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Article

Antioxidant Activities of Sorghum Kafirin Alcalase Hydrolysates and Membrane/Gel Filtrated Fractions

Department of Grain Science and Industry, Kansas State University, Manhattan, KS 66506, USA
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Antioxidants 2019, 8(5), 131; https://doi.org/10.3390/antiox8050131
Received: 9 April 2019 / Revised: 7 May 2019 / Accepted: 13 May 2019 / Published: 15 May 2019
(This article belongs to the Special Issue Extraction of Antioxidants from Food Waste)
Sorghum has a significant amount of proteins, especially kafirin; however, limited information is available on evaluating its potential for peptide antioxidants. The objectives of this study were to: (1) investigate the effects of two key variables, enzyme-to-substrate ratio and reaction time on kafirin hydrolysis using Alcalase; (2) evaluate the antioxidant performances of the hydrolysates and fractions from membrane ultrafiltration and gel filtration; and (3) identify peptide sequences in the antioxidant fraction using MALDI-TOF/TOF MS. Kafirin hydrolysates prepared at enzyme-to-substrate ratio of 0.4 Au/g and 4 h had a good balance of antioxidant activity, yield, and economic efficiency. Medium-sized fraction of hydrolysates (5–10 kDa) from membrane filtration possessed the highest antioxidant activities among various fractions. The fraction also unveiled a good inhibition effect against lipid oxidation in emulsion and ground meat systems. Smaller-sized fraction (F3) collected through gel-filtration chromatography had significantly stronger antioxidant activities than other fractions, and 26 representative peptide sequences were identified in the fraction. View Full-Text
Keywords: grain sorghum; kafirin; protein hydrolysate; antioxidant; emulsion; ground meat; peptide sequence grain sorghum; kafirin; protein hydrolysate; antioxidant; emulsion; ground meat; peptide sequence
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MDPI and ACS Style

Xu, S.; Shen, Y.; Li, Y. Antioxidant Activities of Sorghum Kafirin Alcalase Hydrolysates and Membrane/Gel Filtrated Fractions. Antioxidants 2019, 8, 131. https://doi.org/10.3390/antiox8050131

AMA Style

Xu S, Shen Y, Li Y. Antioxidant Activities of Sorghum Kafirin Alcalase Hydrolysates and Membrane/Gel Filtrated Fractions. Antioxidants. 2019; 8(5):131. https://doi.org/10.3390/antiox8050131

Chicago/Turabian Style

Xu, Shiwei, Yanting Shen, and Yonghui Li. 2019. "Antioxidant Activities of Sorghum Kafirin Alcalase Hydrolysates and Membrane/Gel Filtrated Fractions" Antioxidants 8, no. 5: 131. https://doi.org/10.3390/antiox8050131

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