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Open AccessArticle

Cell-Type-Specific Modulation of Hydrogen Peroxide Cytotoxicity and 4-Hydroxynonenal Binding to Human Cellular Proteins In Vitro by Antioxidant Aloe vera Extract

1
Department of Biology, Josip Juraj Strossmyer University of Osijek, Cara Hadrijana 8/A, 31000 Osijek, Croatia
2
Faculty of Dental Medicine and Health, Josip Juraj Strossmyer University of Osijek, Cara Hadrijana 10/E, 31000 Osijek, Croatia
3
Laboratory for Oxidative Stress (LabOS), Rudjer Boskovic Institute, Bijenicka 54, 10000 Zagreb, Croatia
4
Faculty of Humanities and Social Sciences, Josip Juraj Strossmyer University of Osijek, L. Jägera 9, 31000 Osijek, Croatia
*
Author to whom correspondence should be addressed.
Antioxidants 2018, 7(10), 125; https://doi.org/10.3390/antiox7100125
Received: 24 July 2018 / Revised: 14 September 2018 / Accepted: 17 September 2018 / Published: 21 September 2018
(This article belongs to the Special Issue Antioxidants and Second Messengers of Free Radicals)
Although Aloe vera contains numerous bioactive components, the activity principles of widely used A. vera extracts are uncertain. Therefore, we analyzed the effects of genuine A. vera aqueous extract (AV) on human cells with respect to the effects of hydrogen peroxide (H2O2) and 4-hydroxynonenal (HNE). Fully developed A. vera leaves were harvested and analyzed for vitamin C, carotenoids, total soluble phenolic content, and antioxidant capacity. Furthermore, human cervical cancer (HeLa), human microvascular endothelial cells (HMEC), human keratinocytes (HaCat), and human osteosarcoma (HOS) cell cultures were treated with AV extract for one hour after treatment with H2O2 or HNE. The cell number and viability were determined using Trypan Blue, and endogenous reactive oxygen species (ROS) production was determined by fluorescence, while intracellular HNE–protein adducts were measured for the first time ever by genuine cell-based HNE–His ELISA. The AV extract expressed strong antioxidant capacities (1.1 mmol of Trolox eq/g fresh weight) and cell-type-specific influence on the cytotoxicity of H2O2, as well as on endogenous production of ROS and HNE–protein adducts induced by HNE treatment, while AV itself did not induce production of ROS or HNE–protein adducts at all. This study, for the first time, revealed the importance of HNE for the activity principles of AV. Since HMEC cells were the most sensitive to AV, the effects of AV on microvascular endothelia could be of particular importance for the activity principles of Aloe vera extracts. View Full-Text
Keywords: Aloe vera; plant extract; antioxidants; cell growth; oxidative stress; reactive oxygen species (ROS); hydrogen peroxide; lipid peroxidation; 4-hydroxynonenal (HNE); cell-based ELISA; HNE–protein adducts; microvascular endothelium Aloe vera; plant extract; antioxidants; cell growth; oxidative stress; reactive oxygen species (ROS); hydrogen peroxide; lipid peroxidation; 4-hydroxynonenal (HNE); cell-based ELISA; HNE–protein adducts; microvascular endothelium
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MDPI and ACS Style

Cesar, V.; Jozić, I.; Begović, L.; Vuković, T.; Mlinarić, S.; Lepeduš, H.; Borović Šunjić, S.; Žarković, N. Cell-Type-Specific Modulation of Hydrogen Peroxide Cytotoxicity and 4-Hydroxynonenal Binding to Human Cellular Proteins In Vitro by Antioxidant Aloe vera Extract. Antioxidants 2018, 7, 125. https://doi.org/10.3390/antiox7100125

AMA Style

Cesar V, Jozić I, Begović L, Vuković T, Mlinarić S, Lepeduš H, Borović Šunjić S, Žarković N. Cell-Type-Specific Modulation of Hydrogen Peroxide Cytotoxicity and 4-Hydroxynonenal Binding to Human Cellular Proteins In Vitro by Antioxidant Aloe vera Extract. Antioxidants. 2018; 7(10):125. https://doi.org/10.3390/antiox7100125

Chicago/Turabian Style

Cesar, Vera; Jozić, Iva; Begović, Lidija; Vuković, Tea; Mlinarić, Selma; Lepeduš, Hrvoje; Borović Šunjić, Suzana; Žarković, Neven. 2018. "Cell-Type-Specific Modulation of Hydrogen Peroxide Cytotoxicity and 4-Hydroxynonenal Binding to Human Cellular Proteins In Vitro by Antioxidant Aloe vera Extract" Antioxidants 7, no. 10: 125. https://doi.org/10.3390/antiox7100125

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