Comparison of MT-PCR with Quantitative PCR for Human Bocavirus in Respiratory Samples with Multiple Respiratory Viruses Detection

Mijač, Maja; Ljubin-Sternak, Sunčanica; Ivković-Jureković, Irena; Vraneš, Jasmina

doi:10.3390/diagnostics13050846

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Comparison of MT-PCR with Quantitative PCR for Human Bocavirus in Respiratory Samples with Multiple Respiratory Viruses Detection

by

Maja Mijač

^1,2,*,

Sunčanica Ljubin-Sternak

^1,2

,

Irena Ivković-Jureković

^3,4 and

Jasmina Vraneš

^1,2

¹

Molecular Microbiology Department, Dr. Andrija Štampar Teaching Institute of Public Health, 10000 Zagreb, Croatia

²

Medical Microbiology Department, University of Zagreb School of Medicine, 10000 Zagreb, Croatia

³

Department of Pulmonology, Allergy, Immunology and Rheumatology, Children’s Hospital Zagreb, 10000 Zagreb, Croatia

⁴

Faculty for Dental Medicine and Healthcare, School of Medicine, Josip Juraj Strossmayer University of Osijek, 31000 Osijek, Croatia

^*

Author to whom correspondence should be addressed.

Diagnostics 2023, 13(5), 846; https://doi.org/10.3390/diagnostics13050846

Submission received: 26 December 2022 / Revised: 6 February 2023 / Accepted: 21 February 2023 / Published: 23 February 2023

(This article belongs to the Special Issue Pediatric Diagnostic Microbiology)

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Abstract

Human bocavirus (HBoV) is an important respiratory pathogen, especially in children, but it is often found in co-detection with other respiratory viruses, which makes the diagnostic approach challenging. We compared multiplex PCR and quantitative PCR for HBoV with multiplex tandem PCR (MT-PCR) in 55 cases of co-detection of HBoV and other respiratory viruses. In addition, we investigated whether there is a connection between the severity of the disease, measured by the localization of the infection, and amount of virus detected in the respiratory secretions. No statistically significant difference was found, but children with large amount of HBoV and other respiratory virus had a longer stay in hospital.

Keywords: human bocavirus; children; respiratory tract; viral load; MT-PCR

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MDPI and ACS Style

Mijač, M.; Ljubin-Sternak, S.; Ivković-Jureković, I.; Vraneš, J. Comparison of MT-PCR with Quantitative PCR for Human Bocavirus in Respiratory Samples with Multiple Respiratory Viruses Detection. Diagnostics 2023, 13, 846. https://doi.org/10.3390/diagnostics13050846

AMA Style

Mijač M, Ljubin-Sternak S, Ivković-Jureković I, Vraneš J. Comparison of MT-PCR with Quantitative PCR for Human Bocavirus in Respiratory Samples with Multiple Respiratory Viruses Detection. Diagnostics. 2023; 13(5):846. https://doi.org/10.3390/diagnostics13050846

Chicago/Turabian Style

Mijač, Maja, Sunčanica Ljubin-Sternak, Irena Ivković-Jureković, and Jasmina Vraneš. 2023. "Comparison of MT-PCR with Quantitative PCR for Human Bocavirus in Respiratory Samples with Multiple Respiratory Viruses Detection" Diagnostics 13, no. 5: 846. https://doi.org/10.3390/diagnostics13050846

APA Style

Mijač, M., Ljubin-Sternak, S., Ivković-Jureković, I., & Vraneš, J. (2023). Comparison of MT-PCR with Quantitative PCR for Human Bocavirus in Respiratory Samples with Multiple Respiratory Viruses Detection. Diagnostics, 13(5), 846. https://doi.org/10.3390/diagnostics13050846

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Comparison of MT-PCR with Quantitative PCR for Human Bocavirus in Respiratory Samples with Multiple Respiratory Viruses Detection

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