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Antioxidant Activities of Sorghum Kafirin Alcalase Hydrolysates and Membrane/Gel Filtrated Fractions

Department of Grain Science and Industry, Kansas State University, Manhattan, KS 66506, USA
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Antioxidants 2019, 8(5), 131; https://doi.org/10.3390/antiox8050131
Received: 9 April 2019 / Revised: 7 May 2019 / Accepted: 13 May 2019 / Published: 15 May 2019
(This article belongs to the Special Issue Extraction of Antioxidants from Food Waste)
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Abstract

Sorghum has a significant amount of proteins, especially kafirin; however, limited information is available on evaluating its potential for peptide antioxidants. The objectives of this study were to: (1) investigate the effects of two key variables, enzyme-to-substrate ratio and reaction time on kafirin hydrolysis using Alcalase; (2) evaluate the antioxidant performances of the hydrolysates and fractions from membrane ultrafiltration and gel filtration; and (3) identify peptide sequences in the antioxidant fraction using MALDI-TOF/TOF MS. Kafirin hydrolysates prepared at enzyme-to-substrate ratio of 0.4 Au/g and 4 h had a good balance of antioxidant activity, yield, and economic efficiency. Medium-sized fraction of hydrolysates (5–10 kDa) from membrane filtration possessed the highest antioxidant activities among various fractions. The fraction also unveiled a good inhibition effect against lipid oxidation in emulsion and ground meat systems. Smaller-sized fraction (F3) collected through gel-filtration chromatography had significantly stronger antioxidant activities than other fractions, and 26 representative peptide sequences were identified in the fraction. View Full-Text
Keywords: grain sorghum; kafirin; protein hydrolysate; antioxidant; emulsion; ground meat; peptide sequence grain sorghum; kafirin; protein hydrolysate; antioxidant; emulsion; ground meat; peptide sequence
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Xu, S.; Shen, Y.; Li, Y. Antioxidant Activities of Sorghum Kafirin Alcalase Hydrolysates and Membrane/Gel Filtrated Fractions. Antioxidants 2019, 8, 131.

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