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Open AccessArticle

Antioxidant Metabolism and Chlorophyll Fluorescence during the Acclimatisation to Ex Vitro Conditions of Micropropagated Stevia rebaudiana Bertoni Plants

1
Group of Fruit Tree Biotechnology, CEBAS-CSIC, 30100 Murcia, Spain
2
Cátedra Emprendimiento en el Ámbito Agroalimentario, Universidad Católica San Antonio de Murcia (UCAM) Campus de los Jerónimos, no. 135 Guadalupe, 30107 Murcia, Spain
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Antioxidants 2019, 8(12), 615; https://doi.org/10.3390/antiox8120615
Received: 19 November 2019 / Revised: 28 November 2019 / Accepted: 30 November 2019 / Published: 3 December 2019
(This article belongs to the Special Issue Antioxidants in Foods)
In this study, the functioning of antioxidant metabolism and photosynthesis efficiency during the acclimatisation of Stevia rebaudiana plants to ex vitro conditions was determined. A high percentage of acclimatised plants (93.3%) was obtained after four weeks. According to the extent of lipid peroxidation, an oxidative stress occurred during the first hours of acclimatisation. A lower activity of monodehydroascorbate reductase (MDHAR) than dehydroascorbate reductase (DHAR) was observed after 2 days of acclimatisation. However, after 7 days of acclimatisation, stevia plants activated the MDHAR route to recycle ascorbate, which is much more efficient energetically than the DHAR route. Superoxide dismutase and catalase activities showed a peak of activity after 7 days of acclimatisation, suggesting a protection against reactive oxygen species. Peroxidase activity increased about 2-fold after 2 days of acclimatisation and remained high until day 14, probably linked to the cell wall stiffening and the lignification processes. In addition, a progressive increase in the photochemical quenching parameters and the electronic transport rate was observed, coupled with a decrease in the non-photochemical quenching parameters, which indicate a progressive photosynthetic efficiency during this process. Taken together, antioxidant enzymes, lipid peroxidation, and chlorophyll fluorescence are proven as suitable tools for the physiological state evaluation of micropropagated plants during acclimatisation to ex vitro conditions.
Keywords: acclimatisation; antioxidant defences; chlorophyll fluorescence; in vitro culture; peroxidase; stevia plants acclimatisation; antioxidant defences; chlorophyll fluorescence; in vitro culture; peroxidase; stevia plants
MDPI and ACS Style

Acosta-Motos, J.R.; Noguera-Vera, L.; Barba-Espín, G.; Piqueras, A.; Hernández, J.A. Antioxidant Metabolism and Chlorophyll Fluorescence during the Acclimatisation to Ex Vitro Conditions of Micropropagated Stevia rebaudiana Bertoni Plants. Antioxidants 2019, 8, 615.

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