 28.

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