Evaluation of Carbonic Anhydrase, Acetylcholinesterase, Butyrylcholinesterase, and α-Glycosidase Inhibition Effects and Antioxidant Activity of Baicalin Hydrate
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Fe3+ Reducing Ability Assays
2.3. Cu2+ Reducing Ability Assays
2.4. DPPH• Radical Scavenging Activities
2.5. ABTS•+ Radical Scavenging Activities
2.6. DMPD•+ Radical Scavenging Activities
2.7. Acetylcholinesterase/Butyrylcholinesterase Enzymes Inhibition Assay
2.8. α-Glycosidase Enzyme Inhibition Assay
2.9. Human Carbonic Anhydrase II Isoenzyme Inhibition Assay
2.10. IC50 Values Determination
2.11. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Antioxidants | Fe3+ Reducing | Cu2+ Reducing | ||
---|---|---|---|---|
λ (700 nm) | r2 | λ (450 nm) | r2 | |
BHA | 2.347 | 0.9086 | 2.216 | 0.9928 |
BHT | 0.952 | 0.9154 | 2.044 | 0.9937 |
Trolox | 2.119 | 0.9586 | 1.548 | 0.9305 |
α-Tocopherol | 0.957 | 0.9863 | 0.816 | 0.9897 |
Baicalin hydrate | 1.249 | 0.9848 | 0.344 | 0.9517 |
Antioxidants | DPPH• Scavenging | ABTS•+ Scavenging | DMPD•+ Scavenging | |||
---|---|---|---|---|---|---|
IC50 | r2 | IC50 | r2 | IC50 | r2 | |
BHA | 10.10 | 0.9015 | 5.07 | 0.9356 | 11.99 | 0.9580 |
BHT | 25.95 | 0.9221 | 6.99 | 0.9350 | 8.72 | 0.9375 |
Trolox | 7.05 | 0.9614 | 6.16 | 0.9692 | 4.33 | 0.9447 |
α-Tocopherol | 11.31 | 0.9642 | 8.37 | 0.9015 | 7.11 | 0.9509 |
Baicalin hydrate | 13.40 | 0.9940 | 38.37 | 0.9888 | 90.91 | 0.7410 |
Compounds | IC50 (nM) | Ki (nM, n: 3) | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
CA II | r2 | AChE | r2 | BChE | r2 | α-Gly | r2 | CA II | AChE | BChE | α-Gly | |
Baicalin hydrate | 31.13 | 0.9562 | 14.65 | 0.9758 | 12.31 | 0.9524 | 24.62 | 0.9524 | 19.25 ± 1.79 | 10.01 ± 2.86 | 3.50 ± 0.68 | 26.98 ± 9.91 |
Acetazolamide * | 8.37 | 0.9825 | - | - | - | - | - | - | 4.41 ± 0.35 | - | - | - |
Tacrine ** | - | - | 5.97 | 0.9706 | 8.37 | 0.9846 | - | - | - | 2.43 ± 0.92 | 5.99 ± 1.79 | - |
Acarbose *** | - | - | - | - | - | - | 22,800 | - | - | - | - | - |
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Durmaz, L.; Karagecili, H.; Gulcin, İ. Evaluation of Carbonic Anhydrase, Acetylcholinesterase, Butyrylcholinesterase, and α-Glycosidase Inhibition Effects and Antioxidant Activity of Baicalin Hydrate. Life 2023, 13, 2136. https://doi.org/10.3390/life13112136
Durmaz L, Karagecili H, Gulcin İ. Evaluation of Carbonic Anhydrase, Acetylcholinesterase, Butyrylcholinesterase, and α-Glycosidase Inhibition Effects and Antioxidant Activity of Baicalin Hydrate. Life. 2023; 13(11):2136. https://doi.org/10.3390/life13112136
Chicago/Turabian StyleDurmaz, Lokman, Hasan Karagecili, and İlhami Gulcin. 2023. "Evaluation of Carbonic Anhydrase, Acetylcholinesterase, Butyrylcholinesterase, and α-Glycosidase Inhibition Effects and Antioxidant Activity of Baicalin Hydrate" Life 13, no. 11: 2136. https://doi.org/10.3390/life13112136