Inhibitory Effect of β-Carotene on Helicobacter pylori-Induced TRAF Expression and Hyper-Proliferation in Gastric Epithelial Cells
Department of Food and Nutrition, Brain Korea 21 PLUS Project, College of Human Ecology, Yonsei University, Seoul 03722, Korea
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Author to whom correspondence should be addressed.
Antioxidants 2019, 8(12), 637; https://doi.org/10.3390/antiox8120637
Received: 25 October 2019
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Revised: 29 November 2019
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Accepted: 9 December 2019
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Published: 11 December 2019
(This article belongs to the Special Issue Fighting Radical Species in Human Health: Mitigating Radical Species with Natural and Synthetic Compounds)
Helicobacter pylori infection causes the hyper-proliferation of gastric epithelial cells that leads to the development of gastric cancer. Overexpression of tumor necrosis factor receptor associated factor (TRAF) is shown in gastric cancer cells. The dietary antioxidant β-carotene has been shown to counter hyper-proliferation in H. pylori-infected gastric epithelial cells. The present study was carried out to examine the β-carotene mechanism of action. We first showed that H. pylori infection decreases cellular IκBα levels while increasing cell viability, NADPH oxidase activity, reactive oxygen species production, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activation, and TRAF1 and TRAF2 gene expression, as well as protein–protein interaction in gastric epithelial AGS cells. We then demonstrated that pretreatment of cells with β-carotene significantly attenuates these effects. Our findings support the proposal that β-carotene has anti-cancer activity by reducing NADPH oxidase-mediated production of ROS, NF-κB activation and NF-κB-regulated TRAF1 and TRAF2 gene expression, and hyper-proliferation in AGS cells. We suggest that the consumption of β-carotene-enriched foods could decrease the incidence of H. pylori-associated gastric disorders.
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Keywords:
β-carotene; Helicobacter pylori; hyper-proliferation; NF-κB; tumor necrosis factor receptor-associated factor
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MDPI and ACS Style
Park, Y.; Lee, H.; Lim, J.W.; Kim, H. Inhibitory Effect of β-Carotene on Helicobacter pylori-Induced TRAF Expression and Hyper-Proliferation in Gastric Epithelial Cells. Antioxidants 2019, 8, 637. https://doi.org/10.3390/antiox8120637
AMA Style
Park Y, Lee H, Lim JW, Kim H. Inhibitory Effect of β-Carotene on Helicobacter pylori-Induced TRAF Expression and Hyper-Proliferation in Gastric Epithelial Cells. Antioxidants. 2019; 8(12):637. https://doi.org/10.3390/antiox8120637
Chicago/Turabian StylePark, Yongchae, Hanbit Lee, Joo W. Lim, and Hyeyoung Kim. 2019. "Inhibitory Effect of β-Carotene on Helicobacter pylori-Induced TRAF Expression and Hyper-Proliferation in Gastric Epithelial Cells" Antioxidants 8, no. 12: 637. https://doi.org/10.3390/antiox8120637
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