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Open AccessArticle

GSTO2 Isoforms Participate in the Oxidative Regulation of the Plasmalemma in Eutherian Spermatozoa during Capacitation

1
Department of Biomedical and Molecular Sciences, Queen’s University, Kingston, ON K7L 3N6, Canada
2
Division of Animal Sciences, College of Food, Agriculture and Natural Resources, Columbia, MO 65211, USA
3
Division of Obstetrics, Gynecology and Women’s Health, School of Medicine, University of Missouri, Columbia, MO 65211, USA
4
Department of Surgery (Urology Division), Faculty of Medicine, McGill University, Montreal, QC H4A 3JI, Canada
*
Author to whom correspondence should be addressed.
Antioxidants 2019, 8(12), 601; https://doi.org/10.3390/antiox8120601
Received: 17 October 2019 / Revised: 26 November 2019 / Accepted: 26 November 2019 / Published: 29 November 2019
(This article belongs to the Special Issue Reactive Oxygen Species and Male Fertility)
In addition to perinuclear theca anchored glutathione-s-transferase omega 2 (GSTO2), whose function is to participate in sperm nuclear decondensation during fertilization (Biol Reprod. 2019, 101:368–376), we herein provide evidence that GSTO2 is acquired on the sperm plasmalemma during epididymal maturation. This novel membrane localization was reinforced by the isolation and identification of biotin-conjugated surface proteins from ejaculated and capacitated boar and mouse spermatozoa, prompting us to hypothesize that GSTO2 has an oxidative/reductive role in regulating sperm function during capacitation. Utilizing an inhibitor specific to the active site of GSTO2 in spermatozoa, inhibition of this enzyme led to a decrease in tyrosine phosphorylation late in the capacitation process, followed by an expected decrease in acrosome exocytosis and motility. These changes were accompanied by an increase in reactive oxygen species (ROS) levels and membrane lipid peroxidation and culminated in a significant decrease in the percentage of oocytes successfully penetrated by sperm during in vitro fertilization. We conclude that GSTO2 participates in the regulation of sperm function during capacitation, most likely through protection against oxidative stress on the sperm surface. View Full-Text
Keywords: glutathione-s-transferase omega 2; capacitation; fertilization; male fertility; oxidative regulation; spermatozoa; reactive oxygen species (ROS) glutathione-s-transferase omega 2; capacitation; fertilization; male fertility; oxidative regulation; spermatozoa; reactive oxygen species (ROS)
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Hamilton, L.E.; Zigo, M.; Mao, J.; Xu, W.; Sutovsky, P.; O’Flaherty, C.; Oko, R. GSTO2 Isoforms Participate in the Oxidative Regulation of the Plasmalemma in Eutherian Spermatozoa during Capacitation. Antioxidants 2019, 8, 601.

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