Colorimetric Kit for Rapid Porcine Circovirus 2 (PCV-2) Diagnosis

Round 1

Reviewer 1 Report

Manuscript entitled „ Colorimetric Kit for Rapid Porcine Circovirus 2 (PCV-2) Diagnosis” is very interesting, well-written and well-planned experimental work. Small corrections should be made to the text  according to the following comments:

Introduction

line 42 – explain in full name abbreviation PCV

line 42,43 – use abbreviation rtPCR after TagManPCR

line 47 – use only abbreviation rtPCR without the full name explanation

line 62 – use only abbreviation AuNPs

line 64 – explain in full name for the first time abbreviation LSPR

line 65 – use only abbreviation SPR instead of full name

Materials and Methods

line 88 – put space between coma and 99%

line 88 - write in the same order as before first abbreviation (MUA) and later concentration 95%

line 97 – write Sigma-Aldrich (Italy)

line 102 – explain in full name abbreviation BSA

Table1 – explain in full name abbreviations qPCR and PCV-2 which for the firs time are used in the description of the table; some number positions use commas, replace them with dots

line 144 – explain in full name abbreviation FTIR

line 146 – live only abbreviation TEM instead of full name

Figure 1  and Figure 2 - explain all the abbreviations that first appeared in the figures

Results and Discussion

Figure 4 – explain abbreviation UV-Vis for the first time

line 204 – explain in full name UV-Vis

Figure 5 - all abbreviations that were not previously used in the description of tables or figures must be explained with the full name

line 239 - replace comma with a dot

line 290 – use only abbreviation LSPR its full name was explained previously in the text

References

bind all items of literature in accordance with the template applicable in the journal

Journal Articles:

1. Author 1, A.B.; Author 2, C.D. Title of the article. Abbreviated Journal Name Year, Volume, page range.

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Reviewer 2 Report

In the article entitled: "Colorimetric Kit for Rapid Porcine Circovirus 2 (PCV-2) Diagnosis" authors provided an interesting and well-designed study regarding the rapid and costless detection of ubiquitous PCV2-virus.

The study provided by authors is interesting, introduction is sufficient, methods are sound, results are clearly described.

Major comments:

1. Please provide photography of log2-sample in comparison to negative control in the figure 8, from the figure 6 we may know that the colour will be probably the same, but this would be helpful.
2. Please provide the information about limit of detection (LOD). In the Figure 6 we may see there is still color-change in 1:10 000 dilution of log2 sample (even better plot shift). It means that sample containing 100 DNA copies/µl is still detectable when diluted to 1 DNA copy/0,01 µ That is quite low and unusual, much lower than qPCR can provide. Please comment that.

Minor comments:

1. Line 57: LSPR
2. Line 288: S1-S3, there is no S4 and S5 available in supplementary file
3. Please adjust the figures, many of them are in different sizes, lower or higher. Please use the same size. Some of them may be moved to supplementary files (i.e Fig 1-3).

I have no further comments.

Author Response

Please see the attachment

Author Response File: Author Response.pdf