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Article

Comparison of Chicken Immune Responses to Immunization with Vaccine La Sota or ZG1999HDS Strain of Newcastle Disease Virus

1
Veterinary and Food Safety Directorate General, Ministry of Agriculture, 10 000 Zagreb, Croatia
2
Faculty of Veterinary Medicine, University of Zagreb, 10 000 Zagreb, Croatia
3
Laboratory of Immunology, Centre for Research and Knowledge Transfer in Biotechnology, University of Zagreb, 10 000 Zagreb, Croatia
4
Department of Immunobiology, The University of Arizona College of Medicine, Tucson, AZ 85719, USA
5
Department of Poultry Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, 10 000 Zagreb, Croatia
*
Authors to whom correspondence should be addressed.
Academic Editor: Eric R. May
Life 2022, 12(1), 72; https://doi.org/10.3390/life12010072
Received: 23 October 2021 / Revised: 13 December 2021 / Accepted: 25 December 2021 / Published: 5 January 2022
(This article belongs to the Special Issue Challenges and Control of Newcastle Disease Viruses)
Newcastle disease (ND) is a highly contagious avian disease. Global control of ND is mainly based on vaccination of poultry; however, reported outbreaks of ND in vaccinated flocks indicate a constant need to re-evaluate the existing vaccines and a development of the new ones. In this study, 4-week-old male chickens of the layer commercial hybrid were immunized oculonasally with a commercial NDV live La Sota vaccine (LS group), a suspension of lyophilized NDV strain ZG1999HDS (ZG group), or saline (Control (K) group). Antibody response was determined by haemagglutination inhibition (HI) assay. Cell-mediated immunity (CMI) was characterized by immunophenotyping of leukocyte’s and T-lymphocyte’s subpopulations (flow cytometry). Applied NDV strains did not cause any adverse reaction in treated chickens. Both strains induced the significantly higher HI antibody response in comparison to the control group, and overall antibody titer was higher in ZG group than in LS group. CMI, manifested as a higher proliferation of B- and T-helper cells, yielded better results in the ZG groups than in the LS group. Based on the obtained results, we conclude that the strain ZG1999HDS is immunogenic and is a suitable candidate for further research and development of poultry vaccines. View Full-Text
Keywords: Newcastle disease; ZG1999HDS strain; chicken; immunization; antibody response; cell-mediated immunity Newcastle disease; ZG1999HDS strain; chicken; immunization; antibody response; cell-mediated immunity
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MDPI and ACS Style

Nedeljković, G.; Mazija, H.; Cvetić, Ž.; Jergović, M.; Bendelja, K.; Gottstein, Ž. Comparison of Chicken Immune Responses to Immunization with Vaccine La Sota or ZG1999HDS Strain of Newcastle Disease Virus. Life 2022, 12, 72. https://doi.org/10.3390/life12010072

AMA Style

Nedeljković G, Mazija H, Cvetić Ž, Jergović M, Bendelja K, Gottstein Ž. Comparison of Chicken Immune Responses to Immunization with Vaccine La Sota or ZG1999HDS Strain of Newcastle Disease Virus. Life. 2022; 12(1):72. https://doi.org/10.3390/life12010072

Chicago/Turabian Style

Nedeljković, Gordana, Hrvoje Mazija, Željko Cvetić, Mladen Jergović, Krešo Bendelja, and Željko Gottstein. 2022. "Comparison of Chicken Immune Responses to Immunization with Vaccine La Sota or ZG1999HDS Strain of Newcastle Disease Virus" Life 12, no. 1: 72. https://doi.org/10.3390/life12010072

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