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Microorganisms
Volume 8
Issue 12
10.3390/microorganisms8122052
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Background:
Communication

Molecular Characterization of Staphylococcus aureus Isolated from Chronic Infected Wounds in Rural Ghana

by
Manuel Wolters
1,
Hagen Frickmann
2,3,
Martin Christner
1,
Anna Both
1,
Holger Rohde
1,
Kwabena Oppong
4,
Charity Wiafe Akenten
4,
Jürgen May
5,6 and
Denise Dekker
6,7,*
1
Institute of Medical Microbiology, Virology and Hygiene, Universitiy Medical Center Hamburg-Eppendorf (UKE), 20251 Hamburg, Germany
2
Department of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, 20359 Hamburg, Germany
3
Institute for Medical Microbiology, Virology and Hygiene, University Medicine Rostock, 18057 Rostock, Germany
4
Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kumasi, Ghana
5
Tropical Medicine II, Universitiy Medical Center Hamburg-Eppendorf (UKE), 20251 Hamburg, Germany
6
Department of Infectious Disease Epidemiology, Bernhard Nocht Institute for Tropical Medicine (BNITM), 20359 Hamburg, Germany
7
German Centre for Infection Research (DZIF), Hamburg-Lübeck-Borstel-Riems, 38124 Braunschweig, Germany
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(12), 2052; https://doi.org/10.3390/microorganisms8122052
Submission received: 2 December 2020 / Revised: 17 December 2020 / Accepted: 17 December 2020 / Published: 21 December 2020
(This article belongs to the Special Issue Staphylococcal Infections (Host and Pathogenic Factors))
Review Reports Versions Notes

Abstract

Background: Globally, Staphylococcus aureus is an important bacterial pathogen causing a wide range of community and hospital acquired infections. In Ghana, resistance of S. aureus to locally available antibiotics is increasing but the molecular basis of resistance and the population structure of S. aureus in particular in chronic wounds are poorly described. However, this information is essential to understand the underlying mechanisms of resistance and spread of resistant clones. We therefore subjected 28 S. aureus isolates from chronic infected wounds in a rural area of Ghana to whole genome sequencing. Results: Overall, resistance of S. aureus to locally available antibiotics was high and 29% were Methicillin resistant Staphylococcus aureus (MRSA). The most abundant sequence type was ST88 (29%, 8/28) followed by ST152 (18%, 5/28). All ST88 carried the mecA gene, which was associated with this sequence type only. Chloramphenicol resistance gene fexB was exclusively associated with the methicillin-resistant ST88 strains. Panton-Valentine leukocidin (PVL) carriage was associated with ST121 and ST152. Other detected mechanisms of resistance included dfrG, conferring resistance to trimethoprim. Conclusions: This study provides valuable information for understanding the population structure and resistance mechanisms of S. aureus isolated from chronic wound infections in rural Ghana.
Keywords: rural Ghana; molecular epidemiology; chronic wounds; Staphylococcus aureus rural Ghana; molecular epidemiology; chronic wounds; Staphylococcus aureus

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MDPI and ACS Style

Wolters, M.; Frickmann, H.; Christner, M.; Both, A.; Rohde, H.; Oppong, K.; Akenten, C.W.; May, J.; Dekker, D. Molecular Characterization of Staphylococcus aureus Isolated from Chronic Infected Wounds in Rural Ghana. Microorganisms 2020, 8, 2052. https://doi.org/10.3390/microorganisms8122052

AMA Style

Wolters M, Frickmann H, Christner M, Both A, Rohde H, Oppong K, Akenten CW, May J, Dekker D. Molecular Characterization of Staphylococcus aureus Isolated from Chronic Infected Wounds in Rural Ghana. Microorganisms. 2020; 8(12):2052. https://doi.org/10.3390/microorganisms8122052

Chicago/Turabian Style

Wolters, Manuel, Hagen Frickmann, Martin Christner, Anna Both, Holger Rohde, Kwabena Oppong, Charity Wiafe Akenten, Jürgen May, and Denise Dekker. 2020. "Molecular Characterization of Staphylococcus aureus Isolated from Chronic Infected Wounds in Rural Ghana" Microorganisms 8, no. 12: 2052. https://doi.org/10.3390/microorganisms8122052

APA Style

Wolters, M., Frickmann, H., Christner, M., Both, A., Rohde, H., Oppong, K., Akenten, C. W., May, J., & Dekker, D. (2020). Molecular Characterization of Staphylococcus aureus Isolated from Chronic Infected Wounds in Rural Ghana. Microorganisms, 8(12), 2052. https://doi.org/10.3390/microorganisms8122052

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