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Antioxidants
Volume 14
Issue 11
10.3390/antiox14111327
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Dietary Methionine Regulates Hepatic Autophagy and Apoptosis via m6A Methylation in Juvenile Megalobrama amblycephala

by
Qianwen Sun
1,2,
Linjie Qian
1,2,
Chuntao Xue
1,2,
Qiushuang Ren
1,2,
Wenqiang Jiang
2,
Yan Lin
2,
Siyue Lu
2,
Zhengyan Gu
2 and
Linghong Miao
1,2,*
1
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China
2
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
*
Author to whom correspondence should be addressed.
Antioxidants 2025, 14(11), 1327; https://doi.org/10.3390/antiox14111327
Submission received: 16 September 2025 / Revised: 23 October 2025 / Accepted: 24 October 2025 / Published: 3 November 2025
(This article belongs to the Special Issue Natural Antioxidants and Aquatic Animal Health—2nd Edition)
Versions Notes

Abstract

This study investigated the epigenetic mechanisms through which graded levels of dietary methionine (Met) regulates growth, muscle quality, and health in juvenile Megalobrama amblycephala fed an all-plant-protein diet. Dietary Met supplementation improved growth performance in a dose-dependent manner and enhanced muscle nutritional quality, particularly protein content and amino acid composition. Optimal Met intake also strengthened hepatic antioxidant defenses, stabilized mitochondrial function, and modulated plasma metabolite profiles, including metabolites associated with antioxidant, anti-inflammatory, and antimicrobial activity. Epigenetic analysis revealed that dietary Met influenced hepatic N6-methyladenosine (m6A) RNA methylation and the expression of genes involved in autophagy and apoptosis, suggesting that these molecular pathways contribute to the observed physiological benefits. Collectively, these findings indicate that appropriate dietary Met of 10.1 g/kg not only supports growth and nutrient utilization but also promotes metabolic and cellular homeostasis through epigenetic regulation. This work provides novel insights into the nutritional and molecular strategies for improving the health and performance of juvenile M. amblycephala under plant-based feeding regimes, with potential implications for sustainable aquaculture practices.
Keywords: Megalobrama amblycephala; all-plant-protein diet; methionine; m6A methylation; wtap/fto Megalobrama amblycephala; all-plant-protein diet; methionine; m6A methylation; wtap/fto

Share and Cite

MDPI and ACS Style

Sun, Q.; Qian, L.; Xue, C.; Ren, Q.; Jiang, W.; Lin, Y.; Lu, S.; Gu, Z.; Miao, L. Dietary Methionine Regulates Hepatic Autophagy and Apoptosis via m6A Methylation in Juvenile Megalobrama amblycephala. Antioxidants 2025, 14, 1327. https://doi.org/10.3390/antiox14111327

AMA Style

Sun Q, Qian L, Xue C, Ren Q, Jiang W, Lin Y, Lu S, Gu Z, Miao L. Dietary Methionine Regulates Hepatic Autophagy and Apoptosis via m6A Methylation in Juvenile Megalobrama amblycephala. Antioxidants. 2025; 14(11):1327. https://doi.org/10.3390/antiox14111327

Chicago/Turabian Style

Sun, Qianwen, Linjie Qian, Chuntao Xue, Qiushuang Ren, Wenqiang Jiang, Yan Lin, Siyue Lu, Zhengyan Gu, and Linghong Miao. 2025. "Dietary Methionine Regulates Hepatic Autophagy and Apoptosis via m6A Methylation in Juvenile Megalobrama amblycephala" Antioxidants 14, no. 11: 1327. https://doi.org/10.3390/antiox14111327

APA Style

Sun, Q., Qian, L., Xue, C., Ren, Q., Jiang, W., Lin, Y., Lu, S., Gu, Z., & Miao, L. (2025). Dietary Methionine Regulates Hepatic Autophagy and Apoptosis via m6A Methylation in Juvenile Megalobrama amblycephala. Antioxidants, 14(11), 1327. https://doi.org/10.3390/antiox14111327

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