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Article

Community Parameters and Genome-Wide RAD-Seq Loci of Ceratothoa oestroides Imply Its Transfer between Farmed European Sea Bass and Wild Farm-Aggregating Fish

1
Institute of Oceanography and Fisheries, Laboratory of Aquaculture, 21000 Split, Croatia
2
Institute of Parasitology, Biology Centre of Czech Academy of Science, 37005 Ceske Budejovice, Czech Republic
3
University Department of Marine Studies, University of Split, 21000 Split, Croatia
4
Hellenic Centre for Marine Research, Institute of Marine Biology, Biotechnology and Aquaculture (IMBBC), 71003 Heraklion, Greece
5
Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling FK9 4LA, UK
*
Author to whom correspondence should be addressed.
Academic Editor: Roberto Bermudez Pose
Pathogens 2021, 10(2), 100; https://doi.org/10.3390/pathogens10020100
Received: 7 December 2020 / Revised: 14 January 2021 / Accepted: 19 January 2021 / Published: 21 January 2021
Wild fish assemblages that aggregate within commercial marine aquaculture sites for feeding and shelter have been considered as a primary source of pathogenic parasites vectored to farmed fish maintained in net pens at an elevated density. In order to evaluate whether Ceratothoa oestroides (Isopoda, Cymothoidae), a generalist and pestilent isopod that is frequently found in Adriatic and Greek stocks of farmed European sea bass (Dicentrarchus labrax), transfers between wild and farmed fish, a RAD-Seq (restriction-site-associated DNA sequencing)-mediated genetic screening approach was employed. The double-digest RAD-Seq of 310 C. oestroides specimens collected from farmed European sea bass (138) and different wild farm-aggregating fish (172) identified 313 robust SNPs that evidenced a close genetic relatedness between the “wild” and “farmed” genotypes. ddRAD-Seq proved to be an effective method for detecting the discrete genetic structuring of C. oestroides and genotype intermixing between two populations. The parasite prevalence in the farmed sea bass was 1.02%, with a mean intensity of 2.0 and mean abundance of 0.02, while in the wild fish, the prevalence was 8.1%; the mean intensity, 1.81; and the mean abundance, 0.15. Such differences are likely a consequence of human interventions during the farmed fish’s rearing cycle that, nevertheless, did not affect the transfer of C. oestroides. View Full-Text
Keywords: Ceratothoa oestroides; Dicentrarchus labrax; mean abundance; mean intensity; parasite transfer; prevalence; RAD-Seq Ceratothoa oestroides; Dicentrarchus labrax; mean abundance; mean intensity; parasite transfer; prevalence; RAD-Seq
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MDPI and ACS Style

Mladineo, I.; Hrabar, J.; Trumbić, Ž.; Manousaki, T.; Tsakogiannis, A.; Taggart, J.B.; Tsigenopoulos, C.S. Community Parameters and Genome-Wide RAD-Seq Loci of Ceratothoa oestroides Imply Its Transfer between Farmed European Sea Bass and Wild Farm-Aggregating Fish. Pathogens 2021, 10, 100. https://doi.org/10.3390/pathogens10020100

AMA Style

Mladineo I, Hrabar J, Trumbić Ž, Manousaki T, Tsakogiannis A, Taggart JB, Tsigenopoulos CS. Community Parameters and Genome-Wide RAD-Seq Loci of Ceratothoa oestroides Imply Its Transfer between Farmed European Sea Bass and Wild Farm-Aggregating Fish. Pathogens. 2021; 10(2):100. https://doi.org/10.3390/pathogens10020100

Chicago/Turabian Style

Mladineo, Ivona, Jerko Hrabar, Željka Trumbić, Tereza Manousaki, Alexandros Tsakogiannis, John B. Taggart, and Costas S. Tsigenopoulos 2021. "Community Parameters and Genome-Wide RAD-Seq Loci of Ceratothoa oestroides Imply Its Transfer between Farmed European Sea Bass and Wild Farm-Aggregating Fish" Pathogens 10, no. 2: 100. https://doi.org/10.3390/pathogens10020100

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