Molecular Diagnostics and Pathogenesis of Fungal Pathogens on Bast Fiber Crops.

Bast fibers and products derived from them are undergoing a resurgence in demand in the global market. However, fungal diseases have become an important factor limiting their yield and quality, causing devastating consequences for the production of bast fiber crops in many parts of the world. Thus, there is a high demand for effective control and prevention strategies against fungal pathogens. Having rapid, specific, sensitive, and cost-effective tests that can be used for early and accurate diagnosis of disease agents is an essential step of such strategies. The objective of this study was to review the current status of research on molecular diagnosis of fungal pathogens on bast fiber crops. Our search of PubMed identified nearly 20 genera of fungal pathogens on bast fiber crops, among which the five most common genera were Colletotrichum, Pythium, Verticillium, Fusarium, and Golovinomyces. The gene regions that have been used for molecular identifications of these fungi include internal transcribed spacer (ITS), translation elongation factor 1-α (EF-1α), ß-tubulin, calmodulin (CAL), histone subunit 3 (H3), glyceraldehydes-3-phosphate dehydrogenase (GAPDH), etc. We summarize the molecular assays that have been used to identify these fungi and discuss potential areas of future development for fast, specific, and accurate diagnosis of fungal pathogens on bast fiber crops.


Introduction
Plant infectious diseases are among the most important constraints on the quality and yield of crops. It is estimated that plant diseases cause losses of 10%-15% of the world's major crops, with direct economic losses of up to hundreds of billions of dollars each year. About 70%-80% of crop diseases are caused by fungal pathogens and the damage can be very serious, significantly reducing the yield and quality of many staple food crops and economic crops like fruits, vegetables, and fiber crops [1]. In addition, several fungal pathogens can secrete a variety of toxins and metabolites harmful to humans and animals, posing a great threat to the safety of agricultural products [2]. At present, most control measures against plant fungal pathogens rely on the applications of broad-spectrum fungicides. However, such fungicides not only increase production costs, but also can bring problems such as environmental pollution, fungicide resistance, and persistent residues on foods and other consumer goods with further implications for human health. In order to minimize the damage to crops caused by fungal diseases, as well as to maximize productivity and ensure agricultural sustainability, early detection and quantification of fungal pathogens is essential for disease prevention

Bast Fiber Crops
Bast fiber crops are an important group of economic crops for the purpose of harvesting fibers from stems [20]. These fibers are sclerenchyma fibers associated with the phloem of plants. They arise either with primary tissues from the apical meristem, or with secondary tissues produced by the lateral meristem. Bast fiber is one of four major types of natural plant fibers, with the other three being leaf fiber (e.g., banana and pineapple fibers), fruit and seed fiber (e.g., cotton and coconut fiber), and stalk fiber (e.g., straw fiber from rice, wheat, and bamboo). Bast fiber crops comprise six main species (flax, hemp, ramie, kenaf, jute, and sunn hemp) that are broadly cultivated (Table 1) as well as a few others (kudzu, linden, milkweed, nettle, okra, and paper mulberry) with more limited fiber production [21]. Table 1 summarizes the main bast fiber crops, including their geographic distributions, habitats, commercial use, and main fungal diseases. Most bast fiber crops have good fiber strength and are often used to make ropes, twine, packaging materials, and industrial thick cloth [22]. Although the commercial importance of bast fibers has been challenged by the rapid growth of other natural fibers (including animal fibers) and chemical fibers from petroleum, there have been renewed interests in bast fibers in recent years. The renewed interests are driven by several factors, including the bast fibers being a renewable resource for producing high-strength and lightweight composite materials for the textile, construction, and automobile industries. In addition, high contents of crude protein, unsaturated fatty acid, and functional compounds that are beneficial for human health have been found in the seeds, flowers, and leaves of these crops. As a result, these crops have been the source of materials for making a diversity of functional feeds for animals, as well as foods, food additives, and therapeutic drugs for humans [22]. An example of functional compounds is cannabinoids in industrial hemp that are attracting broad attention from both healthcare professionals and the general public.
The increasing medical interests and commercial demands for bast fiber crops have resulted in expanding areas for growing bast fiber crops and changing cultivation practices. For example, it is now common to use the same piece of land continuously to grow the same bast fiber crop. However, in such a situation, certain disease agents will likely be enriched in the crop fields, causing increasingly severe diseases to the crops. In the last twenty years, dozens of fungal diseases in bast fiber crops have been identified. The loss of productivity due to fungal diseases was estimated at 10%-50%, with variations attributed to crop type, geographic region, size of planting area, and growth cycle of the crop (especially for continuously growing fields) [22]. In addition, fungal infections also damage the quality of bast fiber and reduce their commercial value. Therefore, having a stable and predictive crop production is crucial for the healthy development of the whole industry. Furthermore, having an early and accurate diagnosis of fungal pathogens infecting bast fiber crops would contribute to disease surveillance and to the implementation of a rational disease management strategy for these crops. Based on the findings retrieved from PubMed, below we review the main fungal disease agents of bast fiber crops, and the principal molecular markers and assays that have been used for detecting fungal pathogens of these crops.

Fungal Pathogens of Bast Fiber Crops
As shown in Table 1, most bast fiber crops can grow in a diversity of geographic regions and ecological niches. However, some of them have relatively limited geographic and/or ecological distributions and can't grow well in certain environments. As a result, the types of land used to cultivate certain bast fiber crops may be limited and the same fields may be used to grow the same crop over many years. Even for bast fiber crops with broad ecological adaptability, the limited agricultural land in certain regions and the drive to seek high commercial benefits often mean that only certain types of fields are used for growing each specific crop. In these fields, fungal infectious diseases often increase over time, leading to large yield loss, or even total destruction of the harvest. Fungal pathogens occurring on bast fiber crops are taxonomically very broad ( Table 2). Below we describe the major genera and species of fungal pathogens impacting bast fiber crops.
Fungi from the ascomycetous genus Colletotrichum cause anthracnose disease in a wide range of plant species, often resulting in significant economic losses [23]. The following six Colletotrichum species have been reported from bast fiber crops: Colletotrichum phormii, Colletotrichum fructicola, Colletotrichum siamense, Colletotrichum corchorumcapsularis, Colletotrichum higginsianum, and Colletotrichum gloeosporioides. These species have been reported from flax, jute, kenaf, and ramie, causing an average crop loss of about 20%, with certain crop losses up to 50%. The anthracnose diseases on bast fiber crops have been reported from the US, Australia, and China (Table 2). Symptoms on these bast fiber crops include dark brown and often fusiform to ellipsoidal or irregularly shaped spots on leaves, petioles, and stems. New leaves and shoots are among the most susceptible to anthracnose infections [24][25][26][27][28][29][30].
Pathogens from the oomycete genus Pythium cause crown rot and root rot in both ramie and hemp crops as well as in marijuana plants grown in both field and hydroponic conditions [31,32]. Five species from this genus are pathogenic against ramie and hemp plants: Pythium vexans, Pythium dissotocum, Pythium myriotylum, Pythium aphanidermatum, and Pythium ultimum. Both crown and root rots are more common in cool conditions than in hot conditions, particularly in low lying or flood-prone areas where hemp is intensively grown. Findings from field tests indicate P. aphanidermatum could infect different tissues and organs of cannabis plants, resulting in rot, wilt, and eventual collapse of the whole host plant [31][32][33][34][35][36].
Fungi from the genus Verticillium are also persistent pathogens affecting the xylem vessels of susceptible plants. Verticillium fungi can survive for a long time in the soil [37]. At present, three Verticillium species are known to be associated with flax: Verticillium dahliae, Verticillium tricorpus, and Verticillium longisporum. Among these three, V. dahliae is the only confirmed pathogen of flax crop. This pathogen can cause flax wilt and lead to non-negligible yield losses and depreciated fibers, both of which are difficult to deal with after harvesting. Verticillium wilt caused by V. dahliae often triggers wilt and necrosis in the leaves, brown discoloration of epidermis, and vascular tissues in the main root and stem. V. dahliae can form microsclerotia that are resistant to a variety of stresses and be easily dispersed to other fields by hiding in host debris [36][37][38]. Two other Verticillium species, named V. tricorpus and V. longisporum, have been found in the flax culture soil, but their pathogenicity to flax remains to be determined [38].
Wilting and crown rot diseases, caused by fungi from the genus Fusarium and often accompanied by vascular and pith discoloration symptoms, are among the most devastating diseases in bast fiber crops. Fusarium pathogens of bast fiber crops are present mainly in the F. oxysporum species complex (FOSC) [39][40][41]. The reported diseases on bast fiber crops associated with FOSC include jute brown wilt, hemp wilt, and crown rot. Several other Fusarium species, such as Fusarium solani, Fusarium brachygibbosum, and Fusarium udum f. sp. crotalariae can also cause wilt and crown rot in hemp, jute, and sunn hemp. Fusarium spp. are common residents in agricultural soils and can live a saprophytic lifestyle. However, most Fusarium species can cause diseases in a diversity of plants, including bast fiber crops [39][40][41]. At present, the genetic basis for the broad host range of Fusarium species is Pathogens 2020, 9, 223 5 of 19 largely unknown. However, their broad host ranges and ability to grow and survive in a diversity of environments makes it very challenging to prevent and control these pathogens.
In recent years, powdery mildew on hemp and sunn hemp, caused by fungal pathogens from the genus Golovinomyces, were observed on indoor or field-grown plants in multiple locations in North America. The species from this genus that cause diseases in bast fiber crops include Golovinomyces cichoracearum, the agent of sunn hemp powdery mildew, and Golovinomyces spadiceus and Golovinomyces cichoracearum sensu lato, which are responsible for powdery mildew on hemp. Similarly, Podosphaera macularis and Leveillula taurica can also cause hemp powdery mildew [42][43][44]. Hemp powdery mildew ranges in incidence from 20% to 35% on several varieties. The disease symptoms first appear as inconspicuous white patches on leaves and stems. As the disease progresses, colonies like mycelia, conidiophores, and conidia may expand and spread to flower bracts and buds, including those of other plants. The disease spreads readily to asymptomatic hosts [42][43][44][45].
Fungi from the genus Alternaria have a relatively limited host range among bast fiber crops. Alternaria alternata can cause leaf spot diseases in ramie and cannabis plants [46,47]. The disease symptoms often appear as small brown or circular spots in the leaves on cannabis plants, or as irregular and necrotic lesions on ramie leaves. The disease incidence ranges from 11.8%-30% in southern China. Productivity-wise, black leaf spot in ramie, caused by A. alternata, may reduce the yields of leaves and shoots by 20%-50% [46,47].

Development of Molecular Identification of Bast Fiber Fungal Pathogens
At present, most diagnosis of bast fiber diseases rely on disease symptoms and, when available, cultural characteristics of isolated fungal pathogens on artificial media. However, it is often difficult to identify the underlying pathogen based on those characters alone. For example, the disease symptoms of Verticillium wilt in hemp is very similar to Fusarium wilt and the pathogen species in both genera can invade a wide range of economical crops [37][38][39]. In addition, it is difficult to distinguish the species within most fungal genera based on morphological features alone. However, most of them are relatively easy to identify using molecular markers, as described below (Table 2; Table 3).
As early as 1997, a PCR-based method was used to help identify fungal pathogens of bast fiber crops. Specifically, McPartland et al. [52] amplified part of the 28S ribosomal RNA (rRNA) gene followed by EcoR I/Hind III digestion and electrophoresis to differentiate hemp fungal pathogens, and named two new species: Micropeltopsis cannabis sp. nov. and Orbilia luteola (Roum.) comb. nov. However, there were relatively few reports of fungal pathogens on bast fiber crops between 1998 and 2009, likely due to limited production of bast fiber crops and an emphasis on chemical fiber and other natural fibers. During this period, the acreage and production of bast fiber crops were low and there was limited research on these crops. Since 2009, with increasing production and research on bast fiber crops, there have been increasing reports on infectious diseases, including fungal diseases, on these crops [23]. This is especially true over the last five years when a large number of fungal pathogens were reported from bast fiber crops and many of these were identified based on molecular markers (Figure 1). Pathogens 2020, 9, x FOR PEER REVIEW 10 of 21

Development of Molecular Identification of Bast Fiber Fungal Pathogens
At present, most diagnosis of bast fiber diseases rely on disease symptoms and, when available, cultural characteristics of isolated fungal pathogens on artificial media. However, it is often difficult to identify the underlying pathogen based on those characters alone. For example, the disease symptoms of Verticillium wilt in hemp is very similar to Fusarium wilt and the pathogen species in both genera can invade a wide range of economical crops [37][38][39]. In addition, it is difficult to distinguish the species within most fungal genera based on morphological features alone. However, most of them are relatively easy to identify using molecular markers, as described below ( Table 2;  Table 3).
As early as 1997, a PCR-based method was used to help identify fungal pathogens of bast fiber crops. Specifically, McPartland et al. [52] amplified part of the 28S ribosomal RNA (rRNA) gene followed by EcoR I/Hind III digestion and electrophoresis to differentiate hemp fungal pathogens, and named two new species: Micropeltopsis cannabis sp. nov. and Orbilia luteola (Roum.) comb. nov. However, there were relatively few reports of fungal pathogens on bast fiber crops between 1998 and 2009, likely due to limited production of bast fiber crops and an emphasis on chemical fiber and other natural fibers. During this period, the acreage and production of bast fiber crops were low and there was limited research on these crops. Since 2009, with increasing production and research on bast fiber crops, there have been increasing reports on infectious diseases, including fungal diseases, on these crops [23]. This is especially true over the last five years when a large number of fungal pathogens were reported from bast fiber crops and many of these were identified based on molecular markers ( Figure 1).  Table 2. Details of primers are shown in Table 3.
According to the National Center for Biotechnology Information (NCBI) PubMed, the most common literature on the molecular identification of fungal pathogens on bast fiber crops has been on hemp (including both industrial hemp and medicinal marijuana), accounting for ~45% of all published articles. This was then followed by flax and kenaf (at ~14% each), ramie (11%), and the rest being jute and sunn hemp. However, most of these reports were case reports. Below we present a summary review on this topic.

Target DNA Selection and Molecular Assays of Fungal Pathogens on Bast Fiber Crops
Over the last three decades, several types of DNA-based methods have been developed and widely used to detect plant fungal pathogens. The invention of PCR technology using a thermostable polymerase by Kary Mullis gave birth to PCR in the early 1980s [4]. The invention of PCR has led to a diversity of PCR-based methods for fungal pathogen detections based on variations in DNA sequences within and among species (Figure 1, Table 2). Among these methods, qPCR is probably the most common molecular technology and it can be used for quantitative measurement of RNA  Table 2. Details of primers are shown in Table 3.
According to the National Center for Biotechnology Information (NCBI) PubMed, the most common literature on the molecular identification of fungal pathogens on bast fiber crops has been on hemp (including both industrial hemp and medicinal marijuana), accounting for~45% of all published articles. This was then followed by flax and kenaf (at~14% each), ramie (11%), and the rest being jute and sunn hemp. However, most of these reports were case reports. Below we present a summary review on this topic.

Target DNA Selection and Molecular Assays of Fungal Pathogens on Bast Fiber Crops
Over the last three decades, several types of DNA-based methods have been developed and widely used to detect plant fungal pathogens. The invention of PCR technology using a thermostable polymerase by Kary Mullis gave birth to PCR in the early 1980s [4]. The invention of PCR has led to a diversity of PCR-based methods for fungal pathogen detections based on variations in DNA sequences within and among species (Figure 1, Table 2). Among these methods, qPCR is probably the most common molecular technology and it can be used for quantitative measurement of RNA and DNA, targeting both single nucleotide polymorphisms (SNPs) and copy number variations. qPCR allows not only the detection of whether a specific pathogen(s) is present in the sample, but also the quantification of pathogen levels in host tissues [5,6]. To improve the efficiency of conventional PCR, other methods have been coupled with PCR for plant fungal pathogen detection. For example, PCR in combination with enzyme-linked immunosorbent assay (ELISA) has been successfully applied to detect fungi, viruses, and bacteria, with high specificity [56]. Similarly, the highly specific IC-PCR approach can increase the sensitivity by 250 folds compared to conventional PCR amplification [7,8]. For absolute quantification without the need for references and standard curves, dd-PCR is the method of choice-this method is based on the combined technology of water-oil emulsion droplet and PCR [9]. In field conditions without ready access to laboratory equipment, LAMP can provide fast identifications of samples. LAMP uses six primers that are highly specific to target sites in a specific gene [10]. It can be carried out at a constant temperature in a short reaction time (<30 min). It is sensitive and cost-effective, potentially making it an ideal method for field detection of plant pathogens [57].
As shown in Table 2, PCR-based methods have been used as the main approach for detecting fungal pathogens in bast fiber crops. This pattern is similar to the detections of fungal pathogens in other crops in general. A number of DNA fragments and genes have been explored as potential targets for PCR-based detections, including the ribosomal RNA gene cluster, conserved housekeeping genes, and genes involved in the production of secondary metabolites, including mycotoxins [58][59][60]. Table 3 summarizes the genes and their primers that have been used for the detection and diagnostics of fungal pathogens on bast fiber crops. We would like to note that the molecular analyses reported so far for identifying fungal pathogens on bast fiber crops have been primarily using pure fungal strains, not those from diseased plant tissues. There is a large gap in applying these molecular methods in field conditions as a point-of-care test.
Among the DNA fragments that have been used for fungal pathogen detection, the most frequently used is the ribosomal RNA gene cluster. This gene cluster is composed of up to hundreds of repeating units with each unit containing the genes encoding the small (18S) ribosomal RNA subunit, the internal transcribed spacer (ITS) regions 1 and 2 that are separated by the 5.8S rRNA subunit, and the large (28S) ribosomal RNA subunit, with the intergenic spacer (IGS) region separating the adjacent units ( Figure 2). The entire ITS fragment (which comprises ITS1, 5.8S rRNA, and ITS2) is typically 500-750 bp long and flanked by the 18S and 28S rRNA genes [61][62][63]. The ITS regions are present in all known fungi and have both highly conserved flanking regions located in the 5.8S, 18S, and 28S rRNA genes as well as the variable regions (located in the ITS1 and ITS2 regions). The conserved flanking regions allowed the development of highly conserved probes or primers to amplify most, if not all, fungi, while the variable regions allowed the development of species-specific markers [64,65]. Together, these features have contributed to ITS being the consensus fungal DNA barcode for the mycological community [64,65]. Furthermore, the ITS sequences obtained from the direct amplification and sequencing of environmental DNA samples have contributed to our increased understanding of fungal diversity from a variety of environments, including those from diseased plants and animals [65,66].
primers to amplify most, if not all, fungi, while the variable regions allowed the development of species-specific markers [64,65]. Together, these features have contributed to ITS being the consensus fungal DNA barcode for the mycological community [64,65]. Furthermore, the ITS sequences obtained from the direct amplification and sequencing of environmental DNA samples have contributed to our increased understanding of fungal diversity from a variety of environments, including those from diseased plants and animals [65,66]. Indeed, our literature analysis showed that over 80% of fungal pathogens infecting bast fiber crops were identified based on PCR-based assays targeting the ITS regions. For example, Wang et al. [29] first identified C. gloeosporioides and C. higginsianum as the agents of anthracnose disease on ramie Indeed, our literature analysis showed that over 80% of fungal pathogens infecting bast fiber crops were identified based on PCR-based assays targeting the ITS regions. For example, Wang et al. [29] first identified C. gloeosporioides and C. higginsianum as the agents of anthracnose disease on ramie plants in China based on ITS sequences [29]. Similarly, Serdani et al. [24,25] obtained ITS sequences and reported that C. phormii was the main agent causing anthracnose on New Zealand flax in the United States and Australia [24,25]. Other fungal pathogens infecting bast fiber crops identified based on ITS sequences include G. cichoracearum, A. alternata, P. aphanidermatum, P. ultimum, and F. oxysporum etc. [35,40,44,46]. Aside from species identification, variations in ITS sequences have also been used to reveal new species and understand the relationships among broad groups of fungi, including those causing diseases in bast fiber crops. For example, based on ITS sequence information, Kwon et al. [28] found a potential new species in the genus Colletotrichum causing kenaf anthracnose disease outbreaks in Korea during the summers of 2013 and 2014 [28]. This new species had an ITS sequence distinct from Colletotrichum acutatum, its closest related species.
However, in certain situations, ITS sequences alone are insufficient for species identification and for revealing the phylogenetic relationships among strains and species of fungi. Thus, it is common nowadays to combine ITS sequence information with those from other gene fragments. Several genes have been commonly used, including those coding for translation elongation factor 1-α (TEF1-α or EF-1α) [32], ß-tubulin (TUB) [42], calmodulin (CAL) [50], histone subunit 3 (H3) [48], glyceraldehydes-3-phosphate dehydrogenase (GAPDH) [47], and actin (ACT) [48]. By sequencing the ITS and 28S regions with primers PM5G/NLP2, Szarka et al. [43] made the first documented report of G. spadiceus causing powdery mildew on industrial hemp in the United States [43]. Yu et al. identified A. alternata as the agent of black leaf spot disease on ramie plants in China on the basis of morphology and DNA sequences at the ITS and GAPDH genes [47]. Similarly, based on the phylogenetic analysis of ITS and EF-1α, Zamir et al. [32] revealed that several Fusarium and Pythium species affecting cannabis plants shared 99%-100% sequence identities with isolates causing stem rot and wilt in other plants, consistent with the broad host range of many of these plant fungal pathogens.
Within the ribosomal RNA gene cluster, aside from the ITS regions, several other regions including 18S, 5.8S, 28S, and IGS regions, have also been used as PCR targets for the detection of fungal pathogens in bast fiber crops [61]. Each of these gene fragments contain relatively conserved regions that are valuable for species-specific primer designs and variable regions for studying related taxonomic groups [65]. In 1997, based on sequencing of a region of the 28S rRNA gene with primers LROR and LR7, McPartland et al. [52] proposed two new species Micropeltopsis cannabis sp. nov. and Orbilia luteola comb. nov., revised the list of five new fungal-Cannabis associations, and reported three known fungal pathogens impacting Cannabis plants for the first time at several new locations [52].
After ITS, the EF-1α gene is the second most common target gene for molecular diagnosis of bast fiber fungal pathogens ( Table 2). The EF-1α gene is a single-copy nuclear protein-coding gene with highly conserved sequences among species. It is a secondary DNA barcode for many groups of fungi [65], often used in phylogenetic studies of divergent fungal groups. Although the database of EF-1α sequences is not as large as that for ITS sequences, EF-1α often contains more variable nucleotide sites than that of ITS and thus can be particularly useful for separating closely related organisms [65,66]. For example, the ITS sequences are often not informative for distinguishing closely related species in Fusarium while on the other hand, EF-1α sequences can [66]. Zamir et al. [32,34] compared F. oxysporum and F. brachygibbosum isolates from cannabis plants in northern California (USA) with all other Fusarium formae speciales and isolates previously recovered from British Columbia (Canada) using sequences at EF-1α and ITS regions [32,34]. They found that a diversity of fungal pathogens can cause hemp root and crown rot under field conditions. However, phylogenetic analysis of EF-1α and ITS sequences revealed that Fusarium species, such as F. oxysporum and F. solani, were the primary fungal pathogens impacting hydroponically grown cannabis plants and that these strains of F. oxysporum and F. solani shared 99%-100% sequence identity with those causing stem rot and wilt in other host plants. Similarly, based on EF-1α and CAL sequences, Casano et al. [50] identified that Macrophomina phaseolina was the agent of charcoal rot on hemp cultivated in southern Spain [ Table 2].
As an important component of the cytoskeleton and spindle microtubules, the highly conserved ß-tubulin gene has been another marker gene for identifying plant fungal pathogens [67]. For example, in 2011, Debode et al. [39] developed a qPCR method to detect several species in Verticillium based on the ß-tubulin gene [39]. Their method allowed the differentiation of the species V. tricorpus, V. dahliae, and V. longisporum in one day. On the other hand, Wang et al. relied on concatenated sequences of ß-tubulin and EF-1α sequences to identify a new sub-species of F. udum f. sp. Crotalariae causing sunn hemp Fusarium wilt in Taiwan [42].
As mentioned above and reviewed elsewhere (e.g., [65]), several other genes have also been used as target DNA in molecular identification for fungal pathogens [68,69]. Those frequently utilized DNA fragments are in conserved genes such as CAL, H3, GDP, and ACT. However, these genes generally appeared in the form of multi-locus sequence typing (MLST) [70,71], and few are used individually in the diagnosis of fungal pathogens in bast fiber crops.
MLST is also known as multi-locus sequence analysis (MLSA) or multiple gene genealogical analysis (MGGA) [70,71]. As the name suggests, MLSA usually analyzes a combination of multiple (usually five to seven) genes (typically housekeeping genes) for each strain. As a result, polymorphisms from all gene fragments can be used together to allow for greater discrimination of strains and species than single gene sequences [71][72][73]. This approach has been used by Doyle et al. [48] in their analysis of fungal pathogens causing leaf spot diseases in industrial hemp fields in Kentucky in 2015. Specifically, they obtained sequences for parts of CAL, H3, ACT, and EF1-α genes and the complete ITS sequences for their strains and identified Cercospora cf. flagellaris as the causal agent of leaf spot disease in their fields [48]. Similarly, based on sequences at six loci (ACT, TUB2, CAL, GS, GAPDH, and ITS), Niu et al. revealed that C. fructicola, C. siamense, and C. corchorumcapsularis sp. nov. were associated with jute anthracnose in southeastern China [26,27].

Conclusions and Future Prospects
As shown above, fungal pathogens of bast fiber crops are very diverse in their taxonomic distributions, ecological niches, and host ranges. Most of these fungi can exist both as saprophytes and as pathogens. Morphologically, they can exist in different forms such as the sexual or asexual spore form and/or the hyphal form (for filamentous and dimorphic fungi). Physiologically, these fungi may be highly active, growing and dividing exponentially, or inactive, in dormant/resting state in natural environments. In addition, they may exist at very high or very low concentrations, or anywhere in between. Some of these fungi are easily culturable in the lab while others may be difficult to cultivate or even unculturable. Most microbial communities in nature, including those on diseased bast fiber crops, often contain a mixture of bacterial and fungal species. As a result, it can be extremely challenging to isolate certain fungal pathogens and identify them based on their cultural and other characteristics. Thus, having a molecular-based method can significantly enhance the detection and diagnosis of pathogens directly from the environment, including diseased plants.
At present, even though progress has been made in the development of molecular diagnosis tools, most diagnoses of bast fiber diseases still rely on disease symptoms and, when available, cultural characteristics on artificial media. However, these features are often not species-specific and often take a long time to obtain. One big advantage of molecular methods over traditional methods is that the molecular methods can be directly applied to plant and soil samples to obtain early detection of potentially devastating and persistent fungal pathogens, even when physiological symptoms are not visible or in a latent phase on crops. Furthermore, molecular markers can contribute to revealing variations among strains of fungal pathogen populations, including virulence and toxin-producing genes. Indeed, the potential advantages of molecular-based technologies for fungal pathogen detection and identification are enormous.
Over the past few years, effective amplification platforms, probe development, and various quantitative PCR technologies have revolutionized research on fungal pathogen detection. From basic research to point-of-care diagnosis, the latest assays and technologies have laid a solid foundation for developing effective fungal detection systems, including those for bast fiber crop-specific fungal diseases. At present, the detection and diagnosis of fungal pathogens in bast fiber crops lag far behind those for several other groups of fungi. Although many types of molecular assays have been developed in recent years, most still rely on pure fungal cultures and require sophisticated equipment. At present, the dominant molecular methods for diagnosing fungal pathogens on bast fiber crops are conventional PCR followed by DNA sequencing. Indeed, there were only a few instances where the more sensitive and species-specific real-time PCR methods were developed for direct identification of fungal pathogens. Future research should aim to develop cost-effective methods that can work directly on diseased plant tissues in field conditions. Indeed, there is great potential for the development of technologies targeting fungal pathogens of bast fiber crops in field conditions. The potential technologies include IC-PCR, PCR-ELISA, dd-PCR, multiplex PCR, LAMP, and DNA microarray [14]. These approaches can increase both the sensitivity and specificity of molecular detection systems. With increasing genomic information from these fungal pathogens, unique markers for each species (or even sub-species) should be easily identifiable and be developed for effective identification of fungal pathogen species and genotypes infecting bast fiber crops.