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Open AccessArticle

Selenium Treatment Enhanced Clearance of Salmonella in Chicken Macrophages (HD11)

by Zhexi Liu 1,2,†, Jianwei Huang 1,†, Yijuan Nie 1,†, Izhar Hyder Qazi 3,4, Yutao Cao 1,2, Linli Wang 1,2, Yue Ai 1,2, Guangbin Zhou 3,*, Keliang Wu 1,2,* and Hongbing Han 1,2,*
1
Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
2
National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
3
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China
4
Department of Veterinary Anatomy & Histology, Shaheed Benazir Bhutto University of Veterinary and Animal Sciences, Sakrand 67210, Pakistan
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Antioxidants 2019, 8(11), 532; https://doi.org/10.3390/antiox8110532
Received: 11 September 2019 / Revised: 28 October 2019 / Accepted: 1 November 2019 / Published: 7 November 2019
(This article belongs to the Special Issue Selenium and Animal Health)
As an important micronutrient, selenium (Se) plays many essential roles in immune response and protection against pathogens in humans and animals, but underlying mechanisms of Se-based control of salmonella growth within macrophages remain poorly elucidated. In this study, using RNA-seq analyses, we demonstrate that Se treatment (at an appropriate concentration) can modulate the global transcriptome of chicken macrophages HD11. The bioinformatic analyses (KEGG pathway analysis) revealed that the differentially expressed genes (DEGs) were mainly enriched in retinol and glutathione metabolism, revealing that Se may be associated with retinol and glutathione metabolism. Meanwhile, Se treatment increased the number of salmonella invading the HD11 cells, but reduced the number of salmonella within HD11 cells, suggesting that enhanced clearance of salmonella within HD11 cells was potentially modulated by Se treatment. Furthermore, RNA-seq analyses also revealed that nine genes including SIVA1, FAS, and HMOX1 were differentially expressed in HD11 cells infected with salmonella following Se treatment, and GO enrichment analysis showed that these DEGs were mainly enriched in an extrinsic apoptotic signaling pathway. In summary, these results indicate that Se treatment may not only affect retinol and glutathione metabolism in macrophages, but could also inhibit salmonella-induced macrophage apoptosis via an extrinsic apoptotic signaling pathway involving SIVA1. View Full-Text
Keywords: selenium; salmonella; HD11 cells; macrophage; RNA-seq; immune system selenium; salmonella; HD11 cells; macrophage; RNA-seq; immune system
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Liu, Z.; Huang, J.; Nie, Y.; Qazi, I.H.; Cao, Y.; Wang, L.; Ai, Y.; Zhou, G.; Wu, K.; Han, H. Selenium Treatment Enhanced Clearance of Salmonella in Chicken Macrophages (HD11). Antioxidants 2019, 8, 532.

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