Next Article in Journal
Effects of Shape and Size of Cobalt Phosphate Nanoparticles against Acanthamoeba castellanii
Previous Article in Journal
Antiviral Activity of Germacrone against Pseudorabies Virus in Vitro
Open AccessArticle

Entry of Scotophilus Bat Coronavirus-512 and Severe Acute Respiratory Syndrome Coronavirus in Human and Multiple Animal Cells

Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 32020, Taiwan
*
Author to whom correspondence should be addressed.
Pathogens 2019, 8(4), 259; https://doi.org/10.3390/pathogens8040259
Received: 25 October 2019 / Revised: 20 November 2019 / Accepted: 20 November 2019 / Published: 22 November 2019
(This article belongs to the Section Animal Pathogens)
Bats are natural reservoirs of severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome CoV (MERS-CoV). Scotophilus bat CoV-512 demonstrates potential for cross-species transmission because its viral RNA and specific antibodies have been detected in three bat species of Taiwan. Understanding the cell tropism of Scotophilus bat CoV-512 is the first step for studying the mechanism of cross-species transmission. In this study, a lentivirus-based pseudovirus was produced using the spike (S) protein of Scotophilus bat CoV-512 or SARS-CoV as a surface protein to test the interaction between coronaviral S protein and its cell receptor on 11 different cells. Susceptible cells expressed red fluorescence protein (RFP) after the entry of RFP-bound green fluorescence protein (GFP)-fused S protein of Scotophilus bat CoV-512 (RFP-Sco-S-eGFP) or RFP-SARS-S pseudovirus, and firefly luciferase (FLuc) activity expressed by cells infected with FLuc-Sco-S-eGFP or FLuc-SARS-S pseudovirus was quantified. Scotophilus bat CoV-512 pseudovirus had significantly higher entry efficiencies in Madin Darby dog kidney epithelial cells (MDCK), black flying fox brain cells (Pabr), and rat small intestine epithelial cells (IEC-6). SARS-CoV pseudovirus had significantly higher entry efficiencies in human embryonic kidney epithelial cells (HEK-293T), pig kidney epithelial cells (PK15), and MDCK cells. These findings demonstrated that Scotophilus bat CoV-512 had a broad host range for cross-species transmission like SARS-CoV. View Full-Text
Keywords: Scotophilus bat coronavirus-512; severe acute respiratory syndrome-coronavirus; pseudovirus; cell receptor Scotophilus bat coronavirus-512; severe acute respiratory syndrome-coronavirus; pseudovirus; cell receptor
Show Figures

Figure 1

MDPI and ACS Style

Chen, Y.-N.; Hsu, H.-C.; Wang, S.-W.; Lien, H.-C.; Lu, H.-T.; Peng, S.-K. Entry of Scotophilus Bat Coronavirus-512 and Severe Acute Respiratory Syndrome Coronavirus in Human and Multiple Animal Cells. Pathogens 2019, 8, 259.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop