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Open AccessArticle

Chlorophyll Oxidative Metabolism During the Phototrophic and Heterotrophic Growth of Scenedesmus obliquus

1
Department of Food Science and Technology, Federal University of Santa Maria (UFSM), 97105-900 Santa Maria, Brazil
2
Food Phytochemistry Department, Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), University Campus, Building 46, Carretera de Utrera km. 1, 41013 Sevilla, Spain
*
Author to whom correspondence should be addressed.
Antioxidants 2019, 8(12), 600; https://doi.org/10.3390/antiox8120600
Received: 21 October 2019 / Revised: 26 November 2019 / Accepted: 27 November 2019 / Published: 29 November 2019
(This article belongs to the Special Issue Marine Algal Antioxidants)
Different cultivation strategies have been developed with the aim of increasing the production rate of microalgal pigments. Specifically, biotechnological approaches are designed to increase antioxidant metabolites as chlorophyll and carotenoids. However, although significant advances have been built up, available information regarding both the chlorophyll metabolism and their oxidative reactions in photobioreactors is scarce. To unravel such processes, the detailed chlorophyll and carotenoid fraction of Scenedesmus obliquus has been studied by HPLC-ESI/APCI-hrTOF-MS from phototrophic and heterotrophic cultures. Scenedesmus is provided with a controlled strategy of interconversion between chlorophyll a and b to avoid the formation of reactive oxygen species (ROS) at high irradiances in addition to the photoacclimation of carotenoids. Indeed, precise kinetics of 132-hydroxy- and 151-hydroxy-lactone chlorophyll metabolites shows the existence of a chlorophyll oxidative metabolism as a tool to manage the excess of energy at high light conditions. Unexpectedly, the oxidation under phototrophy favored chlorophyll b metabolites over the chlorophyll a series, while the heterotrophic conditions exclusively induced the formation of 132-hydroxy-chlorophyll a. In parallel, during the first 48 h of growth in the dark, the chlorophyll fraction maintained a promising steady state. Although future studies are required to resolve the biochemical reactions implied in the chlorophyll oxidative metabolism, the present results agree with phytoplankton metabolism. View Full-Text
Keywords: phototrophic; heterotrophic; Scenedesmus; chlorophylls; carotenoids; hydroxy-chlorophyll; oxidative metabolism; ROS; lactone-chlorophyll; photoacclimation phototrophic; heterotrophic; Scenedesmus; chlorophylls; carotenoids; hydroxy-chlorophyll; oxidative metabolism; ROS; lactone-chlorophyll; photoacclimation
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MDPI and ACS Style

Maroneze, M.M.; Zepka, L.Q.; Lopes, E.J.; Pérez-Gálvez, A.; Roca, M. Chlorophyll Oxidative Metabolism During the Phototrophic and Heterotrophic Growth of Scenedesmus obliquus. Antioxidants 2019, 8, 600.

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