In the original publication [1], there was a mistake in Table 1 and Table 7 as published. The corrected tables appear below.
In Table 1 for the 16S rRNA partial amplification, a different combination of the cited primers was used. Regarding the dnaK primer sequences, a formatting oversight resulted in identical representations for forward and reverse primers.
Table 1.
Target genes, primers, thermal conditions, and reagent protocol used in the PCR assays for hemoplasmas based on the 16S rRNA, 23S rRNA, RNAse P, and dnaK genes.
Table 1.
Target genes, primers, thermal conditions, and reagent protocol used in the PCR assays for hemoplasmas based on the 16S rRNA, 23S rRNA, RNAse P, and dnaK genes.
| Target Gene | Primer Sequences | Thermal Conditions | Reagent Volumes and Concentrations | Fragment Size | Primers Reference |
|---|---|---|---|---|---|
| 16S rRNA | 1st round: 5′-AGAGTTTGATCCTGGCTCAG-3’ 5′-TACCTTGTTACGACTTAACT-3′ 2nd round: 5′-ATATTCCTACGGGAAGCAGC-3′ 5′-TACCTTGTTACGACTTAACT-3′ | 95 °C for 5 min, followed by 35 cycles of denaturation at 95 °C for 30 s, annealing at 57 °C for 30 s, extension at 72 °C for 1 min, and final extension at 72 °C for 10 min for both rounds. | 1st reaction: 2.5 μL from 10X Buffer, 0.75 μL from 50 mM MgCl2, 2 μL from 10 mM dNTP mix, 1 μL from each primer at 10 mM, 0.25 μL from 5 U/μL Taq polymerase, 12.5 μL from ultrapurified water and 5 μL from template DNA. 2nd reaction: Ultrapurified water (16.5 μL) and template DNA (1 μL) quantity changes. | ~1107 bp | Harasawa et al., 2014; Di Cataldo et al., 2020 |
| 23S rRNA | 5′-TGAGGGAAAGAGCCCAGAC-3′ 5′-GGACAGAATTTACCTGACAAGG-3′ | 94 °C for 3 min, followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at 54 °C for 30 s, extension at 72 °C for 1 min, and final extension at 72 °C for 10 min. | 2.5 μL from 10X Buffer, 0.75 μL from 50 mM MgCl2, 2 μL from 10 mM dNTP mix, 1 μL from each primer at 10 mM, 0.25 μL from 5 U/μL Taq polymerase, 12.5 μL from ultrapurified water and 5 μL from template DNA. | ~800 bp | Mongruel et al., 2020 |
| RNAseP | 5′-GATKGTGYGAGYATATAA AAAATAAARCTCRAC-3′ 5′-GMGGRGTTTACCGCGTTTCAC-3′ | 95 °C for 2 min, followed by 50 cycles of denaturation at 94 °C for 30 s, annealing at 59 °C for 30 s, extension at 72 °C for 30 s and final extension at 72 °C for 1 min. | 2.5 μL from 10X Buffer, 1.0 μL from 50 mM MgCl2, 2 μL from 10 mM dNTP mix, 1 μL from each primer at 10 mM, 0.25 μL from 5 U/μL Taq polymerase, 12.25 μL from ultrapurified water and 5 μL from template DNA. | ~164 bp | Maggi et al., 2013 |
| dnaK | 5′-GGGTGGAGATGATTGAGACCA-3’ 5′-AGCCACCCCTCCTAGAGTTT-3' | 95 °C for 5 min, followed by 45 cycles of denaturation at 95 °C for 20 s, annealing at 55.5 °C for 30 s, extension at 72 °C for 45 s and final extension at 72 °C for 7 min. | 2.25 μL from 10X Buffer, 1.0 μL from 50 mM MgCl2, 2 μL from 10 mM dNTP mix, 1 μL from each primer at 10 mM, 0.15 μL from 5 U/μL Taq polymerase, 12.6 μL from ultrapurified water and 5 μL from template DNA. | ~544 bp | Descloux et al., 2020 |
In Table 7 the statistical analysis was calculated based on the total sampled animals. However, DNA samples from three animals failed to amplify both of the tested housekeeping genes and were consequently excluded from hemoplasma molecular screening, as explained in the Materials and Methods and Results sections. Although the significance of the tested variables did not change, the statistical analysis was corrected. The corrected table appears below.
Table 7.
Statistical analysis comparing the occurrence of hemotropic Mycoplasma sp. in sampled tapirs and outcomes (gender, sampling location, and age).
Table 7.
Statistical analysis comparing the occurrence of hemotropic Mycoplasma sp. in sampled tapirs and outcomes (gender, sampling location, and age).
| 16S rRNA Mycoplasma spp. PCR | ||||||
|---|---|---|---|---|---|---|
| Variable | +/n | (%) | OR | 95% CI | p-Value | |
| Gender | Male Female Total | 21/50 15/49 36/99 | 42 30.61 | 1.641 | 0.71–3.75 | 0.1198 |
| Location | Pantanal Cerrado Total | 30/61 6/38 36/99 | 49.18 15.79 | 5.161 | 1.887–14.11 | 0.0003915 |
| Age | Sub-adult Adult Total | 20/46 16/53 36/99 | 43.48 30.19 | 1.779 | 0.77–4.06 | 0.08528 |
+, Number of positive animals; n, number of samples; 95% CI, 95% confidence interval; OR, odds ratio. p-values < 0.05 were considered statically significant and are highlighted in bold.
The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.
Reference
- Mongruel, A.C.B.; Medici, E.P.; Canena, A.d.C.; Calchi, A.C.; Machado, R.Z.; André, M.R. Expanding the Universe of Hemoplasmas: Multi-Locus Sequencing Reveals Putative Novel Hemoplasmas in Lowland Tapirs (Tapirus terrestris), the Largest Land Mammals in Brazil. Microorganisms 2022, 10, 614. [Google Scholar] [CrossRef] [PubMed]
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. |
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).