Search Results (5,092)

Cartilage regeneration remains an unmet clinical challenge. Despite the great advances in the production of hydrogels as support matrices for cartilage regeneration, the resulting mechanical properties remain low. Granular composite hydrogels appear as ideal candidates due to their injectability and modularity in design. Here, we report on the fabrication and characterization of heterogeneous composite granular hydrogels based on methacrylated chitosan (CHIMA) and gelatin (GelMA) microparticles supported by an interstitial methacrylated alginate (ALMA) matrix. Microparticles were prepared by an oil-emulsion method and their size and morphology optimized, resulting in CHIMA and GelMA microparticles of 10.8 µm (95% CI 9.2, 13.1) and 115.8 µm (95% CI 107.5, 137.6) in diameter, respectively. The microparticles were mixed with ALMA and crosslinked to form granular hydrogels that demonstrated reduced swelling and weight loss. The storage modulus increased from 33 to 66.4 kPa for CHIMA/ALMA hydrogels and from 11.5 to 19.5 kPa for GelMA/ALMA hydrogels when the particle concentration increased from 10 to 50%, and was higher than traditional ALMA hydrogels. Hydrogels of 50:50 CHIMA:GelMA permitted a 6.6-fold increase in cell number after 28 days of culture, and promoted the chondrogenic differentiation of embedded mouse mesenchymal stem cells with a glycosaminoglycan deposition of over 15 µg and the expression of chondrogenic markers. Full article

β-Carotene is widely utilized in food systems due to its biological activities, but exhibits poor chemical stability and low bioavailability. This study utilized whole Auricularia polytricha (Mont.) Sacc. powder as a direct emulsifier to establish a natural emulsion-based delivery system designed to enhance the stability of β-carotene. Under optimal conditions, using 7% Auricularia polytricha (Mont.) Sacc. powder (120 μm) and 1% oil phase fraction, microscopic analysis revealed that emulsion droplets were small and uniformly distributed, resulting in excellent long-term stability. After UV irradiation, the degradation rate of β-carotene in the emulsion was significantly lower than that of β-carotene directly dispersed in the oil phase. In vitro simulated digestion indicated that β-carotene retention in the intestinal phase reached 9.2% in the emulsion system, 1.2 ± 0.23% higher than in the conventional oil-dissolved system. This strategy offers a practical approach for the high-value utilization of this fungal resource, streamlining industrial processes and reducing production costs. Full article

This study employs a Computational Fluid Dynamics (CFD) approach based on the Two-Fluid Model (TFM) to investigate the CO₂ capture characteristics in a bubbling fluidized bed reactor using potassium carbonate (K₂CO₃) as the sorbent. The simulations are conducted at five superficial gas velocities ranging from 1.5 to 3.5 times the minimum bubbling velocity (u_mb = 0.26 m/s), with a particle diameter of 0.4 mm, particle density of 2300 kg/m³, and an initial solid volume fraction of 0.55. The gas mixture consists of CO₂, H₂O, and N₂ at a molar ratio of 0.1:0.1:0.8 and a temperature of 343 K. First, the numerical simulation was validated against experimental data reported in the literature, confirming its accuracy in quantitatively describing the adsorption process. Subsequently, the distributions of CO₂ concentration and adsorption reaction rate in both the bubble phase and the emulsion phase were analyzed under different superficial gas velocities. The simulation results indicate that CO₂ concentration and adsorption reaction rate in both phases decrease along the bed height. Compared to the emulsion phase, the bubble phase exhibits higher CO₂ concentration and gas temperature but a lower adsorption reaction rate. As the gas velocity increases, CO₂ concentration rises in both the bubble and emulsion phases, accompanied by an increase in the proportion of the bubble phase, and a higher CO₂ concentration at the reactor outlet. Further comparison of CO₂ concentrations in the bubble and emulsion phases at the upper part of the bed with the outlet concentration reveals that the outlet CO₂ primarily originates from the unadsorbed portion within the bubble phase, while the contribution from unadsorbed CO₂ in the emulsion phase is almost negligible. Full article

The incorporation of plant-derived oils into cosmetic formulations has attracted increasing interest due to their natural origin, skin compatibility, and multifunctional formulation roles. Argan and castor oils are widely used in cosmetic products as emollient lipid components with intrinsic antioxidant properties. However, limited studies have systematically evaluated the physicochemical stability and antioxidant performance of emulsions combining these two oils. The aim of this study was to develop and comprehensively characterize a stable oil-in-water (O/W) cosmetic emulsion based on argan and castor oils using a natural non-ionic emulsifier (C14–22 Alcohol (and) C12–20 Alkyl Glucoside). Particular emphasis was placed on formulation stability, as it represents a critical prerequisite for further product evaluation. Stability was investigated through thermal stress testing (4–37 °C), centrifugation assays, droplet size analysis, and zeta potential measurements. Complementary physicochemical and structural characterization was performed using rheological analysis and Fourier transform infrared (FT-IR) spectroscopy. The formulated emulsion exhibited good physical stability with no phase separation under the tested conditions, a skin-compatible pH, a uniform droplet size distribution (4.15 ± 0.68 µm), and pseudoplastic, moderately thixotropic rheological behavior. Antioxidant capacity was assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, yielding an IC₅₀ value of 19.21 ± 1.02 mg/mL. Overall, this study provides a formulation-oriented framework for the development and evaluation of stable natural oil-based O/W emulsions intended for cosmetic applications, supporting future optimization and biological validation. Full article

This study explored the dual chemical modification of starch isolated from red lentils (Lens culinaris) to develop a biodegradable polymer with enhanced functionality for multifaceted applications. Native starch was isolated via combined salt–alkali treatment and sequentially modified through epichlorohydrin-mediated crosslinking, followed by cationization using glycidyl trimethylammonium chloride (GTAC). Utilizing a Quality by Design (QbD) strategy through Response Surface Methodology (RSM), the cationization endured fine-tuning to reach an optimal degree of substitution (DS = 0.572) under foremost conditions (GTAC: 2.1 mol, NaOH: 0.09 mol, reaction time: 18 h). Structural and functional characterization using FTIR, XRD, TGA, SEM, and zeta potential analysis confirmed the successful modification, indicating enhanced thermal stability, a transition to a more amorphous structure, and a moderately positive surface charge (+7.24 mV). The dual modified cationic lentil starch (CLS) demonstrated effective flocculation of kaolin suspensions, achieving a transmittance of up to 94%. Additionally, CLS showed significantly improved emulsion stability, maintaining over 70% stability after 24 h, compared to native starch, which dropped below 30%. These results emphasize the promising potential of CLS as an eco-friendly and high-performance alternative to synthetic polymers for water treatment and stabilization of emulsion-based formulations. Full article

The poultry industry generates large amounts of protein- and mineral-rich by-products that remain underutilized. This study investigated the use of chicken skin protein hydrolysate and chicken bone protein–mineral mass (PMM) as functional ingredients in emulsified poultry sausages. The hydrolysate was characterized by a high protein content (52.25%) and high water- and fat-binding capacity (142% and 125%, respectively), while the PMM served as a source of protein and minerals with stable physicochemical and rheological characteristics. These ingredients were incorporated into sausage formulations at different substitution levels. Partial replacement of poultry meat increased protein and mineral content and affected key technological properties, including water-binding capacity, emulsion stability, cooking loss, and shear force. Moderate inclusion levels were associated with a more cohesive protein matrix, lower cooking losses, and improved structural stability, whereas excessive substitution resulted in increased firmness and less favorable sensory characteristics. Among the tested formulations, the combination of 18% PMM and 4% protein hydrolysate showed the most balanced technological and sensory performance. The findings suggest that poultry by-products processed into functional ingredients may have potential for application in value-added sausage formulations. Full article

Micronutrient deficiencies, particularly of vitamins A, D₃, and folic acid, remain a significant global health challenge despite established dietary recommendations. This study proposes a novel fortification strategy using advanced emulsion technology to enrich extra-virgin olive oil (EVOO) with these essential micronutrients. Water-in-oil (W/O) and double oil-in-water-in-oil (O/W/O) emulsions were designed to enable the simultaneous encapsulation of lipophilic (A and D₃) and hydrophilic (folic acid) vitamins within a single functional food matrix. Vitamin concentrations were quantified using high-performance liquid chromatography (HPLC) coupled with a photodiode detector (PDA) to evaluate retention during processing. Bioaccessibility was assessed by subjecting vitamin-enriched emulsions to a standardized in vitro digestion model simulating gastrointestinal conditions. Results showed significantly higher incorporation efficiency in the O/W/O system compared to conventional W/O emulsions, regardless of the physicochemical properties of the vitamins. Both lipophilic (A and D₃) and hydrophilic (folic acid) compounds exhibited a satisfactory retention, highlighting the versatility of the double-emulsion approach. This study represents the first report of simple and multiple oil-continuous emulsions that simultaneously incorporate vitamins A, D₃, and folic acid, providing preliminary evidence of their stability and gastrointestinal release under simulated digestion conditions. Full article

The aim of this study was to evaluate the potential of selected agri-food by-products—apple pomace extract from Malus domestica cv. ‘Grochówka’ and Roman chamomile (Chamaemelum nobile L.) hydrolate—as functional, sustainable ingredients for cosmetic applications. The work focused on their chemical composition, biological activity, formulation performance, and in vivo effects on skin condition. Volatile compounds, phenolic acids, and triterpenoids were analyzed by GC–MS, while total phenolic content, antioxidant capacity, and enzyme inhibitory activity were evaluated in vitro. An oil-in-water emulsion containing the by-products was formulated and, in a 14-day split-face study, assessed for its effects on skin hydration, elasticity, inflammation, sensitivity, pore visibility, and melanin index. Biochemical analyses have shown that chamomile hydrolate is characterized by very low antioxidant activity (DPPH 5.0 ± 1.25%, FRAP 0%) and weak protease inhibition (9.70 ± 1.84%). In contrast, apple extract contained a significant amount of polyphenols (23.94 ± 0.3 mg GAE/g) and showed strong antioxidant properties (DPPH 79.4 ± 2.12%, FRAP 70.56 ± 2.23%; IC₅₀ = 21.5 ± 0.196 mg/mL), which confirms the dominant role of phenolic compounds in its biological activity. This extract also demonstrated significant protease inhibition (60.88 ± 2.35%; IC₅₀ = 15.02 ± 0.47 mg/mL), while its lipase inhibition activity was moderate (10%), which may be beneficial from a cosmetic perspective. The obtained results indicate that apple extract is a valuable raw material with multifaceted biological potential. Overall, the results demonstrate that apple pomace extract and chamomile hydrolate can be effectively valorized as bioactive cosmetic ingredients, supporting both skin health benefits and circular economy principles in sustainable cosmetic formulation. Full article

After implantation in vivo, airway stents are prone to negative biological effects, such as platelet adhesion, aggregation, and blood coagulation, which may lead to vascular occlusion and thrombosis. Therefore, when studying the antithrombotic properties of vascular grafts, it is crucial to construct a fiber film-coated airway stent with antithrombotic properties. In this paper, PTFE/TPU fiber film was prepared by emulsion electrospinning, and heparin aldehyde group was modified to covalently graft with the fiber film to obtain heparin-loaded fiber film (Hep-PT fiber film), and a heparin-loaded PTFE fiber film-coated airway stent (Hep-PT fiber film-coated airway stent) was prepared. Covalent grafting improves the stability of heparin and promotes the long-term stable release of heparin. The loading of heparin increases the fiber nodes between the fiber films, increases the friction between the fibers, and improves the mechanical properties and ability of the fiber film to resist external forces. At the same time, the Hep-PT fiber film-coated airway stent exhibits excellent cytocompatibility, making it an ideal candidate system for airway stent materials. Full article

In this study, a combined approach of molecular dynamics (MD) simulations and experimental bottle tests was employed to systematically investigate the demulsification performance and underlying mechanisms of two distinct demulsifiers—Demulsifier X (SP/BP series and alcohol-initiated polyethers) and Demulsifier Y (AP/AE series and amine-initiated polyethers)—targeting polymer-containing oil-in-water (O/W) emulsions derived from heavy oil polymer flooding. Molecular models for heavy oil, saline water, partially hydrolyzed polyacrylamide (HPAM), and demulsifiers were constructed using BIOVIA Materials Studio software. Their dynamic behaviors at the oil–water interface were simulated within three distinct saline systems containing NaCl, CaCl₂, and MgCl₂, respectively. Simulation results indicated that the demulsifiers effectively displaced interfacial HPAM molecules, increased interfacial tension, and reduced interfacial interaction energy. Experimental bottle tests, evaluating the effects of settling time, temperature, and concentration on dehydration rates and oil content, confirmed that Demulsifier Y outperformed Demulsifier X. Specifically, Demulsifier Y achieved superior dehydration rates with lower dosages, shorter settling times, and reduced temperature requirements under optimal conditions. This work provides both microscopic mechanistic insights and macroscopic experimental validation for the screening and application of high-efficiency demulsifiers. Full article

Increasing attention to environmental performance in construction materials has intensified the need for robust Life Cycle Assessment (LCA) studies on bituminous waterproofing systems. This study addresses the lack of comparative LCAs based on primary data for hot-applied Elastomeric Modified Bitumen (EMB) and cold-applied Bitumen Emulsion (EMBE), two widely used materials with contrasting application methods and environmental profiles. While EMB has been moderately covered in the literature, this study contributes uniquely by providing one of the first LCAs based on primary data for EMBE, a formulation that is increasingly adopted in the construction sector but still underexplored in environmental assessments. The primary industrial data were combined with international LCI datasets (Ecoinvent) to model environmental impacts using SimaPro 9.4.0.3. Results show that EMBE demonstrates better climate performance (611 kg CO₂ eq/t) but is more sensitive to specific additives, especially resins and plasticizers, which significantly increase Ozone Depletion Potential and photochemical ozone formation. The Environmental Product Declaration (EPD) survey analysis further highlights the influence of recycled content, cold mix technologies, and production energy sources on environmental performance. The findings indicate that the selection of waterproofing materials should consider not only technical performance but also the distribution of environmental impacts across the life cycle. Full article

Background and Aims: Injectable lipid emulsions are an integral component of parenteral nutrition, providing energy as well as essential fatty acids. However, conventional soybean oil–based emulsions, which are rich in omega-6 fatty acids, are associated with a risk of exacerbating pro-inflammatory responses and immunosuppression, which is of particular importance in critically ill patients. The aim of this review is to present the significance of the composition of modern injectable lipid emulsions, with particular emphasis on emulsions containing fish oil as a source of omega-3 fatty acids (EPA and DHA), and to discuss their potential clinical benefits in selected critical conditions. Methods: This narrative review discusses the rationale for modern mixed-oil ILE, with a focus on fish oil as a source of EPA and DHA, and summarizes potential clinical benefits in selected critical care settings. Results: Modern injectable lipid emulsions combine long-chain triglycerides derived from soybean oil (omega-6), MCTs, olive oil (omega-9), and fish oil (omega-3). Adjusting the supply of individual fractions affects cell membrane structure, signaling pathways, gene expression, and the profile of lipid mediators produced, including specialized pro-resolving mediators (SPMs). ESPEN guidelines and international recommendations emphasize the need to use lipids in parenteral nutrition, preferring mixed-oil ILE supplemented with fish oil. The cited meta-analyses and clinical studies indicate that omega-3-containing emulsions may reduce the risk of infections and sepsis; shorten hospital stay, ICU length of stay, and duration of mechanical ventilation in patients with sepsis; as well as improve outcomes in acute pancreatitis; lower the risk of delirium; and reduce the incidence of delayed gastric emptying. Conclusions: Available data support the use of mixed-oil ILE supplemented with fish oil in the parenteral nutrition of critically ill patients as a strategy with immunomodulatory and pro-resolving potential that may translate into improved clinical outcomes. However, further well-designed randomized trials are needed to optimize dosing and administration regimens. Full article

As a strategy to valorise onion peel (OP), a phenolic-rich extract was obtained and microencapsulated using the double emulsion technique for improved stability. Both free and microencapsulated OP extracts were added to fresh cheese to enhance its nutritional composition. The extract exhibited a high total phenolic content (TPC) and strong antioxidant capacity towards ABTS and DPPH radicals, with IC₅₀ of 9.5 and 36.1 mg_Extract∙L⁻¹, respectively. The extract demonstrated inhibitory capacities of 71% against α-amylase and 82% towards β-glucosidase. Quercetin was identified as the main phenolic compound, while potassium was the predominant mineral. The microencapsulation yielded an encapsulation efficiency of 91%, with an average particle size of 17.9 µm. Incorporating free and microencapsulated OP extract into the fresh cheese reduced syneresis, a favourable outcome, while preserving moisture levels, protein and ash content, and the pH. The incorporation of the free and microencapsulated OP extract enhanced the TPC and DPPH scavenging capacity of the cheeses. Results demonstrated the potential of using OP extract to enhance the antioxidant properties of fresh cheese, and to reduce syneresis, while promoting sustainability. These outcomes are particularly relevant from an industrial point of view, since an increase in antioxidant content might contribute to extending the product shelf-life. Full article

This study investigated the characteristics and bioactive properties of olive oils obtained from regional Nizip Yaglik (NY) and Kilis Yaglik (KY) olive varieties, encapsulated using maltodextrin (MD) and whey protein isolate (WPI) as wall materials. Olive oils were first emulsified with different WPI–MD ratios (1:1, 1:4, 1:10) and subsequently freeze-dried to produce microcapsule powders. A comprehensive evaluation was conducted, including physicochemical properties (encapsulation efficiency, moisture content, water activity, bulk density, flowability, wettability, particle size, and color), FTIR spectral profiles, morphological features, total phenolic content, and antioxidant activity. The results demonstrated that combining WPI with MD yielded high encapsulation efficiency and favorable reconstitution characteristics, effectively protecting sensitive bioactive constituents from oxidative degradation during processing and storage. Increasing the proportion of MD in the wall matrix improved emulsion stability and microencapsulation yield, while also slightly enhancing powder brightness. FTIR analyses confirmed that the fundamental chemical structure of olive oil was preserved across all formulations. The freeze-dried microcapsules displayed superior stability relative to non-encapsulated oils, retaining higher levels of phenolic compounds and antioxidant capacity. Among the formulations, elevated MD ratios enhanced powder flowability, whereas WPI played a crucial role in emulsification performance and capsule surface integrity. Overall, these findings underscore the effectiveness of MD–WPI blends as promising wall materials for the freeze-drying encapsulation of regional olive oils, offering a viable strategy to preserve their distinctive qualities and bioactive potential for functional food applications. Full article

To enhance the comprehensive performance and interfacial adhesion of conventional emulsified asphalt, an epoxy-functionalized waterborne polyurethane modified emulsified asphalt (EFPU-MEA) was developed using an epoxy-functionalized waterborne polyurethane (EFPU) emulsion and an isocyanate curing agent. Experimental evaluations show that the EFPU-MEA achieves a tensile strength of 1.11 ± 0.05 MPa and an elongation at break of 782.5 ± 45%, demonstrating a well-balanced flexibility and deformation resistance. The interfacial bond between EFPU-MEA and aggregates exhibited robust durability under various stressors, including thermal fluctuations, low-temperature cracking, chemical corrosion, and moisture damage. Quantitative “sandwich” pull-out and shear tests determined the optimal modifier content and spraying quantity to be 15–20% and 1.0 kg/m², respectively. Under these conditions, the system maintained high bond strength following severe freeze–thaw cycles and chemical erosion. Mechanistically, fluorescence microscopy (FM) confirmed a uniform dispersion of EFPU within the asphalt matrix, providing effective physical reinforcement. Furthermore, surface free energy (SFE) analysis and Fourier Transform Infrared (FTIR) spectroscopy revealed that internal chemical crosslinking restructures the binder’s surface thermodynamics, significantly increasing the surface polarity and adhesion work. Finally, road performance tests—including marshall stability, wet track abrasion, and rutting resistance—verified the engineering durability of the EFPU-MEA mixture. These findings provide a theoretical and practical basis for the use of EFPU-MEA in extending the service life of high-grade highway pavements. Full article