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Open AccessArticle

Putative Role of a Yet Uncharacterized Protein Elicitor PeBb1 Derived from Beauveria bassiana ARSEF 2860 Strain against Myzus persicae (Homoptera: Aphididae) in Brassica rapa ssp. pekinensis

1
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China
2
Department of Entomology, College of Agriculture, University of Sargodha, Sargodha 40100, Pakistan
3
Department of Entomology, University of Agriculture, Faisalabad 38000, Pakistan
4
Research Institute of Pomology, Chinese Academy of Agricultural Science, Ministry of Agriculture, Xingcheng 125100, China
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(2), 111; https://doi.org/10.3390/pathogens9020111
Received: 11 December 2019 / Revised: 23 January 2020 / Accepted: 5 February 2020 / Published: 11 February 2020
(This article belongs to the Special Issue Plant Resistance Induced by Microorganisms and Pathogens)
This study reports the characterization of protein elicitor PeBb1 derived from entomopathogenic fungus Beauveria bassiana ARSEF-2860 strain and its putative role in induced systemic resistance in Brassica rapa ssp. pekinensis against green peach aphid Myzus persicae. The sequence of purified elicitor protein was matched with the genomic sequence of a hypothetical protein BBA_10269 from B. bassiana ARSEF-2860 (GenBank Accession No. XP_008603588.1). The protein-encoding gene PeBb1 contained 534 bp cDNA encoding a polypeptide of 177 amino acids with a molecular mass of 19 kDa. The recombinant elicitor protein was expressed in Escherichia coli using pET-28a (+) expression vector and induced necrosis in the leaves of tobacco. The effects of elicitor protein on aphid M. persicae was determined by applying three different concentrations of PeBb1 (i.e., 26, 35, 53 μM) on B. rapa plants at 4-leaf stage and the treated plants were exposed to newly emerged (0–6 h old) apterous adult aphids. Bioassay results showed significant (p < 0.05) sub-lethal effects of the exogenous application of PeBb1 elicitor on M. persicae. Moreover, the RT-qPCR gene expression analyses showed a significant up-regulation of most of the key genes linked to ethylene (ET)- and jasmonic acid (JA)-associated plant defense pathways in elicitor-treated plants. These results not only recommend the putative utilization of PeBb1 elicitor protein in future biological pest control strategies against phloem-feeding insect pests such as M. persicae, but also help in better comprehension of the mechanisms through which beneficial fungi trigger the induced plant resistance.
Keywords: Beauveria bassiana; elicitor protein; induced systemic resistance; Myzus persicae; fecundity; jasmonic acid pathway; ethylene pathway Beauveria bassiana; elicitor protein; induced systemic resistance; Myzus persicae; fecundity; jasmonic acid pathway; ethylene pathway
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Nazir, T.; Hanan, A.; Basit, A.; Majeed, M.Z.; Anwar, T.; Nawaz, I.; Qiu, D. Putative Role of a Yet Uncharacterized Protein Elicitor PeBb1 Derived from Beauveria bassiana ARSEF 2860 Strain against Myzus persicae (Homoptera: Aphididae) in Brassica rapa ssp. pekinensis. Pathogens 2020, 9, 111.

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