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Volume 13
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10.3390/pathogens13080700
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Article

Phylogenetics and Mobilization of Genomic Traits of Cephalosporin-Resistant Escherichia coli Originated from Retail Meat

by
Ewelina Iwan
1,*,
Magdalena Zając
2,
Arkadiusz Bomba
1,
Małgorzata Olejnik
1,3,
Magdalena Skarżyńska
2,
Bernard Wasiński
2,
Kinga Wieczorek
4,
Katarzyna Tłuścik
1 and
Dariusz Wasyl
1,2
1
Department of Omics Analyses, National Veterinary Research Institute, 57 Partyzantow, 24-100 Pulawy, Poland
2
Department of Microbiology, National Veterinary Research Institute, 57 Partyzantow, 24-100 Pulawy, Poland
3
Faculty of Biological and Veterinary Sciences, Department of Basic and Preclinical Sciences, Nicolaus Copernicus University in Torun, 11 Gagarina St., 87-100 Torun, Poland
4
Department of Food of Safety, National Veterinary Research Institute, 57 Partyzantow, 24-100 Pulawy, Poland
*
Author to whom correspondence should be addressed.
Pathogens 2024, 13(8), 700; https://doi.org/10.3390/pathogens13080700
Submission received: 8 July 2024 / Revised: 7 August 2024 / Accepted: 13 August 2024 / Published: 19 August 2024
(This article belongs to the Special Issue Virulence and Resistance Mechanisms in Multidrug-Resistant Gram-Negative Bacteria)
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Abstract

Contaminations with cephalosporin-resistant Escherichia coli across the food chain may pose a significant threat to public health because those antimicrobials are critically important in human medicine. The impact of the presented data is especially significant concerning Poland’s role as one of the leading food producers in the EU. This work aimed to characterize the genomic contents of cephalosporin-resistant Escherichia coli (n = 36) isolated from retail meat to expand the official AMR monitoring reported by EFSA. The ESBL mechanism was predominant (via blaCTX-M-1 and blaSHV-12), with the AmpC-type represented by the blaCMY-2 variant. The strains harbored multiple resistance genes, mainly conferring resistance to aminoglycosides, sulfonamides, trimethoprim, tetracyclines. In some isolates, virulence factors—including intimin (eae) and its receptor (tir) were detected, indicating significant pathogenic potential. Resistance genes showed a link with IncI1 and IncB/O/K/Z plasmids. Cephalosporinases were particularly linked to ISEc9/ISEc1 (blaCTX-M-1 and blaCMY-2). The association of virulence with mobile elements was less common—mostly with IncF plasmids. The analysis of E. coli isolated from retail meat indicates accumulation of ARGs and their association with various mobile genetic elements, thus increasing the potential for the transmission of resistance across the food chain.
Keywords: food safety; Escherichia coli; genomics; cephalosporin-resistance; ARGs transmission food safety; Escherichia coli; genomics; cephalosporin-resistance; ARGs transmission

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MDPI and ACS Style

Iwan, E.; Zając, M.; Bomba, A.; Olejnik, M.; Skarżyńska, M.; Wasiński, B.; Wieczorek, K.; Tłuścik, K.; Wasyl, D. Phylogenetics and Mobilization of Genomic Traits of Cephalosporin-Resistant Escherichia coli Originated from Retail Meat. Pathogens 2024, 13, 700. https://doi.org/10.3390/pathogens13080700

AMA Style

Iwan E, Zając M, Bomba A, Olejnik M, Skarżyńska M, Wasiński B, Wieczorek K, Tłuścik K, Wasyl D. Phylogenetics and Mobilization of Genomic Traits of Cephalosporin-Resistant Escherichia coli Originated from Retail Meat. Pathogens. 2024; 13(8):700. https://doi.org/10.3390/pathogens13080700

Chicago/Turabian Style

Iwan, Ewelina, Magdalena Zając, Arkadiusz Bomba, Małgorzata Olejnik, Magdalena Skarżyńska, Bernard Wasiński, Kinga Wieczorek, Katarzyna Tłuścik, and Dariusz Wasyl. 2024. "Phylogenetics and Mobilization of Genomic Traits of Cephalosporin-Resistant Escherichia coli Originated from Retail Meat" Pathogens 13, no. 8: 700. https://doi.org/10.3390/pathogens13080700

APA Style

Iwan, E., Zając, M., Bomba, A., Olejnik, M., Skarżyńska, M., Wasiński, B., Wieczorek, K., Tłuścik, K., & Wasyl, D. (2024). Phylogenetics and Mobilization of Genomic Traits of Cephalosporin-Resistant Escherichia coli Originated from Retail Meat. Pathogens, 13(8), 700. https://doi.org/10.3390/pathogens13080700

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