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Open AccessArticle

A Mechanism of Gold Nanoparticle Aggregation by Immunoglobulin G Preparation

1
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky prospect 33, 119071 Moscow, Russia
2
Central Tuberculosis Research Institute, Yauz alley 2, 107564 Moscow, Russia
*
Author to whom correspondence should be addressed.
Appl. Sci. 2020, 10(2), 475; https://doi.org/10.3390/app10020475
Received: 26 November 2019 / Revised: 7 January 2020 / Accepted: 7 January 2020 / Published: 9 January 2020
(This article belongs to the Special Issue Nanoparticles in Sensing and Labelling)
Conjugates of gold nanoparticles (GNPs) and antibodies are widely used in various fields of biochemistry and microbiology. However, the procedure for obtaining such conjugates remains precarious, and the properties of conjugates differ significantly for different antibody clones. One of the most common problems is the aggregation of GNPs in the course of their conjugation with antibodies. This article considers an example of the conjugation of monoclonal antibodies with non-stable aggregating product. The composition of the antibody preparation was studied using electrophoresis, asymmetrical flow field-flow fractionation, and ultracentrifugation. It was shown that the component that causes the aggregation of the GNPs is the light chains of immunoglobulins that appear due to the spontaneous decay of the antibodies. After separation of the fraction with a molecular weight of less than 30 kDa, stable conjugates of antibodies with GNPs were obtained. The high functional activity of the obtained conjugates was confirmed by immunochromatography. View Full-Text
Keywords: gold nanoparticles; conjugation; immobilization; flocculation; fragments of antibodies gold nanoparticles; conjugation; immobilization; flocculation; fragments of antibodies
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Sotnikov, D.V.; Safenkova, I.V.; Zherdev, A.V.; Avdienko, V.G.; Kozlova, I.V.; Babayan, S.S.; Gergert, V.Y.; Dzantiev, B.B. A Mechanism of Gold Nanoparticle Aggregation by Immunoglobulin G Preparation. Appl. Sci. 2020, 10, 475.

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