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A (3D-Nuclear) Space Odyssey: Making Sense of Hi-C Maps

Epigenetics and Sex Development Group, Berlin Institute for Medical Systems Biology, Max-Delbrück Center for Molecular Medicine, 10115 Berlin, Germany
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Genes 2019, 10(6), 415; https://doi.org/10.3390/genes10060415
Received: 15 May 2019 / Revised: 26 May 2019 / Accepted: 28 May 2019 / Published: 29 May 2019
(This article belongs to the Special Issue 3D Genomics)
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Abstract

Three-dimensional (3D)-chromatin organization is critical for proper enhancer-promoter communication and, therefore, for a precise execution of the transcriptional programs governing cellular processes. The emergence of Chromosome Conformation Capture (3C) methods, in particular Hi-C, has allowed the investigation of chromatin interactions on a genome-wide scale, revealing the existence of overlapping molecular mechanisms that we are just starting to decipher. Therefore, disentangling Hi-C signal into these individual components is essential to provide meaningful biological data interpretation. Here, we discuss emerging views on the molecular forces shaping the genome in 3D, with a focus on their respective contributions and interdependence. We discuss Hi-C data at both population and single-cell levels, thus providing criteria to interpret genomic function in the 3D-nuclear space. View Full-Text
Keywords: 3D-chromatin organization; long-range gene regulation; chromosome conformation capture 3D-chromatin organization; long-range gene regulation; chromosome conformation capture
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Mota-Gómez, I.; Lupiáñez, D.G. A (3D-Nuclear) Space Odyssey: Making Sense of Hi-C Maps. Genes 2019, 10, 415.

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