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A (3D-Nuclear) Space Odyssey: Making Sense of Hi-C Maps

Epigenetics and Sex Development Group, Berlin Institute for Medical Systems Biology, Max-Delbrück Center for Molecular Medicine, 10115 Berlin, Germany
Author to whom correspondence should be addressed.
Genes 2019, 10(6), 415;
Received: 15 May 2019 / Revised: 26 May 2019 / Accepted: 28 May 2019 / Published: 29 May 2019
(This article belongs to the Special Issue 3D Genomics)
Three-dimensional (3D)-chromatin organization is critical for proper enhancer-promoter communication and, therefore, for a precise execution of the transcriptional programs governing cellular processes. The emergence of Chromosome Conformation Capture (3C) methods, in particular Hi-C, has allowed the investigation of chromatin interactions on a genome-wide scale, revealing the existence of overlapping molecular mechanisms that we are just starting to decipher. Therefore, disentangling Hi-C signal into these individual components is essential to provide meaningful biological data interpretation. Here, we discuss emerging views on the molecular forces shaping the genome in 3D, with a focus on their respective contributions and interdependence. We discuss Hi-C data at both population and single-cell levels, thus providing criteria to interpret genomic function in the 3D-nuclear space. View Full-Text
Keywords: 3D-chromatin organization; long-range gene regulation; chromosome conformation capture 3D-chromatin organization; long-range gene regulation; chromosome conformation capture
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MDPI and ACS Style

Mota-Gómez, I.; Lupiáñez, D.G. A (3D-Nuclear) Space Odyssey: Making Sense of Hi-C Maps. Genes 2019, 10, 415.

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