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Open AccessArticle

Staphylococcus saprophyticus Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical Strains Related to Virulence and Persistence

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Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia-GO 74690-900, Brazil
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Departamento de Biologia, Universidade Federal de Lavras, Lavras 37200-900, Brazil
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Laboratório de Química de Proteínas, Instituto de Biologia, Universidade de Brasília, UnB-Brasilia 70910-900, Brazil
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Laboratório de Microbiologia Molecular, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro-RJ 21941-902, Brazil
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(1), 69; https://doi.org/10.3390/pathogens9010069
Received: 17 December 2019 / Revised: 6 January 2020 / Accepted: 10 January 2020 / Published: 19 January 2020
(This article belongs to the Section Human Pathogens)
Staphylococcus saprophyticus is a Gram-positive and coagulase negative cocci that composes the skin microbiota and can act as an opportunistic agent causing urinary tract infections, being more frequent in sexually active young women. The ability of a pathogen to cause infection in the host is associated to its ability to adhere to host cells and to survive host immune defenses. In this work, we presented the comparative proteomic profile of three S. saprophyticus strains. It was possible to characterize differences in the proteome content, specially related to expression of virulence factors. We compiled this data and previous data and we detected one strain (9325) possessing higher production and secretion of proteins related to virulence. Our results show that phenotypic, genotypic, and proteomic differences reflect in the ability to survive during interaction with host cells, since the 9325 strain presented a higher survival rate after macrophage interaction. In counterpart, the 7108 strain that possesses lower content of proteins related to virulence presented higher ability to form biofilm suggesting that this strain can be better adapted to persist in the host and in the environment. Our work describes, for the first time, proteomic flexibility among S. saprophyticus strains, reflecting in virulence and persistence. View Full-Text
Keywords: proteome; proteomic flexibility; virulence; urease; thioredoxin; biofilm proteome; proteomic flexibility; virulence; urease; thioredoxin; biofilm
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Silva, K.C.S.; Silva, L.O.S.; Silva, G.A.A.; Borges, C.L.; Novaes, E.; Paccez, J.D.; Fontes, W.; Giambiagi-deMarval, M.; Soares, C.M.A.; Parente-Rocha, J.A. Staphylococcus saprophyticus Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical Strains Related to Virulence and Persistence. Pathogens 2020, 9, 69.

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