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Open AccessArticle

Host-Pathogen Interactions of Mycoplasma mycoides in Caprine and Bovine Precision-Cut Lung Slices (PCLS) Models

1
Institute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, 30173 Hannover, Germany
2
Institute of Immunology, Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, 17493 Greifswald-Insel Riems, Germany
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Institute for Pathology, University of Veterinary Medicine Hannover, 30559 Hannover, Germany
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Institute of Anatomy, University of Veterinary Medicine Hannover, 30173 Hannover, Germany
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Institute of Veterinary Bacteriology, University of Bern, CH-3001 Bern, Switzerland
6
International Livestock Research Institute, PO Box 30709, 00100 Nairobi, Kenya
*
Author to whom correspondence should be addressed.
Pathogens 2019, 8(2), 82; https://doi.org/10.3390/pathogens8020082
Received: 29 May 2019 / Revised: 13 June 2019 / Accepted: 18 June 2019 / Published: 20 June 2019
(This article belongs to the Section Animal Pathogens)
Respiratory infections caused by mycoplasma species in ruminants lead to considerable economic losses. Two important ruminant pathogens are Mycoplasma mycoides subsp. Mycoides (Mmm), the aetiological agent of contagious bovine pleuropneumonia and Mycoplasma mycoides subsp. capri (Mmc), which causes pneumonia, mastitis, arthritis, keratitis, and septicemia in goats. We established precision cut lung slices (PCLS) infection model for Mmm and Mmc to study host-pathogen interactions. We monitored infection over time using immunohistological analysis and electron microscopy. Moreover, infection burden was monitored by plating and quantitative real-time PCR. Results were compared with lungs from experimentally infected goats and cattle. Lungs from healthy goats and cattle were also included as controls. PCLS remained viable for up to two weeks. Both subspecies adhered to ciliated cells. However, the titer of Mmm in caprine PCLS decreased over time, indicating species specificity of Mmm. Mmc showed higher tropism to sub-bronchiolar tissue in caprine PCLS, which increased in a time-dependent manner. Moreover, Mmc was abundantly observed on pulmonary endothelial cells, indicating partially, how it causes systemic disease. Tissue destruction upon prolonged infection of slices was comparable to the in vivo samples. Therefore, PCLS represents a novel ex vivo model to study host-pathogen interaction in livestock mycoplasma. View Full-Text
Keywords: Mycoplasma mycoides; CBPP; MAKePS syndrome; PCLS; tropism; immunofluorescence; 3R Mycoplasma mycoides; CBPP; MAKePS syndrome; PCLS; tropism; immunofluorescence; 3R
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Weldearegay, Y.B.; Müller, S.; Hänske, J.; Schulze, A.; Kostka, A.; Rüger, N.; Hewicker-Trautwein, M.; Brehm, R.; Valentin-Weigand, P.; Kammerer, R.; Jores, J.; Meens, J. Host-Pathogen Interactions of Mycoplasma mycoides in Caprine and Bovine Precision-Cut Lung Slices (PCLS) Models. Pathogens 2019, 8, 82.

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