Probiotic Effects of a Novel Strain, Acinetobacter KU011TH, on the Growth Performance, Immune Responses, and Resistance against Aeromonas hydrophila of Bighead Catfish (Clarias macrocephalus Günther, 1864)

Bunnoy, Anurak; Na-Nakorn, Uthairat; Srisapoome, Prapansak

doi:10.3390/microorganisms7120613

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Probiotic Effects of a Novel Strain, Acinetobacter KU011TH, on the Growth Performance, Immune Responses, and Resistance against Aeromonas hydrophila of Bighead Catfish (Clarias macrocephalus Günther, 1864)

Anurak Bunnoy

Uthairat Na-Nakorn

² and

Prapansak Srisapoome

^1,*

Laboratory of Aquatic Animal Health Management, Department of Aquaculture, Faculty of Fisheries, Kasetsart University, 50 Paholayothin Rd, Ladyao, Chatuchak, Bangkok 10900, Thailand

Laboratory of Aquatic Animal Genetics, Department of Aquaculture, Faculty of Fisheries, Kasetsart University, 50 Paholayothin Rd, Ladyao, Chatuchak, Bangkok 10900, Thailand

Author to whom correspondence should be addressed.

Microorganisms 2019, 7(12), 613; https://doi.org/10.3390/microorganisms7120613

Received: 19 September 2019 / Revised: 11 October 2019 / Accepted: 23 November 2019 / Published: 25 November 2019

(This article belongs to the Special Issue Strengthening Mucosal Immunity and the Intestinal Microbiome with Probiotics and Prebiotics)

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Abstract

In the present study, the novel probiotic strain Acinetobacter KU011TH with an evident lack of pathogenicity in catfish was experimented. Three practical administration routes, namely, feed additive (FD), water-soluble additive (SOL), and a combination route (FD+SOL), were applied in two sizes of catfish. After 120 days of FD+SOL administration, catfish fingerlings (15 g) exhibited a significant improvement in all tested growth performance parameters. For 15- and 30-day applications at the juvenile stage (150 g), phagocytic activity, phagocytic index, lysozyme activity, respiratory burst activity, alternative complement pathway, and bactericidal activity were significantly increased. Furthermore, probiotic-administered bighead catfish exhibited an upregulated expression of several immune-related genes in tested organs. Significant colonization by Acinetobacter KU011TH in rearing water and on skin and gills was observed among experimental groups. Histological analysis clearly indicated enhanced physical characteristics of skin mucosal immunity in the treated groups. No histopathological changes in the gills, skin, intestine or liver were observed among the fish groups. Interestingly, after challenge with Aeromonas hydrophila, the survival rates of the treated groups were significantly higher than those of the controls. In conclusion, the novel probiont Acinetobacter KU011TH provides a potent strategy for improvement in growth and disease resistance, which is an important steppingstone for sustaining catfish aquaculture. View Full-Text

Keywords: Acinetobacter KU011TH; Clarias macrocephalus; probiotics; growth performance; innate immune responses; skin physical barriers; immune-related gene; Aeromonas hydrophila

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Bunnoy, A.; Na-Nakorn, U.; Srisapoome, P. Probiotic Effects of a Novel Strain, Acinetobacter KU011TH, on the Growth Performance, Immune Responses, and Resistance against Aeromonas hydrophila of Bighead Catfish (Clarias macrocephalus Günther, 1864). Microorganisms 2019, 7, 613. https://doi.org/10.3390/microorganisms7120613

AMA Style

Bunnoy A, Na-Nakorn U, Srisapoome P. Probiotic Effects of a Novel Strain, Acinetobacter KU011TH, on the Growth Performance, Immune Responses, and Resistance against Aeromonas hydrophila of Bighead Catfish (Clarias macrocephalus Günther, 1864). Microorganisms. 2019; 7(12):613. https://doi.org/10.3390/microorganisms7120613

Chicago/Turabian Style

Bunnoy, Anurak, Uthairat Na-Nakorn, and Prapansak Srisapoome. 2019. "Probiotic Effects of a Novel Strain, Acinetobacter KU011TH, on the Growth Performance, Immune Responses, and Resistance against Aeromonas hydrophila of Bighead Catfish (Clarias macrocephalus Günther, 1864)" Microorganisms 7, no. 12: 613. https://doi.org/10.3390/microorganisms7120613

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Probiotic Effects of a Novel Strain, Acinetobacter KU011TH, on the Growth Performance, Immune Responses, and Resistance against Aeromonas hydrophila of Bighead Catfish (Clarias macrocephalus Günther, 1864)

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