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Open AccessArticle

Differences in Epstein-Barr Virus Characteristics and Viral-Related Microenvironment Could Be Responsible for Lymphomagenesis in Children

1
Multidisciplinary Institute for Investigation in Pediatric Pathologies (IMIPP), CONICET-GCBA. Molecular Biology Laboratory, Pathology Division, Ricardo Gutiérrez Children’s Hospital, Buenos Aires C1425EFD, Argentina
2
Multidisciplinary Institute for Investigation in Pediatric Pathologies (IMIPP), CONICET-GCBA. Pathology Division, Ricardo Gutiérrez Children’s Hospital, Buenos Aires C1425EFD, Argentina
*
Author to whom correspondence should be addressed.
Authors contributed equally.
Pathogens 2020, 9(1), 68; https://doi.org/10.3390/pathogens9010068
Received: 20 November 2019 / Revised: 13 December 2019 / Accepted: 17 December 2019 / Published: 19 January 2020
(This article belongs to the Section Human Pathogens)
In Argentina, Epstein-Barr virus (EBV) presence is associated with Hodgkin lymphoma (HL) in patients younger than 10 years, suggesting a relationship between low age of EBV infection and HL. Given that HL is derived from germinal centers (GC), our aim was to compare EBV protein expression and microenvironment markers between pediatric HL patients and EBV+GC in children. Methods: EBV presence and immune cell markers were assessed by in situ hybridization and immunohistochemistry (IHC). Results: Viral latency II pattern was proved in all HL patients and in 81.8% of EBV+ tonsillar GCs. LMP1 and LMP2 co-expression were proved in 45.7% HL cases, but only in 7.7% EBV+ GC in pediatric tonsils. An increase in CD4+, IL10, and CD68+ cells was observed in EBV+ GC. In pediatric HL patients, only the mean of IL10+ cells was statistically higher in EBV+ HL. Conclusions: Our findings point us out to suggest that LMP1 expression may be sufficient to drive neoplastic transformation, that an immune regulatory milieu counteracts cytotoxic environment in EBV-associated Hodgkin lymphoma, and that CD4+ and CD68+ cells may be recruited to act in a local collaborative way to restrict, at least in part, viral-mediated lymphomagenesis in tonsillar GC. View Full-Text
Keywords: EBV; Hodgkin lymphoma; germinal center; microenvironment; children EBV; Hodgkin lymphoma; germinal center; microenvironment; children
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MDPI and ACS Style

Vistarop, A.; Jimenez, O.; Cohen, M.; De Matteo, E.; Preciado, M.V.; Chabay, P. Differences in Epstein-Barr Virus Characteristics and Viral-Related Microenvironment Could Be Responsible for Lymphomagenesis in Children. Pathogens 2020, 9, 68.

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